Lsg1 (NM_001013421) Rat Untagged Clone

CAT#: RN200747

Lsg1 (untagged ORF) - Rat large subunit GTPase 1 homolog (S. cerevisiae) (Lsg1), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Lsg1
• Synonyms: RGD1309089
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN200747 representing NM_001013421
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCCGGAGAAGAGCGCCGGGTGGTGGGTCACTGGGTCGGGTACTTATCCGGCATCAGACTCAACGAA
  GCCGCAGTCACCGTCATACTGACTCCTGGCTGCATACGAGTGAACTCAATGATGGTTATGATTGGGGACG
  TCTGAATCTTCAGTCAGTGACGGAACAGAGCTCCCTCGAGGACTTTCTTGCAACTGCAGAACTTGCAGGG
  ACGGAGTTCGTGGCTGAGAAGCTTAATATTAAGTTTGTGCCCCCTGAGGCTAGAACTGGACTGCTGTCTT
  TCGAGGAGAGCCAGAGAATTAAGAGACTCCATGAAGAAAACAGACAGTTCCTGTGTATACCAAGGAGACC
  AAATTGGGACAGAAAAACTAGCCCTGAAGAACTGAAACAAGCAGAGAAAGATAACTTTCTAAAATGGAGG
  CGCCAGCTTGTCCGGCTAGAAGAAGAACAGAAGTTGATATTGACTCCCTTTGAACGGAATTTGGACTTCT
  GGCGCCAGCTCTGGAGGGTCATTGAGAGAAGTGATATTGTGGTCCAGATAGTAGATGCTCGGAACCCGCT
  CCTGTTCAGATGTGAGGATTTGGAATGTTACGTGAAGGAGATAGATGCTGCCAAGGAGAATGTGATTCTG
  ATAAACAAGGCAGACTTGTTGACTGCTGAGCAGAGGGTTGCCTGGGCTGTGCACTTTGAGAAAGAAGGTG
  TGAAGGTTATCTTCTGGTCAGCTTTGGCAGAAACCGTTCACCTAAATGGTGACTCTAAGGATGAAGTAAA
  CAGTGTTGCTGGAGAAGCCAACAGCTCTGAGTCTGAAGACTCCAGTTTGGACGGGAATGAAATTCCACAC
  AGAGACTTGTTCTTGCTTAGTGAGGAGTCCGAAAGTGACGATGACGACAGTGAGTATGAGGACTGTCAGG
  AGGACGAGGAGGAAGATTGGCAGACGTGTTCTGAAGAAGATAGCAATCCTGAGGAGGGGCAGGAGGAAGG
  GGGCTGTGATCGGGACCAGAAGGAACACGGTCCTGAGGATTCTGAGGCTCAGAGCAGAGCATCACCAGAA
  AATTCACAGATGAGCAACAAGAGCCATCTGGTCTCGAAGCAGGAGTTACTAGAGCTCTTCAAGAAACTCC
  ACACTGGGAAGAAGGTGAAAGACGGACAGCTGACTGTAGGGCTGGTGGGCTACCCTAATGTTGGTAAGAG
  CTCAACCATCAACACCATCATGGGCAACAAGAAAGTATCGGTGTCTGCCACGCCAGGCCACACCAAGCAT
  TTCCAGACCCTGTACGTGGAGCCAGGCCTCTGTCTGTGTGACTGCCCCGGCCTGGTGATGCCATCTTTTG
  TGTCTACCAAAGCAGAGATGATTTGCAGTGGGATCCTCCCTATCGACCAGATGAGAGATCACGTTCCGCC
  TGTATCACTAGTTTGCCAGAATATCCCGAGGCGAGTTTTAGAAGCTACCTATGGCATTAACATTATAAAG
  CCTGGAGAAGATGAAGACCCCTACCGACCTCCGACCTCAGAAGAGCTGTTGACAGCTTATGGATGCATGC
  GGGGATTCATGACAGCACACGGACAGCCAGACCAGCCTCGGTCTGCACGATACATCCTGAAGGACTACGT
  CAGAGGTAAACTTCTGTATTGTCACCCTCCTCCTGGAAAAGACCCTGTGGCATTCCAGCATCAACACAGG
  CAACTCCTAGAGAACAAAATAAAAGGTGAAGAACTGAGGCTTCAGCCAGGCAAAACCCAAAAAGCCAAAC
  AGGTTGAAAATGTAGTCGACAAGACTTTTTTCCATCAGGAGAATGTCAGAGCATTGACCAAAGGGGTCCA
  GGCTGTCATGGGTTACAAGCCCGGGAGTGGCCTGGTGACTGCAGCTGCTGCGAGTGCTGAGAACGTACCT
  GGGAAGCCCTGGAAAAAACACGGTAACAGAAACAAAAAAGAGAAAAGCCGCAGGCTCTACAGGCACCTGG
  ATGTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001013421
• Insert Size: 1968 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_001013421.1, NP_001013439.1
• RefSeq Size: 2283 bp
• RefSeq ORF: 1968 bp
• Locus ID: 288029
• UniProt ID: Q5BJT6
• Cytogenetics: 11q22
• Gene Summary: GTPase required for the XPO1/CRM1-mediated nuclear export of the 60S ribosomal subunit. Probably acts by mediating the release of NMD3 from the 60S ribosomal subunit after export into the cytoplasm (By similarity).[UniProtKB/Swiss-Prot Function]