Lgsn (NM_181383) Rat Untagged Clone

CAT#: RN202990

Lgsn (untagged ORF) - Rat lengsin, lens protein with glutamine synthetase domain (Lgsn), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Lgsn
• Synonyms: Gluld1; Lgs
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN202990 representing NM_181383
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACGATGAAGGGGACCTTGCACAGGAGGACACAACAAAAGATGAAGCAAACTCGACTGAAGGCAGCA
  GGGTGAACAAGTTGAAAAGGACAAGAAGAAAAGTCACCAAACCACATCTTTGCTCAGCAGATGGGGATGA
  AATCACCATGGCCAATTCCAGGGAGATGTCCAGAAACCAAACAGCAGATCTGAGCAAACCTGGCTCTGCT
  GAGTCTTGGTCCTGGCACAATGCCAAAGACGCCCAAGATCAGATACCAGTGGTAAAATCTTCACTTCCCA
  GTGCTGGTGCTCCTGATGCTGAATTCAACCCGAATACTGATCACACTCGGGACAATGCTCAGAGTTTGAT
  CCTGCCTCAACTTTCTTCTAGAATGAAACACATCAAACAAGAAATGGCCAAAAATCACCTCCAGTTTGTA
  CGATTTGAAGCCACAGACTTGCACGGTGTCTCCAGATCTAAGAGCATCCCTGCACAATTTTTTCAAGAAA
  AAGTGATCCATGGAGTTTTCATGCCCCGTGGTTATCTGGAATTGATGCCGAACCCTAAGGACAATGAGGT
  AAATCACATACGAGCCACGTGCTTCAATAGTGACATAGTCCTCATGCCAGAGCTGTCGACCTTTAGAGTC
  TTGCCTTGGGCAGAGAGGACTGCGCGGGTGATTTGTGACACCTTCACTGTGACGGGCGAGCCTCTGCTGA
  CGTCCCCTAGGTACATCGCCAAGAGGCAGTTGCGCCAGCTGCAGGATGCCGGCTTCTCTCTGCTGTCTGC
  CTTCATCTATGATTTCTGCATTTTCGGTGTGCCCGAAGTGATCAATTCAAAGACCATATCTTTTCCTGCC
  TCGACATTACTAAGTAATCATGACCAGCCCTTCATGCAGGAGCTGGTTGACGGCTTGTATCACACAGGGG
  CAAATGTGGAGAGCTTCTCCTCTTCTACCAGGCCTGGGCAAATGGAGATCTGTTTTCTCCCGGAGTTTGG
  CATTAGCTCAGCGGATAATGCCTTCACCCTCAGAACAGGTGTCCAAGAAGTGGCCAGGAGATATAACTAC
  ATAGCAAGCCTTGTAATTGAGACTGGATTCTGCAACTCAGGAATTTTGTCTCATAGCATCTGGGATGTGA
  GCGGGAAGACAAACATGTTCTACAGTGGTTCTGGGGTGGAGAGGCTCACGCTCACCGGGAAGAAGTGGTT
  GGCAGGCCTGCTGAAGCACTCTGCGGCTCTCAGCTGCCTGATGGCCCCTGCTGTCAACTGCCGCAAACGC
  TACTGCAAGGACAGCAGAGACCTGAAGGACAGCGTGCCCACCACGTGGGGATACAACGACAACAGCTGTG
  CCTTGAACGTCAAGTGTCATGGTGAAAAAGGCACCCAGATAGAAAATAAATTAGGTTCGGCAACCGCGAA
  CCCTTACCTGGTATTGGCCGCTACTGTGGCAGCAGGCTTGGATGGTCTTCAGAGCAGTGATGGTGCTGCG
  GCTGAATCCGATGAGAGCCAGGATCTCTATCAACCAGAACCTTCCGAGATCCCTTTGAAGATGGAAGATG
  CCCTCGCCGCCCTAGAGCAAGATGAGTGTCTGAAGCAGGCACTGGGAGAGACTTTCATTCGCTATTTTGT
  TGCCATGAAGAAGTACGAACTGGAAAATGAAGAAACAGACGCAGAGGGAAATAAATTCCTGGAGTATTTT
  ATATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_181383
• Insert Size: 1686 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181383.2, NP_852048.1
• RefSeq Size: 1910 bp
• RefSeq ORF: 1686 bp
• Locus ID: 316304
• UniProt ID: Q7TT51
• Cytogenetics: 9q21
• Gene Summary: May act as a component of the cytoskeleton or as a chaperone for the reorganization of intermediate filament proteins during terminal differentiation in the lens. Does not seem to have enzymatic activity (By similarity).[UniProtKB/Swiss-Prot Function]