Casr (NM_016996) Rat Untagged Clone

CAT#: RN202906

Casr (untagged ORF) - Rat calcium-sensing receptor (Casr), (10 ug)

Product Images

Circular map for RN202906

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Casr
• Synonyms: PCaR1; RaKCaR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RN202906 representing NM_016996.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCATCGTACAGCTGCTGTTTGGCCCTCTTGGCTCTTGCCTGGCACTCCTCTGCCTATGGGCCTGAC
  CAGCGAGCCCAAAAGAAGGGGGACATTATCCTAGGAGGTCTCTTTCCTATCCATTTTGGAGTAGCAGCC
  AAAGATCAAGATCTGAAGTCAAGACCAGAGTCTGTGGAGTGCATTAGGTATAACTTCCGTGGATTCCGA
  TGGTTACAAGCCATGATATTCGCCATAGAGGAGATAAACAGCAGCCCCTCCCTTCTTCCCAACATGACA
  CTGGGATATAGGATATTTGACACCTGTAACACCGTCTCCAAGGCGCTGGAAGCCACCTTGAGTTTTGTT
  GCCCAGAACAAAATCGATTCTTTGAACCTGGACGAGTTCTGCAACTGCTCTGAGCACATCCCTTCGACC
  ATTGCCGTGGTGGGAGCCACCGGCTCCGGTGTCTCCACGGCGGTAGCCAACCTGCTGGGACTTTTCTAC
  ATCCCCCAGGTGAGCTACGCCTCCTCCAGCAGGCTCCTCAGCAATAAGAACCAGTACAAATCCTTCCTC
  CGCACCATTCCCAATGACGAACACCAGGCAACCGCGATGGCCGACATCATCGAGTACTTCCGCTGGAAC
  TGGGTGGGCACAATTGCAGCTGATGACGACTATGGCAGACCTGGCATTGAGAAGTTCCGAGAGGAAGCC
  GAAGAGAGGGATATCTGCATTGATTTTAGCGAGCTCATCTCCCAGTACTCTGACGAGGAAGAGATCCAG
  CAGGTGGTCGAAGTGATCCAAAACTCTACGGCCAAGGTCATTGTCGTTTTCTCCAGCGGCCCGGACCTA
  GAACCTCTCATCAAGGAGATTGTGCGGCGTAACATCACAGGCAGGATCTGGCTGGCTAGCGAGGCCTGG
  GCCAGTTCCTCGCTGATTGCTATGCCTGAGTATTTCCATGTAGTCGGGGGCACCATTGGGTTCGGTCTG
  AAGGCTGGGCAGATTCCAGGCTTCCGAGAATTCCTACAGAAAGTTCATCCTAGGAAGTCTGTCCACAAT
  GGTTTTGCCAAAGAGTTTTGGGAAGAAACTTTTAATTGCCACCTCCAAGAAGGCGCAAAAGGACCTTTA
  CCTGTGGACACCTTCGTGAGAAGTCACGAAGAAGGTGGCAACAGGTTACTCAATAGCTCTACTGCCTTC
  CGACCCCTCTGCACAGGGGATGAGAACATCAACAGTGTGGAGACCCCTTACATGGATTACGAACATTTA
  CGGATATCCTACAATGTGTACTTAGCCGTCTACTCCATTGCGCATGCCCTACAAGATATATACACCTGC
  TTACCCGGAAGAGGGCTTTTCACCAACGGGTCCTGTGCAGACATCAAGAAGGTTGAGGCCTGGCAGGTC
  TTGAAGCACCTACGGCACCTGAACTTCACCAACAACATGGGGGAGCAGGTGACCTTCGATGAGTGTGGT
  GATCTGGTGGGGAACTATTCTATCATCAACTGGCACCTCTCCCCAGAGGACGGCTCCATTGTGTTCAAG
  GAAGTTGGGTACTACAATGTGTATGCCAAGAAGGGAGAAAGACTCTTCATCAATGAGGAGAAGATCTTG
  TGGAGTGGGTTCTCCAGAGAGGTGCCTTTCTCCAATTGCAGCCGGGACTGTCAGGCAGGGACCAGGAAG
  GGGATCATCGAGGGAGAGCCCACCTGCTGCTTTGAGTGTGTGGAGTGTCCTGATGGAGAGTACAGTGGA
  GAGACAGATGCGAGTGCCTGTGACAAGTGCCCGGATGACTTCTGGTCCAATGAGAACCACACTTCTTGC
  ATTGCCAAGGAGATTGAGTTTCTGGCGTGGACCGAGCCCTTTGGAATCGCTCTCACTCTCTTTGCGGTG
  CTGGGCATTTTCCTGACCGCCTTTGTGCTGGGTGTCTTCATCAAGTTCCGAAACACACCTATCGTCAAG
  GCCACCAACCGAGAACTGTCCTACCTCCTGCTCTTCTCCCTACTCTGCTGCTTCTCCAGCTCCTTGTTC
  TTCATTGGGGAGCCCCAGGACTGGACGTGCCGCCTGCGACAGCCTGCTTTCGGCATCAGCTTTGTGCTC
  TGTATCTCGTGCATCTTGGTGAAGACCAATCGCGTCCTCCTGGTATTTGAAGCCAAGATACCCACCAGC
  TTCCACCGGAAGTGGTGGGGGCTCAACCTGCAGTTCCTGCTGGTTTTCCTCTGCACCTTCATGCAGATC
  CTCATCTGCATCATCTGGCTCTACACGGCGCCCCCCTCTAGCTACCGCAACCATGAGCTGGAAGACGAA
  ATCATCTTCATCACGTGCCATGAGGGCTCACTCATGGCACTTGGCTCCCTGATCGGCTATACCTGCCTG
  CTGGCTGCCATCTGCTTCTTCTTTGCCTTCAAGTCCAGGAAGTTACCAGAGAACTTCAACGAAGCCAAG
  TTCATTACCTTCAGCATGCTCATCTTCTTCATCGTCTGGATCTCCTTCATTCCAGCCTATGCCAGCACC
  TACGGCAAGTTTGTCTCTGCCGTAGAGGTGATCGCCATTTTGGCAGCCAGCTTTGGCTTACTAGCCTGC
  ATCTTCTTCAACAAGGTCTACATTATCCTCTTCAAGCCTTCCCGGAACACCATTGAGGAAGTCCGCTCC
  AGCACCGCAGCACATGCTTTCAAAGTAGCAGCCCGCGCCACTCTACGCCGTCCCAACATCTCCCGGAAG
  CGGTCCAGCAGCCTTGGAGGCTCCACCGGCTCCATTCCCTCCTCCTCCATCAGCAGCAAAAGCAACAGC
  GAAGACCGGTTCCCGCAGCCAGAGAGGCAGAAGCAACAGCAACCGCTGTCCCTGACCCAGCAAGAACAG
  CAGCAGCAGCCCCTGACCCTCCACCCACAGCAACAGCAGCAGCCACAGCAGCCGAGATGCAAACAGAAG
  GTCATCTTCGGCAGTGGTACGGTCACCTTCTCTCTGAGTTTTGACGAGCCTCAGAAGAATGCCATGGCC
  CACAGGAACTCCATGCGTCAGAACTCCCTGGAGGCCCAGAGGAGCAACGACACCTTGGGCAGACACCAG
  GCCCTGCTTCCCCTACAGTGTGCAGATGCGGACTCAGAAATGACCATTCAGGAAACGGGCCTGCAAGGG
  CCCATGGTGGGGGACCACCAGCCAGAAATGGAAAGCTCAGATGAAATGTCCCCAGCGCTGGTCATGTCC
  ACCTCTCGGAGCTTCGTCATTAGTGGTGGAGGTAGCTCTGTGACGGAAAACGTATTACACTCCTAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
  Protein Sequence

  MASYSCCLALLALAWHSSAYGPDQRAQKKGDIILGGLFPIHFGVAAKDQDLKSRPESVECIRYNFRGFR
  WLQAMIFAIEEINSSPSLLPNMTLGYRIFDTCNTVSKALEATLSFVAQNKIDSLNLDEFCNCSEHIPST
  IAVVGATGSGVSTAVANLLGLFYIPQVSYASSSRLLSNKNQYKSFLRTIPNDEHQATAMADIIEYFRWN
  WVGTIAADDDYGRPGIEKFREEAEERDICIDFSELISQYSDEEEIQQVVEVIQNSTAKVIVVFSSGPDL
  EPLIKEIVRRNITGRIWLASEAWASSSLIAMPEYFHVVGGTIGFGLKAGQIPGFREFLQKVHPRKSVHN
  GFAKEFWEETFNCHLQEGAKGPLPVDTFVRSHEEGGNRLLNSSTAFRPLCTGDENINSVETPYMDYEHL
  RISYNVYLAVYSIAHALQDIYTCLPGRGLFTNGSCADIKKVEAWQVLKHLRHLNFTNNMGEQVTFDECG
  DLVGNYSIINWHLSPEDGSIVFKEVGYYNVYAKKGERLFINEEKILWSGFSREVPFSNCSRDCQAGTRK
  GIIEGEPTCCFECVECPDGEYSGETDASACDKCPDDFWSNENHTSCIAKEIEFLAWTEPFGIALTLFAV
  LGIFLTAFVLGVFIKFRNTPIVKATNRELSYLLLFSLLCCFSSSLFFIGEPQDWTCRLRQPAFGISFVL
  CISCILVKTNRVLLVFEAKIPTSFHRKWWGLNLQFLLVFLCTFMQILICIIWLYTAPPSSYRNHELEDE
  IIFITCHEGSLMALGSLIGYTCLLAAICFFFAFKSRKLPENFNEAKFITFSMLIFFIVWISFIPAYAST
  YGKFVSAVEVIAILAASFGLLACIFFNKVYIILFKPSRNTIEEVRSSTAAHAFKVAARATLRRPNISRK
  RSSSLGGSTGSIPSSSISSKSNSEDRFPQPERQKQQQPLSLTQQEQQQQPLTLHPQQQQQPQQPRCKQK
  VIFGSGTVTFSLSFDEPQKNAMAHRNSMRQNSLEAQRSNDTLGRHQALLPLQCADADSEMTIQETGLQG
  PMVGDHQPEMESSDEMSPALVMSTSRSFVISGGGSSVTENVLHS*
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
  Fully Sequenced ORF

  >MC208090 representing NM_194350
  Red=Cloning site Blue=ORF
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGCGGAGCTGGCGATGGGCGCAGAGCTGCCCAGCAGCCCACTGGCCATCGAGTACGTCAACGACT
  TCGACCTGATGAAGTTCGAGGTGAAGAAGGAGCCGCCCGAGGCCGAGCGCTTCTGCCACCGCCTGCCGCC
  CGGCTCGCTGTCCTCGACGCCCCTCAGCACGCCCTGCTCCTCGGTGCCCTCTTCGCCCAGCTTCTGCGCA
  CCCAGCCCGGGCACAGGCGGCGGCGCGGGCGGCGGGGGCAGCGCGGCTCAGGCCGGGGGCGCCCCGGGGC
  CGCCGAGTGGAGGCCCCGGCACTGTCGGGGGCGCCTCAGGAAAAGCGGTGCTGGAGGATCTGTACTGGAT
  GAGCGGGTACCAGCACCACCTGAACCCCGAGGCGCTCAACCTGACGCCGGAGGACGCGGTGGAGGCGCTC
  ATCGGCAGCGGCCACCACGGCGCGCACCACGGCGCGCATCACCCGGCGGCTGCTGCGGCCTATGAGGCCT
  TCCGGGGTCAGAGCTTCGCGGGCGGCGGCGGCGCGGACGACATGGGTGCCGGCCACCACCACGGCGCACA
  CCACACTGCCCACCATCATCACTCTGCCCACCATCACCATCACCACCATCACCACCACGGAGGCTCTGGC
  CACCACGGCGGAGGCGCGGGTCACGGCGGAGGCGGCGCAGGCCACCACGTGCGCTTGGAGGAGCGCTTCT
  CCGACGACCAGCTGGTATCCATGTCCGTGCGGGAGCTGAACCGGCAGCTCCGCGGCTTCAGCAAGGAGGA
  GGTCATCCGACTGAAACAGAAGCGGCGCACGCTCAAGAACCGCGGCTACGCGCAGTCGTGCCGCTTCAAG
  CGGGTGCAGCAGCGGCACATTCTGGAGAGCGAGAAGTGCCAGCTCCAGAGCCAGGTGGAGCAGCTGAAGC
  TGGAGGTGGGGCGTCTGGCCAAGGAGCGGGACCTGTACAAGGAGAAATACGAGAAGTTGGCGGGCCGGGG
  CGGCCCCGGGGGCGCGGGCGGGGCCGGCTTCCCTCGGGAGCCCTCGCCAGCGCAGGCTGGCCCCGGGGCG
  GCCAAAGGCGCACCCGACTTCTTTCTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI Plasmid Map
• ACCN: NM_016996
• Insert Size: 3240 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016996.1
• RefSeq Size: 4113 bp
• RefSeq ORF: 3240 bp
• Locus ID: 24247
• UniProt ID: P48442
• Cytogenetics: 11q22
• MW: 120.9 kDa
• Gene Summary: a G protein-coupled receptor that senses extracellular calcium; involved in regulating the secretion of parathyroid hormone, bile and intestinal fluid [RGD, Feb 2006]
  Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.