Pip5k1b (NM_001012743) Rat Untagged Clone

CAT#: RN200784

Pip5k1b (untagged ORF) - Rat phosphatidylinositol-4-phosphate 5-kinase, type I, beta (Pip5k1b), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Pip5k1b
• Synonyms: Pip5k1a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN200784 representing NM_001012743
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGTCAACTGCTGAAAATGGAGACGCCGTACCTGGAAAACAAAACGAAGAAAAAACCTACAAAAAGA
  CTGCATCATCAGCTATTAAAGGTGCCATTCAGCTGGGAATAGGATACACAGTGGGTAATCTCACTTCCAA
  GCCAGAACGCGATGTCCTCATGCAAGACTTTTATGTGGTGGAAAGTGTATTCCTCCCCAGTGAAGGGAGC
  AATCTGACCCCAGCACATCACTACCCAGACTTCAGGTTTAAGACATACGCCCCGCTGGCTTTCCGCTACT
  TCCGAGAACTCTTTGGTATCAAGCCCGACGATTACTTGTACTCCATCTGCAGTGAACCGCTGATAGAACT
  GTCCAATCCTGGAGCCAGTGGATCCTTGTTTTTCTTGACCAGCGATGATGAATTTATTATCAAAACTGTT
  CAGCATAAGGAAGCCGAGTTCCTGCAGAAGCTGCTGCCAGGCTATTACATGAATCTAAACCAGAATCCAA
  GGACTCTCCTGCCGAAATTCTATGGTCTGTATTGCATGCAGTCAGGAGGCATCAACATCCGCATCGTGGT
  GATGAACAACGTGCTGCCGCGTGCCATGAGGATGCACTTGACCTATGACCTGAAAGGCTCCACGTACAAA
  CGAAGGGCGTCCCGAAAAGAGAGGGAGAAACCCAACCCCACGTTTAAGGACCTGGACTTCCTGCAGGACA
  TGCATGAGGGGCTGTATTTCGATACAGAAACATACAATGCCCTTATGAAGACACTACAGAGAGACTGCCG
  GGTGCTGGAAAGCTTCAAGATCATGGACTACAGCCTTTTGCTGGGTATCCACATCTTGGACCATTCCCTC
  AAAGACAAAGAAGAGGAGCCCCTCCAAAATGCACCCGACGCAAAGCGGCCGGGGATGCAGAAAGTCCTCT
  ACTCCACAGCCATGGAGTCCATCCAGGGTCCAGGCAAGTCCGCAGATGGGATCATTGCAGAGAACCCAGA
  CACAATGGGAGGCATTCCAGCTAAAAGCCATAAGGGAGAAAAGCTGCTTCTATTCATGGGCATTATTGAT
  ATTCTCCAATCATATAGGTTAATGAAGAAGTTAGAACACTCCTGGAAAGCTCTTGTGTATGATGGGGACA
  CCGTTTCAGTTCATAGACCAAGTTTTTATGCAGATAGATTTTTAAAGTTTATGAATTCCAGAGTTTTCAA
  GAAAATTCAAGCTCTGAAGGCCTCACCTTCTAAGAAACGGTGCAACTCCATCGCTGCACTGAAGGCAACC
  TCCCAGGAGATTCTGTCCTCCATCAGCCAAGAATGGAAGGATGAGAAGCAGGATCTGTTAACCGAAGGGC
  AGAGTTTCAGCAGCCTGGATGAAGAAGCCCTGGGATCCCGACACAGGCCTGACCTGGTGCCCAGCACGCC
  GTCATTATTTGAAGCTGCTTCCTTAGCAACCACAATATCATCTTCTTCCTTATATGTCGGTGAGCACTAT
  CCACACGACAGGACTACGCTTTACTCAAACAGCAAAGGGTTACCTTCCAGTTCAACCTTCACCTTGGAAG
  AGGGGACCATCTACCTGACTGCTGAGCCAAACGCCCTGGAAACGCAGGATGACGCCTCCGTGCTGGATGT
  CTATTTATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001012743
• Insert Size: 1620 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001012743.1, NP_001012761.1
• RefSeq Size: 2915 bp
• RefSeq ORF: 1620 bp
• Locus ID: 309419
• UniProt ID: Q5CZZ9
• Cytogenetics: 1q51
• Gene Summary: Participates in the biosynthesis of phosphatidylinositol 4,5-bisphosphate. Mediates RAC1-dependent reorganization of actin filaments. Contributes to the activation of PLD2. Together with PIP5K1A is required after stimulation of G-protein coupled receptors for stable platelet adhesion (By similarity).[UniProtKB/Swiss-Prot Function]