Parg (NM_031339) Rat Untagged Clone

CAT#: RN202387

Parg (untagged ORF) - Rat poly (ADP-ribose) glycohydrolase (Parg), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Parg
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN202387 representing NM_031339
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGCGGGCCCCGGCTGTGAGCCCTGCACGAAGCGGCCCCGCTGGGGTGCCGCTGGAACTTCTGCGC
  CGACTGCCTCGGACTCCCGGAGCTTCCCTGGCAGGCAGAAGCGCGTTCTCGACCCTAAGGACGCTCCCGT
  ACAGTTCAGGGTCCCACCGTCCTCGTCAGCCTGCGTCTCGGGGCGGGCGGGACCGCACAGAGGCAGCGTC
  ACCTCGTTTGTTTTCAAACAAAAGCCTATTACCACTTGGATGGATACTAAAGGACCCAAGACAGCTGAAT
  CAGAAAGTAAAGAAAACAACAATACCAGGACTGACCCCATGATGAGTTCTGTGCAGAAAGATAACTTTTA
  CCCACATAAGGTAGAGAAATTGGGAAATGTTCCTCAACTAAATCTTGATAAATCACCCACTGAGAAGAGC
  ACGCCGTATTTGAACCAACAGCAGACTGCAGGCGTGTGCAAGTGGCACAGTGCAGGGGAGCGTGCAGAGC
  AGCTCTCGGCCAGCGAGCCTTCAGCAGTGACTCAGGCACCAAAGCAGCTTAGTAATGCGAACATTGATCA
  GTCACCCCCAACTGATGGCCACAGTGACACCGATCATGAGGAAGACAGAGACAATCAACAGTTCCTTACG
  CCCGTGAAGCTTGCAAATGCAAAGCAAACAGTAGGGGATGGGCAAGCCAGGAGCAACTGTAAGTGCAGTG
  CGTCTTGTCAGTGTGGGCAAGACTGTGCAGGCTGTCAGCGGGAGGAGGCCGATGTCATACCAGAGAGTCC
  TTTGTCAGATGTTGGCGCTGAGGACATTGGAACTGGATCAAAAAATGACAACAAGTTGACTGGACAAGAA
  AGTGGCCTCGGTGATTCACCTCCGTTTGAGAAAGAAAGTGAGCCTGAGTCACCAATGGATGTAGACAACT
  CGAAAACCAGTTGTCAAGATTCAGAAGCAGATGAAGAAGCAAGTCCAGTCTTTGATGAGCAAGACGACCA
  AGACGACCGCTCTTCCCAGACAGCAAATAAACTTTCAAGTCGCCAAGCAAGAGAAGTTGACGGCGATCTT
  AGGAAACGCTATTTGACTAAGGGAAGTGAAATTAGATTGCATTTCCAGTTTGAAGGAGGGAGTAATGCTG
  GGACCAGTGATTTAAATGCCAAGCCATCTGGAAACTCTTCTAGCCTTAATGTAGACGGTAGAAGTTCCAA
  GCAGCATGGAAAAAGGGATTCTAAAATTACAGATCATTTTGTGAGAATTCCCAAGTCAGAGGACAAAAGA
  AAAGAACAATGTGAAGTCAGACATCAAAGAGCAGAAAGGAAGATCCCTAAATACGTTCCACCTAACCTTC
  CTCCAGATAAGAAGTGGTTGGGAACTCCTATTGAGGAAATGAGAAAAATGCCTCGGTGTGGGGTCCGCCT
  GCCTCTCTTAAGACCGTCTGCAAGCCACACAGTAACTGTCCGGGTAGACCTTCTGAGAGCAGGAGAGGTT
  CCAAAACCGTTTCCAACACATTACAAAGATTTGTGGGATAATAAGCACGTTAAAATGCCTTGTTCAGAAC
  AAAACTTGTACCCTGTGGAAGATGAGAATGGTGAGCGAACTGCAGGGAGTAGGTGGGAGCTCATTCAGAC
  CGCACTCCTCAACAAATTCACGCGACCCCAGAACCTGAAGGATGCTATTCTGAAGTACAATGTGGCATAT
  TCTAAGAAATGGGACTTTACAGCTTTGGTTGATTTCTGGGATAAGGTACTTGAAGAAGCAGAAGCCCAAC
  ATTTGTATCAGTCCATTTTACCCGACATGGTGAAAATTGCACTCTGTCTGCCAAATATTTGCACCCAGCC
  AATACCACTTCTGAAACAGAAGATGAACCATTCTGTCACGATGTCACAGGAACAGATCGCCAGTCTTCTG
  GCTAATGCTTTCTTCTGCACATTCCCCCGACGGAATGCCAAGATGAAATCGGAGTATTCTAGTTACCCAG
  ACATTAACTTCAATCGGTTGTTTGAAGGACGTTCATCAAGGAAACCAGAGAAACTGAAAACACTCTTCTG
  CTACTTTCGAAGAGTCACAGAGAAAAAACCTACTGGATTGGTGACGTTTACAAGACAGAGTCTTGAAGAT
  TTTCCAGAATGGGAAAGATGTGACAAACCTCTGACACGCTTACATGTCACTTACGAGGGTACCATAGAAG
  GCAACGGCCGAGGCATGCTCCAGGTGGATTTTGCGAATCGTTTTGTTGGAGGTGGTGTAACTGGTGCAGG
  ACTTGTACAAGAAGAAATCCGATTTTTAATCAATCCTGAGTTGATTGTTTCACGGCTCTTCACTGAGGTG
  CTGGATCACAATGAATGTCTTATTATCACAGGTACTGAACAGTACAGTGAATACACGGGCTATGCTGAAA
  CTTATCGTTGGGCCCGGAGCCACGAAGATGGGAGTGAGAAGGATGATTGGCAGCGATGCTGCACGGAGAT
  CGTCGCCATTGATGCACTTCACTTCAGACGCTACCTCGATCAGTTTGTGCCTGAGAAAGTGAGACGTGAG
  CTTAACAAGGCTTACTGTGGATTCCTCCGTCCTGGCGTTCCTCCTGAGAACCTCTCTGCAGTGGCCACAG
  GAAACTGGGGCTGTGGTGCCTTTGGGGGCGATGCTAGATTAAAAGCCTTAATACAGTTACTGGCCGCTGC
  TGCCGCTGAACGCGATGTGGTTTATTTCACTTTCGGGGACTCAGAGTTGATGAGAGACATTTACAGCATG
  CACACTTTCCTCACTGAGAGGAAACTGAATGTCGGGAAAGTGTACAGGTTACTGCTTAGATACTACAGGG
  AAGAGTGCAGAGACTGCTCCAGCCCTGGACCAGACACCAAGCTCTATCCATTCATATACCATGCTGCCGA
  GTCCAGTGCAGAGACCTCCGACCAGCCAGGACAGAGGACGGGCACCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_031339
• Insert Size: 2919 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_031339.1, NP_112629.1
• RefSeq Size: 3974 bp
• RefSeq ORF: 2919 bp
• Locus ID: 83507
• UniProt ID: Q9QYM2
• Cytogenetics: 16p16
• Gene Summary: catalyzes the process of poly(ADP-ribosyl)ation of proteins; plays a role in telomere maintenance and suppressing recombination atDNA ends [RGD, Feb 2006]