XRN1 (NM_001282857) Human Tagged ORF Clone

CAT#: RG240201

XRN1 (tGFP-tagged) - Human 5'-3' exoribonuclease 1 (XRN1), transcript variant 3

Product Images

Circular map for RG240201

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: XRN1
• Synonyms: SEP1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG240201 representing NM_001282857.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGGAGTCCCCAAGTTTTACAGATGGATCTCAGAGCGGTATCCCTGTCTCAGCGAAGTGGTGAAAGAG
  CATCAGATTCCTGAATTTGACAACTTGTACCTGGATATGAATGGAATTATACATCAGTGCTCCCATCCT
  AATGATGATGATGTTCACTTTAGAATTTCAGATGATAAAATCTTTACTGATATTTTTCACTACCTGGAG
  GTGTTGTTTCGCATTATTAAACCCAGGAAAGTGTTCTTTATGGCTGTAGATGGTGTGGCTCCTCGAGCA
  AAAATGAACCAGCAGCGTGGGAGGCGTTTTAGGTCAGCAAAGGAGGCAGAAGACAAAATTAAAAAGGCA
  ATAGAGAAGGGAGAAACTCTTCCTACAGAGGCCAGATTTGATTCCAACTGTATCACACCAGGAACTGAA
  TTTATGGCCAGGTTACATGAACATCTGAAGTATTTTGTAAATATGAAAATTTCCACAGACAAGTCATGG
  CAAGGAGTTACCATCTACTTCTCAGGCCATGAGACTCCTGGAGAAGGAGAGCATAAAATCATGGAATTT
  ATCAGATCCGAGAAAGCAAAGCCAGATCATGATCCAAACACCAGACACTGTCTTTATGGTTTAGATGCT
  GACTTGATTATGCTTGGATTAACAAGTCATGAGGCACATTTTTCTCTCTTAAGAGAAGAAGTTCGATTT
  GGTGGCAAAAAAACACAACGGGTATGTGCTCCAGAAGAAACTACATTTCACCTTCTACACTTGTCTTTA
  ATGAGAGAGTATATTGACTATGAGTTTTCAGTATTAAAAGAAAAGATCACATTTAAATATGATATTGAA
  AGGATAATAGATGATTGGATTTTGATGGGGTTTCTTGTTGGTAATGATTTTATCCCTCATCTACCTCAT
  TTACATATTAATCATGATGCACTGCCTCTTCTTTATGGAACATATGTTACCATCCTGCCAGAACTTGGG
  GGTTATATTAATGAAAGTGGGCACCTCAACTTACCTCGATTTGAGAAATACCTTGTGAAACTATCAGAT
  TTTGATCGGGAGCACTTCAGTGAAGTTTTTGTGGACCTAAAATGGTTTGAAAGCAAAGTTGGTAACAAG
  TACCTCAATGAAGCAGCAGGTGTCGCAGCAGAAGAAGCCAGGAACTACAAGGAAAAGAAAAAGTTAAAG
  GGCCAGGAAAATTCTCTGTGTTGGACTGCTTTAGACAAAAATGAAGGCGAAATGATAACTTCTAAGGAT
  AATTTAGAAGATGAGACTGAAGATGATGACCTATTTGAAACTGAGTTTAGACAATATAAAAGAACATAT
  TACATGACGAAGATGGGGGTTGACGTAGTATCTGATGACTTTCTGGCTGATCAAGCTGCATGTTATGTT
  CAGGCAATACAGTGGATTTTGCACTATTACTATCATGGAGTTCAGTCCTGGAGCTGGTATTATCCTTAT
  CATTATGCACCTTTCCTGTCTGATATACACAACATCAGTACACTCAAAATCCATTTTGAACTAGGAAAA
  CCTTTTAAGCCATTTGAACAGCTTCTTGCTGTACTTCCAGCAGCCAGCAAAAATTTACTTCCTGCATGC
  TACCAGCATTTGATGACCAATGAAGACTCACCAATTATAGAATATTACCCACCTGATTTTAAAACTGAC
  CTAAATGGGAAACAACAGGAATGGGAAGCTGTGGTGTTAATCCCTTTTATTGATGAGAAGCGATTATTG
  GAAGCCATGGAGACATGTAACCACTCCCTCAAAAAGGAAGAGAGGAAAAGAAACCAACATAGTGAGTGC
  CTAATGTGCTGGTATGATAGAGACACAGAGTTTATCTATCCTTCTCCATGGCCAGAAAAGTTCCCTGCC
  ATAGAACGATGTTGTACAAGGTATAAAATAATATCCTTAGATGCTTGGCGTGTAGACATAAACAAAAAC
  AAAATAACCAGAATTGACCAGAAAGCATTATATTTCTGTGGATTTCCTACTCTGAAACACATCAGACAC
  AAATTTTTTTTGAAGAAAAGTGGTGTTCAAGTATTCCAGCAAAGCAGTCGTGGAGAAAACATGATGTTG
  GAAATCTTAGTGGATGCAGAATCAGATGAACTTACCGTAGAAAATGTAGCTTCATCAGTGCTTGGAAAA
  TCTGTCTTTGTTAATTGGCCTCACCTTGAGGAAGCTAGAGTCGTGGCTGTATCAGATGGAGAAACTAAG
  TTTTACTTGGAAGAACCTCCAGGAACACAGAAGCTTTATTCAGGAAGAACTGCCCCACCATCTAAAGTG
  GTTCATCTTGGAGATAAAGAACAATCTAACTGGGCAAAAGAAGTACAAGGAATTTCAGAACACTACCTG
  AGAAGAAAAGGAATAATAATAAATGAAACATCTGCAGTTGTGTATGCTCAGTTACTCACAGGTCGTAAA
  TATCAAATAAATCAAAATGGTGAAGTTCGTCTAGAGAAACAGTGGTCAAAACAAGTTGTTCCTTTTGTT
  TATCAAACTATTGTCAAGGACATCCGAGCTTTCGACTCCCGTTTCTCCAATATCAAAACATTGGATGAT
  TTGTTTCCTCTGAGAAGTATGGTCTTTATGCTGGGAACTCCCTATTATGGCTGCACTGGAGAAGTTCAG
  GATTCAGGTGATGTGATTACAGAAGGTAGGATTCGTGTGATTTTCAGCATTCCATGTGAACCCAATCTT
  GATGCTTTAATACAGAACCAGCATAAATATTCTATAAAGTACAACCCAGGATATGTGTTGGCCAGTCGC
  CTTGGAGTGAGTGGATACCTTGTTTCAAGGTTTACAGGAAGTATTTTTATTGGAAGAGGATCTAGGAGA
  AACCCTCATGGAGACCATAAAGCAAATGTGGGTTTAAATCTCAAATTCAACAAGAAAAATGAGGAGGTA
  CCTGGATATACTAAGAAAGTTGGAAGTGAATGGATGTATTCATCTGCAGCAGAACAACTTCTGGCAGAG
  TACTTAGAGAGAGCTCCAGAACTATTTAGTTATATAGCCAAAAATAGCCAAGAGGATGTGTTCTATGAA
  GATGACATTTGGCCTGGAGAAAATGAGAATGGTGCTGAAAAAGTTCAAGAAATTATTACTTGGCTAAAA
  GGACATCCTGTCAGTACTTTATCTCGTTCTTCTTGTGATTTACAAATTCTGGATGCAGCTATTGTTGAG
  AAAATTGAGGAAGAAGTCGAAAAGTGCAAGCAAAGAAAGAATAATAAGAAGGTGCGAGTAACAGTGAAA
  CCCCATTTGCTATACAGACCTTTAGAACAGCAACATGGAGTCATTCCTGATCGGGATGCAGAATTTTGT
  CTTTTTGACCGTGTTGTAAATGTGAGAGAAAACTTCTCAGTTCCAGTTGGCCTTCGAGGCACCATCATA
  GGAATAAAAGGAGCTAATAGAGAAGCCGATGTACTATTTGAAGTATTATTTGATGAAGAATTTCCTGGA
  GGGTTAACAATAAGATGCTCACCTGGTAGAGGTTATCGACTGCCAACAAGTGCCTTGGTGAACCTTTCT
  CATGGGAGTCGCTCTGAAACTGGAAATCAGAAGTTGACAGCCATCGTAAAACCACAACCAGCTGTACAT
  CAACATAGCTCAAGTTCATCAGTTTCCTCTGGGCATTTGGGAGCCCTCAACCATTCCCCTCAATCACTT
  TTTGTTCCTACTCAAGTACCTACTAAAGATGATGATGAATTCTGCAACATTTGGCAGTCCTTACAGGGA
  TCTGGAAAGATGCAATACTTTCAGCCAACTATACAAGAGAAGGGTGCAGTTCTACCTCAAGAAATAAGC
  CAAGTAAATCAACATCATAAATCTGGCTTTAATGACAACAGTGTTAAATATCAGCAAAGAAAACATGAC
  CCTCACAGAAAATTTAAAGAAGAGTGTAAGAGTCCTAAAGCTGAGTGTTGGTCCCAAAAAATGTCCAAT
  AAGCAGCCTAACTCTGGAATTGAGAACTTTTTAGCATCTTTGAATATCTCCAAAGAAAATGAAGTACAG
  TCATCTCATCATGGGGAGCCTCCAAGTGAAGAGCATTTGTCACCACAGTCATTTGCCATGAAGGGAACA
  CGGATGCTTAAAGAAATTCTAAAAATTGATGGCTCTAACACTGTGGACCATAAGAATGAAATCAAACAG
  ATTGCTAATGAAATCCCTGTTTCCTCTAACAGAAGAGATGAATATGGATTACCCTCTCAGCCTAAACAA
  AATAAGAAATTAGCATCTTATATGAACAAGCCTCACAGTGCTAATGAGTACCATAATGTTCAGTCTATG
  GACAATATGTGTTGGCCTGCCCCCAGCCAGATCCCTCCTGTATCCACACCAGTAACTGAACTTTCTCGA
  ATTTGTTCCCTTGTTGGAATGCCACAACCTGATTTCTCCTTTCTTAGGATGCCACAGACAATGACCGTT
  TGCCAAGTAAAATTATCTAATGGCTTACTGGTACATGGGCCACAGTGCCACTCTGAAAATGAAGCCAAA
  GAGAAAGCTGCACTTTTTGCTTTACAACAGTTGGGCTCCTTAGGCATGAATTTCCCTTTGCCTTCACAA
  GTATTTGCAAATTATCCTTCAGCTGTACCACCTGGAACCATTCCTCCAGCCTTTCCCCCACCTACTGCT
  AATATAATGCCTTCGTCGTCTCATCTCTTTGGCTCAATGCCATGGGGACCATCGGTGCCAGTTCCTGGG
  AAGCCCTTCCATCATACTTTATATTCTGGGACCATGCCCATGGCTGGGGGAATACCAGGGGGTGTGCAC
  AATCAGTTTATACCTCTGCAGGTTACTAAAAAAAGGGTTGCAAACAAAAAGAACTTTGAGAATAAGGAA
  GCCCAGAGTTCTCAAGCCACTCCAGTTCAGACTAGCCAGCCAGATTCTTCCAACATTGTCAAAGTAAGT
  CCACGGGAGAGCTCATCAGCTTCTTTGAAGTCCTCTCCGATTGCTCAACCTGCATCTTCTTTTCAAGTT
  GAAACTGCCTCTCAAGGCCATAGTATATCTCACCATAAGTCAACACCAATCTCTTCTTCAAGAAGAAAA
  TCAAGAAAACTGGCTGTTAATTTTGGTGTTTCTAAACCTTCTGAG
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG240201
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MGVPKFYRWISERYPCLSEVVKEHQIPEFDNLYLDMNGIIHQCSHPNDDDVHFRISDDKIFTDIFHYLE
  VLFRIIKPRKVFFMAVDGVAPRAKMNQQRGRRFRSAKEAEDKIKKAIEKGETLPTEARFDSNCITPGTE
  FMARLHEHLKYFVNMKISTDKSWQGVTIYFSGHETPGEGEHKIMEFIRSEKAKPDHDPNTRHCLYGLDA
  DLIMLGLTSHEAHFSLLREEVRFGGKKTQRVCAPEETTFHLLHLSLMREYIDYEFSVLKEKITFKYDIE
  RIIDDWILMGFLVGNDFIPHLPHLHINHDALPLLYGTYVTILPELGGYINESGHLNLPRFEKYLVKLSD
  FDREHFSEVFVDLKWFESKVGNKYLNEAAGVAAEEARNYKEKKKLKGQENSLCWTALDKNEGEMITSKD
  NLEDETEDDDLFETEFRQYKRTYYMTKMGVDVVSDDFLADQAACYVQAIQWILHYYYHGVQSWSWYYPY
  HYAPFLSDIHNISTLKIHFELGKPFKPFEQLLAVLPAASKNLLPACYQHLMTNEDSPIIEYYPPDFKTD
  LNGKQQEWEAVVLIPFIDEKRLLEAMETCNHSLKKEERKRNQHSECLMCWYDRDTEFIYPSPWPEKFPA
  IERCCTRYKIISLDAWRVDINKNKITRIDQKALYFCGFPTLKHIRHKFFLKKSGVQVFQQSSRGENMML
  EILVDAESDELTVENVASSVLGKSVFVNWPHLEEARVVAVSDGETKFYLEEPPGTQKLYSGRTAPPSKV
  VHLGDKEQSNWAKEVQGISEHYLRRKGIIINETSAVVYAQLLTGRKYQINQNGEVRLEKQWSKQVVPFV
  YQTIVKDIRAFDSRFSNIKTLDDLFPLRSMVFMLGTPYYGCTGEVQDSGDVITEGRIRVIFSIPCEPNL
  DALIQNQHKYSIKYNPGYVLASRLGVSGYLVSRFTGSIFIGRGSRRNPHGDHKANVGLNLKFNKKNEEV
  PGYTKKVGSEWMYSSAAEQLLAEYLERAPELFSYIAKNSQEDVFYEDDIWPGENENGAEKVQEIITWLK
  GHPVSTLSRSSCDLQILDAAIVEKIEEEVEKCKQRKNNKKVRVTVKPHLLYRPLEQQHGVIPDRDAEFC
  LFDRVVNVRENFSVPVGLRGTIIGIKGANREADVLFEVLFDEEFPGGLTIRCSPGRGYRLPTSALVNLS
  HGSRSETGNQKLTAIVKPQPAVHQHSSSSSVSSGHLGALNHSPQSLFVPTQVPTKDDDEFCNIWQSLQG
  SGKMQYFQPTIQEKGAVLPQEISQVNQHHKSGFNDNSVKYQQRKHDPHRKFKEECKSPKAECWSQKMSN
  KQPNSGIENFLASLNISKENEVQSSHHGEPPSEEHLSPQSFAMKGTRMLKEILKIDGSNTVDHKNEIKQ
  IANEIPVSSNRRDEYGLPSQPKQNKKLASYMNKPHSANEYHNVQSMDNMCWPAPSQIPPVSTPVTELSR
  ICSLVGMPQPDFSFLRMPQTMTVCQVKLSNGLLVHGPQCHSENEAKEKAALFALQQLGSLGMNFPLPSQ
  VFANYPSAVPPGTIPPAFPPPTANIMPSSSHLFGSMPWGPSVPVPGKPFHHTLYSGTMPMAGGIPGGVH
  NQFIPLQVTKKRVANKKNFENKEAQSSQATPVQTSQPDSSNIVKVSPRESSSASLKSSPIAQPASSFQV
  ETASQGHSISHHKSTPISSSRRKSRKLAVNFGVSKPSE
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001282857
• ORF Size: 5082 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).

Reference Data

• RefSeq: NM_001282857.2
• RefSeq Size: 10107 bp
• RefSeq ORF: 5085 bp
• Locus ID: 54464
• UniProt ID: Q8IZH2
• Cytogenetics: 3q23
• Protein Pathways: RNA degradation
• MW: 193.3 kDa
• Gene Summary: This gene encodes a member of the 5'-3' exonuclease family. The encoded protein may be involved in replication-dependent histone mRNA degradation, and interacts directly with the enhancer of mRNA-decapping protein 4. In addition to mRNA metabolism, a similar protein in yeast has been implicated in a variety of nuclear and cytoplasmic functions, including homologous recombination, meiosis, telomere maintenance, and microtubule assembly. Mutations in this gene are associated with osteosarcoma, suggesting that the encoded protein may also play a role in bone formation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]