Cast (NM_053295) Rat Untagged Clone

CAT#: RN200416

Cast (untagged ORF) - Rat calpastatin (Cast), transcript variant 1, (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Cast
• Synonyms: MGC108997
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN200416 representing NM_053295
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCGGCCCGGCCCGAAGCCCGCCGCCTCTTCCAGGCCCCGGCGCGGGGCGGCAGCTAGCCACACCC
  AGGAGCATGTTAATGAAAAGAATATTGGATCATCTTCTAAACCAGCAGAAAAGAAAGGATCTGATGAGGT
  GACAGCTTCCTCTGCTGCCACTGGCACATCTCCCAGAATGAGTACCACAGGAGCTAAGGCTGTGAAAATA
  GAGTCTGAGAAGTCACAATCAAGTGAGCCACCTGTGATTCATGAGAAAAAGCCAAAAGGAAAGCCAAAGG
  AAGGGTCAGAGCCCCAAACTCTTCCTAAGCATGCATCAGATACAGGAAGTAAGCATGCTCACAAAGAAAA
  AGCACTTTCCAGATCAAATGAGCAGATAGTGTCAGAGAAATCATCAGAATCAAAGACAAAATTTCAAGAT
  GCTCCTTCTGCTGATGGAGAGAGTGTGGCTGGAGGTGTGACTGTGGCCACTGCCTCAGACAAGGTGGTTG
  TTAAGAAAAAAGAGAAGAAATCATTAACACCTACTTTACCTATGGAGTCCACACTAAACAAGCTATCTGA
  CAAGTCAGGCGTGAATGCTGCTTTGGATGACCTGATAGACACCTTAGGTGAATGTGAAGATACTAACAAA
  GATGATCCACCATATACTGGACCAGTAGTTCTGGACCCAATGGATTCTACCTACCTTGAGGCACTGGGTA
  TAAAAGAAGGGACTATTCCTCCAGAGTATAGGAAACTTCTGGAGAAAAATGAAGCTATCACAGGACCTCT
  TCCAGATTCTCCTAAACCTATGGGAATCGACCATGCTATAGATGCCTTGTCATCTGATTTCACCTGTAGC
  TCTCCAACTGGAAAGCAAACTGAGAAAGAGAAGTCTACTGGGGAAAGTTCGAAAGCTCAGTCTGCAGGAG
  TGACCAGAAGTGCTGTTCCTCCCCAGGAGAAGAAAAGGAAAGTAGAAGAGGAGGTCATGAATGATCAAGC
  CCTGCAGGCTCTGTCAGATTCACTGGGCACCCGGCAGCCAGACCCTCAGAGCCATCTTAGGCAAGCCAAA
  CAAGTCAAAGAGGCAAAAGCAAAAGAAGAAAGGCAGGAGAAATGTGGTGAAGATGAGGACACAGTACCTG
  CTGAGTACAGGTTAAAACCAGCCAAGGATAAAGATGGAAAACCACTATTGCCAGAACCTGAAGAAACATC
  TAAGTGCCTGAGTGAGTCAGAGCTGATTGGGGAGCTTTCAGCAGATTTTGTCCAACCTACATATCAAGAA
  AAACCATCTATGCCAGCTGCAAAAATAAAGAAGGGCGTAGTGCCAGATGATGCTGTAGAAACCTTGGCTA
  GAAGCCTGGGGACAAGGAAAGAAGATCCAGAAGATGAAAAATCTCTGGTGGATAAAGTCAAGGAGAAAGC
  TAAAGAAGAAGATCATGAAAAACTTGGTGAAAAAGAAGAAACAATTCCTCCTGATTATCGACTAGAAATA
  GTCAAGGATAAGGATGGAAAACCGCTCCTGCCAAAAGAAGCCGAGGAACAACTTCCACCCTTAAGCGATG
  ACTTCCTTCTTGATGCATTGTCTCAGGACTTCTCCAGTCCTGCAAACATATTGTCTCTTGGTTTTGAAGA
  TGCCAAACTTTCTGCTGCTGTTTCTGAAACAGTTTCCCAGGTGCCTGCTCCAAGCAACCACACAGCAGCC
  CCACCGCCTGGCACTGAGAGGAGAGACAAAGAACTCGATGATGCCTTGGATGAACTTTCTGACAGTCTTG
  GACAAAGACAGCCTGATCCAGATGAGAACAAACCACTAGATGATAAAGTAAAGGAAAAAATCAAAGCAGA
  GCATAGCGAGAAACTGGGAGAAAGAGACGACACCATCCCCCCTGAATATAGGCATCTCCTGGATAATGAT
  GGGAAGGACAAACCAGAGAAGCCACTGACAAAGAACACAGAGAAGCCTGGTCAGGACCAGGACCCCATTG
  ATGCCCTCTCAGAAGACTTGGATAGCTGCCCCCCAACTACAGAGACTTCACAGAATACAACAAAGGAGAA
  GGGTAAGAAGACTTCAAGTTCCAAAGCATCCAAGAATGAAGAGAAAACAAAGGATTCCTCCAAGAAGACA
  GAGGAAGTGCCCAAGCCAAAGGTGGATGAAGATGCAACATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_053295
• Insert Size: 2142 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053295.2, NP_445747.2
• RefSeq Size: 4649 bp
• RefSeq ORF: 2142 bp
• Locus ID: 25403
• UniProt ID: P27321
• Cytogenetics: 2q11
• Gene Summary: inhibitor of calpain; degradation is required for myoblast fusion; exists in multiple alternatively spliced isoforms [RGD, Feb 2006]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a).