Recql (NM_001012098) Rat Untagged Clone

CAT#: RN203532

Recql (untagged ORF) - Rat RecQ protein-like (DNA helicase Q1-like) (Recql), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Recql
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN203532 representing NM_001012098
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTCCATCCCAGCTCTAACGGATGAACTGGAGTCTGTGTCCAGTGAGCTCCATGCAGTTGACATCC
  AGATTCAAGAACTTACAGAGAGGCAGCACGAGCTCCTTCAGAGAAAGTCAGTCCTGACAAAGAGAATCAA
  GCAGTGCTTAGAGGACTCTGCTGCCGAGGCGAGCGGCGACTGTGACACGTCTCCAGCTGCATGGAGTAAA
  GAAGATTTCCCATGGTCCGGAAAGGTAAAACATGTGCTGCGAGATGTGTTTAAACTGCAGAAGTTCAGAC
  CTCTGCAGCTGGAGACTGTCAATGCCACCATGGCGAGAAAGGACATCTTTCTTGTCATGCCCACAGGAGG
  AGGGAAGAGCTTGTGCTACCAGCTGCCAGCGCTGTGCTCAGACGGTTTTACACTGGTGATTTGTCCACTC
  ATCTCTCTTATGGAAGATCAGCTGATGGTTTTACAACAGTTGGGGATTTCAGCTACTATGTTGAATTCTT
  CTAGCTCTAAGGAACATGTAAAATGTGTGCACACTGAAATGATGAATAAAAACTCCCACTTAAAGCTGAT
  TTATGTGACTCCAGAGAAAATTGCAAAAAGCAAGATGTTCATGTCGAGGCTAGAGAAAGCCTATGAGGCC
  GGGAGACTGACTGGAGTGGCGGTGGATGAAGTGCATTGCTGCAGTCAGTGGGGACATGACTTCAGGCCGG
  ATTACAAAGCACTCGGCATCTTGAAGCGCCAGTTTCCCAACATCTCACTGATTGGGCTGACAGCAACAGC
  AACCAACCATGTTTTGAAGGATGCACAGAAAATCCTATGTGTGGAGAAATGTTTGACATTTACAGCGTCT
  TTCAACAGACCAAATCTCTACTATGAGGTTCGGCAGAAGCCCTCGAGTGCTGAAGACTTTATCGAAAACA
  TTGCAAACCTTATTAATGGACGGTACAAAGGAAAGTCAGGAATCATATACTGTTTTTCTCAGAAAGACTC
  TGAACAAGTCACCATTAGCTTACAGAAGCTGGGGGTCCGTGCAGGCACTTACCATGCCAACATGGAGCCG
  GAGGACAGGACCAAGGTTCACACGCAGTGGTCAGCCAATGAGCTTCAGGTGGTAGTGGCAACAGTCGCAT
  TTGGCATGGGAATTGATAAGCCTGACGTGAGGTTTGTCATCCATCATTCAATGAGCAAATCCATGGAGAA
  TTACTACCAAGAGAGTGGGCGTGCCGGTCGAGATGACTGGAGAGCAGACTGTATTCTGTATTACGGCTTC
  GGAGACATATTCAGGATCAGCTCGATGGTGGTCATGGAGAATGTGGGACAGCAGAAGCTGTATGAGATGG
  TGTCATACTGCCAGAATATCAGCAAGTGTCGCCGTGCGTTAATAGCCCAACATTTTGATGAAGTGTGGAA
  TGCAGACGCCTGTAACAAAATGTGTGACAACTGCTGTAAAGACGACTCGTTTGAGAAAAAGAATATAACC
  GAGCACTGCCAAGCCCTGATCAAGATTCTGAAGCAGGCAGAAGGACTGAATGAAAAGCTCACCCCACTGA
  AGCTGATTGATGCTTGGATGGGAAAGGGAGCAGCCAAGTTCAGAGTGGCTGGCGTTGCTGTCCCAGCCCT
  TCCCCGAGAAGACCTGGAGAAAATCATCGTACATGCTCTCCTGCAGCAGTATCTCAAGGAAGACTACAGC
  TTCACGGCTTATGCTACCATCTCGTATCTGAAGGTGGGACCCAGAGCTAGTCTTCTTAGCAACGAAGGCC
  ATGCTGTCACTATGCAAGTGAAGAGGTCCACACAGAGCAGCGTTAGGGCTGCGTCACCTGAAGCTTGTGA
  AGTGGATTCAAAGGGGAAAGAAAAGAGCTCAGCAGTACTATGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001012098
• Insert Size: 1866 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001012098.1, NP_001012098.1
• RefSeq Size: 2427 bp
• RefSeq ORF: 1866 bp
• Locus ID: 312824
• UniProt ID: Q6AYJ1
• Cytogenetics: 4q44
• Gene Summary: DNA helicase that may play a role in the repair of DNA that is damaged by ultraviolet light or other mutagens. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction (By similarity).[UniProtKB/Swiss-Prot Function]