Mknk2 (NM_001011985) Rat Untagged Clone

CAT#: RN204301

Mknk2 (untagged ORF) - Rat MAP kinase-interacting serine/threonine kinase 2 (Mknk2), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Mknk2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN204301 representing NM_001011985
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCAGAAGAGAACAGCCGAACTTCAGGGCTTCCACCGTTCGTTCAAGGGGCAGAATCCTTTCGAGT
  TGGCCTTCACCTTAGACCCCGCCCAGCACGGGGACTCTGACTTCAGCCCGCAGTGTGAAGCCCGACCTGA
  CATGCCTTCCAGTCAGCCCATCGACATCCCAGACGCCAAGAAGAGAGGCCGCAAAAAGAAGCGCTGTCGG
  GCTACTGACAGCTTCTCAGGCAGATTTGAAGATGTCTACCAGCTGCAGGAGGATGTGCTGGGGGAAGGGG
  CCCATGCCCGTGTGCAGACCTGTGTCAATCTCATCACCAACCAGGAATATGCTGTCAAGATCATTGAGAA
  GCAGCTGGGCCATATCCGCAGCAGGGTGTTCCGGGAGGTGGAGATGCTGTACCAGTGCCAGGGACATAGG
  AATGTTCTAGAACTGATTGAGTTCTTTGAGGAGGAGGACCGCTTCTACCTGGTGTTTGAAAAGATGCGTG
  GTGGATCCATCCTGAGCCACATCCACAGAAGGCGCCACTTTAATGAACTGGAGGCCAGCGTGGTGGTACA
  GGATGTGGCCAGTGCCCTGGACTTCCTGCATAACAAAGGCATCGCCCACAGGGACCTAAAGCCAGAGAAC
  ATCCTGTGTGAGCACCCCAACCAGGTCTCGCCAGTGAAGATCTGTGACTTCGATCTTGGCAGTGGCATTA
  AACTCAACGGAGACTGCTCCCCCATCTCCACGCCAGAGCTGCTCACCCCGTGTGGGTCTGCGGAGTACAT
  GGCACCCGAGGTGGTGGAAGCCTTTAGTGAGGAGGCCAGCATCTATGACAAGCGCTGCGACCTGTGGAGC
  CTGGGTGTCATCCTGTACATCCTGCTCAGTGGCTACCCGCCCTTCGTAGGCCACTGTGGCAGCGACTGTG
  GCTGGGACCGCGGGGAGGCCTGCCCTGCCTGCCAGAACATGCTGTTTGAGAGCATCCAGGAAGGGAAGTA
  CGAGTTCCCCGACAAGGACTGGTCCCACATCTCCTTCGCTGCCAAAGACCTCATCTCCAAGCTGCTGGTG
  CGAGATGCCAAGCAGAGGCTGAGCGCTGCCCAAGTTCTGCAGCACCCCTGGGTGCAGGGGTGTGCTCCAG
  AGAACACCTTACCAACACCCTTGGTTCTGCAGAGGAACAGCTGTGCCAAAGACCTCACGTCCTTTGCAGC
  TGAGGCCATCGCCATGAACCGGCAGCTGGCCCAGTGTGAGGAGGACGCTGGGCAGGACCAGCCTGTGCTC
  ATCCGAGCTACCTCACGCTGCCTGCAGTTGTCCCCGCCCTCCCAGTCCAAGCTGGCCCAGAGGCGCCAGA
  GGGCCAGCCTGTCGGCCACCCCTGTGGTCCTCGTAGGGGATCGCGTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001011985
• Insert Size: 1380 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001011985.1, NP_001011985.1
• RefSeq Size: 3458 bp
• RefSeq ORF: 1380 bp
• Locus ID: 299618
• UniProt ID: Q5U2N4
• Cytogenetics: 7q11
• Gene Summary: Serine/threonine-protein kinase that phosphorylates SFPQ/PSF, HNRNPA1 and EIF4E. May play a role in the response to environmental stress and cytokines. Appears to regulate translation by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap. Required for mediating PP2A-inhibition-induced EIF4E phosphorylation. Triggers EIF4E shuttling from cytoplasm to nucleus. Enhances the formation of EIF4F complex in pachytene spermatocytes, thus promoting mRNA translation during spermatogenesis. Displays a high basal kinase activity. Acts as a mediator of the suppressive effects of IFNgamma on hematopoiesis. Negative regulator for signals that control generation of arsenic trioxide As(2)O(3)-dependent apoptosis and anti-leukemic responses. Involved in anti-apoptotic signaling in response to serum withdrawal (By similarity).[UniProtKB/Swiss-Prot Function]