Wnt10b (NM_001108111) Rat Untagged Clone

CAT#: RN202816

Wnt10b (untagged ORF) - Rat wingless-type MMTV integration site family, member 10B (Wnt10b), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Wnt10b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN202816 representing NM_001108111
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGAGGAGCCCCGGTCTCGGCCTCCGCCCTTAGGCCTCGCGGGTCTCCTGTTCTTGGCTTTGTTCA
  GTCGGGCTCTAAGCAATGAGATTCTGGGCCTAAAACTTCCCGGTGAGCCGCCGCTGACGGCCAACACCGT
  GTGCTTGACCCTGTCCGGCTTGAGTAAGCGGCAGCTGGGGCTGTGCCTGCGCAGCCCCGACGTGACGGCG
  TCCGCGCTCCAGGGGCTGCACATCGCCGTTCACGAGTGTCAGCACCAGCTGCGCGACCAGCGCTGGAACT
  GCTCGGCACTAGAGGGTGGCGGCCGGCTGCCGCACCACAGCGCCATCCTCAAGCGCGGTTTCCGTGAGAG
  TGCGTTCTCCTTCTCCATGCTGGCTGCTGGTGTCATGCACGCAGTTGCCACAGCCTGCAGCTTGGGTAAG
  CTGGTGAGCTGTGGCTGCGGCTGGAAGGGCAGCGGTGAGCAGGACCGGCTTCGGGCCAAGTTGTTGCAGC
  TTCAGGCACTGTCTAGGGGCAAGAGTTTCCCCCACTCCCAGCCCAGCCCTATTCCTGGCTCTGTCCCCGG
  TCCTGGCCCCCAGGACACGTGGGAATGGGGTGGCTGTAATCACGACATGGACTTTGGAGAGAAGTTCTCT
  CGGGATTTCTTGGATTCCAGGGAAGCTCCCCGGGACATCCAGGCGAGAATGCGGATCCACAACAACAGGG
  TGGGGCGCCAGGTGGTAACCGAAAACCTGAAGCGGAAGTGCAAATGCCATGGCACATCAGGCAGCTGCCA
  ATTCAAGACCTGTTGGAGGGCAGCCCCAGAGTTCCGGGCCATCGGGGCAGCACTGAGGGAGCGGTTGGGC
  AGAGCCATCTTCATCGATACCCATAACCGCAATTCTGGAGTTTTCCAGCCCCGCCTACGTCCGCGTCGTC
  TCTCAGGGGAGCTGGTTTACTTTGAGAAGTCTCCTGACTTCTGTGAGCGAGACCCCGCTTTGGGCTCCCC
  AGGCACGAGAGGCCGGGCTTGCAACAAGACCAGCCGCCTATTGGATGGCTGTGGTAGCCTGTGCTGTGGC
  CGTGGGCACAACGTGCTCCGGCAGACGCGAGTGGAGCGCTGCCACTGTCGTTTCCACTGGTGCTGTTACG
  TGCTGTGTGATGAGTGTAAAGTCACAGAGTGGGTCAACGTGTGTAAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001108111
• Insert Size: 1170 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001108111.1, NP_001101581.1
• RefSeq Size: 2234 bp
• RefSeq ORF: 1170 bp
• Locus ID: 315294
• Cytogenetics: 7q36