GAPex 5 (GAPVD1) (NM_001282680) Human Tagged ORF Clone

CAT#: RG240155

GAPVD1 (tGFP-tagged) - Human GTPase activating protein and VPS9 domains 1 (GAPVD1), transcript variant 2

Product Images

Circular map for RG240155

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: GAPex 5
• Synonyms: GAPex-5; GAPEX5; RAP6
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG240155 representing NM_001282680.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGTGAAACTAGATATTCATACTCTGGCTCATCACCTCAAGCAGGAACGCTTATATGTAAACTCTGAG
  AAACAGCTCATTCAGAGGCTCAATGCAGATGTACTTAAGACAGCTGAAAAGTTGTATCGTACAGCATGG
  ATTGCGAAGCAACAGAGAATCAATTTGGATCGGCTTATCATAACCAGTGCTGAAGCTTCCCCTGCTGAA
  TGTTGCCAACATGCCAAAATTTTGGAAGATACACAATTTGTTGATGGGTATAAGCAATTGGGATTTCAG
  GAGACTGCTTATGGAGAATTCTTGAGTCGATTGAGGGAAAATCCTCGTCTTATTGCCTCCTCTTTGGTT
  GCTGGAGAGAAACTTAATCAGGAGAACACACAAAGTGTTATTTACACAGTTTTTACCTCCCTGTATGGC
  AATTGCATCATGCAAGAAGATGAAAGCTACCTCCTTCAGGTTTTGCGATACTTGATTGAATTTGAACTT
  AAAGAAAGTGACAACCCTAGGCGACTTTTGAGGAGAGGAACTTGTGCCTTCAGCATCTTATTTAAACTT
  TTTTCTGAAGGACTGTTTTCTGCCAAACTTTTCCTCACAGCCACTTTACATGAGCCAATTATGCAACTG
  CTTGTTGAAGATGAAGATCACCTGGAAACAGATCCAAACAAGCTAATTGAGAGGTTCTCTCCATCTCAG
  CAGGAAAAACTCTTTGGAGAGAAGGGCTCAGATAGATTCAGGCAAAAAGTTCAAGAAATGGTGGAGTCC
  AATGAAGCAAAGCTAGTGGCTTTGGTGAACAAATTTATTGGTTATCTCAAACAGAACACATATTGTTTT
  CCACATAGTTTAAGGTGGATCGTGTCTCAGATGTACAAAACCCTCTCCTGTGTAGATAGGCTGGAAGTT
  GGGGAGGTCAGGGCAATGTGTACTGATCTCCTGTTGGCCTGCTTCATTTGTCCTGCAGTTGTCAATCCA
  GAACAATATGGAATAATTTCCGATGCTCCTATTAATGAAGTAGCACGATTTAATCTGATGCAGGTAGGC
  CGCCTTTTGCAGCAGTTAGCAATGACTGGCTCTGAAGAGGGAGATCCCCGAACAAAGAGCAGCCTTGGA
  AAGTTTGACAAAAGCTGTGTTGCCGCTTTCCTTGATGTTGTGATTGGGGGCCGTGCAGTGGAGACCCCT
  CCATTGTCTTCCGTCAATCTTCTGGAAGGATTGAGCAGAACTGTGGTTTATATAACCTACAGTCAGCTT
  ATTACTCTGGTGAATTTTATGAAGAGTGTGATGTCTGGAGATCAACTGAGAGAAGATAGAATGGCTCTT
  GACAATTTATTGGCAAACCTACCCCCGGCCAAGCCAGGAAAAAGTAGCAGTTTAGAAATGACTCCCTAC
  AATACACCTCAGCTATCTCCAGCAACCACTCCAGCAAATAAAAAGAATCGATTACCTATAGCAACTCGG
  AGCAGAAGCCGCACCAATATGCTAATGGACCTACATATGGACCATGAAGGATCATCTCAAGAAACCATC
  CAGGAGGTGCAACCAGAAGAGGTGTTGGTCATTTCCTTAGGTACAGGTCCCCAGCTTACTCCAGGGATG
  ATGTCAGAAAATGAGGTCCTAAACATGCAGCTTTCGGATGGAGGACAAGGAGATGTCCCTGTTGATGAA
  AACAAACTCCATGGTAAACCTGATAAAACCTTGCGCTTTTCCCTCTGCAGTGATAATCTGGAAGGAATA
  TCTGAAGGTCCTTCAAATCGCTCCAATTCAGTGTCCTCCCTAGACCTAGAAGGAGAGTCTGTGTCAGAA
  CTTGGAGCAGGACCTTCTGGCAGTAATGGAGTTGAAGCTCTACAGCTGTTAGAACATGAGCAAGCTACA
  ACACAGGATAACCTTGATGATAAGCTAAGGAAGTTTGAAATTCGTGACATGATGGGATTAACAGATGAT
  AGGGACATATCAGAAACAGTGAGTGAGACCTGGAGTACAGACGTCTTGGGAAGTGACTTTGACCCTAAT
  ATTGATGAAGATCGCTTGCAAGAAATTGCAGGTGCTGCAGCAGAGAACATGTTAGGCAGTTTGCTGTGC
  CTCCCAGGTTCAGGGTCAGTGCTTCTTGACCCCTGCACTGGTTCTACCATATCAGAGACAACAAGTGAA
  GCTTGGAGTGTAGAGGTATTGCCAAGTGACTCAGAGGCCCCAGACCTAAAGCAGGAGGAGCGTCTGCAA
  GAACTGGAGAGCTGTTCTGGACTGGGTAGCACATCTGATGATACGGATGTCAGGGAGGTCAGTTCCCGC
  CCCAGCACACCAGGCCTCAGTGTTGTGTCCGGCATAAGTGCAACCTCTGAGGATATTCCCAATAAGATT
  GAAGACCTGAGATCTGAGTGCAGCTCTGATTTTGGGGGTAAAGATTCTGTCACTAGTCCAGACATGGAT
  GAAATAACTCACGGTGCCCACCAGCTGACCTCTCCTCCTTCTCAGTCAGAGTCTCTGCTGGCCATGTTT
  GATCCACTGTCTTCACATGAAGGGGCTTCTGCTGTGGTAAGGCCAAAGGTTCACTATGCTAGGCCATCG
  CATCCACCACCAGATCCCCCAATCCTGGAAGGAGCTGTGGGAGGAAATGAGGCCAGGTTGCCAAACTTT
  GGTTCCCATGTTTTAACTCCAGCTGAAATGGAGGCATTCAAGCAAAGGCATTCTTACCCTGAGAGACTA
  GTTCGAAGCAGGAGCTCTGATATAGTATCTTCTGTCCGGAGACCCATGAGTGACCCCAGCTGGAACCGG
  CGTCCAGGAAATGAAGAGCGAGAACTCCCTCCAGCTGCAGCCATTGGTGCTACTTCTTTGGTGGCTGCA
  CCTCATTCATCATCTTCATCCCCGAGTAAGGACTCCTCAAGAGGAGAGACTGAAGAACGCAAAGATAGC
  GATGATGAGAAATCAGACAGGAACAGACCTTGGTGGAGAAAACGTTTTGTTTCAGCCATGCCTAAAGCT
  CCTATACCATTTAGAAAGAAAGAAAAACAAGAAAAAGACAAAGATGATCTGGGGCCTGACAGATTCTCA
  ACACTCACAGATGATCCCAGCCCTAGACTCAGTGCACAAGCTCAGGTGGCTGAGGATATTCTGGACAAA
  TACAGGAATGCCATTAAACGGACCAGCCCCAGTGATGGAGCAATGGCAAACTATGAAAGTACAGAGGTT
  ATGGGTGATGGTGAAAGTGCACATGATTCTCCCCGTGACGAAGCACTGCAGAACATCTCGGCTGATGAT
  CTCCCAGACTCTGCAAGCCAAGCAGCCCACCCGCAGGATTCAGCTTTCTCTTACAGAGATGCAAAAAAG
  AAACTGAGGCTTGCTCTTTGCTCTGCGGACTCTGTTGCCTTCCCAGTGCTGACCCATTCAACAAGGAAT
  GGTTTACCAGACCACACAGACCCAGAAGACAATGAAATTGTATGCTTCTTAAAAGTTCAAATAGCTGAA
  GCAATTAATTTACAAGATAAGAATCTAATGGCTCAACTTCAAGAAACAATGCGCTGTGTGTGCCGTTTT
  GATAATAGGACTTGTAGGAAACTGCTGGCTTCGATTGCTGAGGACTACAGAAAAAGAGCCCCATATATT
  GCTTATCTCACTCGTTGTCGACAAGGACTACAGACCACACAGGCTCACCTGGAAAGGCTATTGCAAAGA
  GTTTTGCGGGACAAAGAAGTGGCCAATCGATACTTTACCACTGTCTGTGTGAGATTACTGCTTGAGAGC
  AAAGAAAAGAAGATCAGGGAATTCATTCAAGACTTTCAGAAACTCACCGCAGCTGACGATAAAACTGCT
  CAGGTAGAAGATTTTCTGCAGTTTCTTTATGGTGCAATGGCCCAGGATGTCATATGGCAAAACGCGAGT
  GAAGAACAGCTTCAAGATGCACAGCTGGCCATTGAGCGAAGCGTGATGAACCGGATTTTCAAGCTCGCC
  TTCTACCCTAATCAAGATGGGGACATACTTCGCGACCAGGTTCTTCATGAACATATCCAGAGATTGTCT
  AAAGTAGTGACTGCAAATCACAGAGCTCTTCAGATACCAGAGGTTTATCTTCGAGAAGCACCATGGCCA
  TCTGCACAATCAGAAATCAGGACAATAAGTGCTTATAAAACCCCCCGGGACAAAGTGCAGTGCATCCTG
  AGAATGTGCTCTACGATTATGAACCTCCTGAGCCTGGCCAATGAGGACTCTGTCCCTGGAGCGGATGAC
  TTTGTTCCTGTGTTGGTGTTTGTGTTGATAAAGGCAAATCCACCCTGTTTGCTGTCTACTGTGCAGTAT
  ATCAGTAGCTTTTATGCTAGCTGTCTGTCTGGAGAGGAGTCCTATTGGTGGATGCAGTTCACAGCAGCA
  GTAGAATTCATTAAAACCATCGATGACCGAAAG
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG240155
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MVKLDIHTLAHHLKQERLYVNSEKQLIQRLNADVLKTAEKLYRTAWIAKQQRINLDRLIITSAEASPAE
  CCQHAKILEDTQFVDGYKQLGFQETAYGEFLSRLRENPRLIASSLVAGEKLNQENTQSVIYTVFTSLYG
  NCIMQEDESYLLQVLRYLIEFELKESDNPRRLLRRGTCAFSILFKLFSEGLFSAKLFLTATLHEPIMQL
  LVEDEDHLETDPNKLIERFSPSQQEKLFGEKGSDRFRQKVQEMVESNEAKLVALVNKFIGYLKQNTYCF
  PHSLRWIVSQMYKTLSCVDRLEVGEVRAMCTDLLLACFICPAVVNPEQYGIISDAPINEVARFNLMQVG
  RLLQQLAMTGSEEGDPRTKSSLGKFDKSCVAAFLDVVIGGRAVETPPLSSVNLLEGLSRTVVYITYSQL
  ITLVNFMKSVMSGDQLREDRMALDNLLANLPPAKPGKSSSLEMTPYNTPQLSPATTPANKKNRLPIATR
  SRSRTNMLMDLHMDHEGSSQETIQEVQPEEVLVISLGTGPQLTPGMMSENEVLNMQLSDGGQGDVPVDE
  NKLHGKPDKTLRFSLCSDNLEGISEGPSNRSNSVSSLDLEGESVSELGAGPSGSNGVEALQLLEHEQAT
  TQDNLDDKLRKFEIRDMMGLTDDRDISETVSETWSTDVLGSDFDPNIDEDRLQEIAGAAAENMLGSLLC
  LPGSGSVLLDPCTGSTISETTSEAWSVEVLPSDSEAPDLKQEERLQELESCSGLGSTSDDTDVREVSSR
  PSTPGLSVVSGISATSEDIPNKIEDLRSECSSDFGGKDSVTSPDMDEITHGAHQLTSPPSQSESLLAMF
  DPLSSHEGASAVVRPKVHYARPSHPPPDPPILEGAVGGNEARLPNFGSHVLTPAEMEAFKQRHSYPERL
  VRSRSSDIVSSVRRPMSDPSWNRRPGNEERELPPAAAIGATSLVAAPHSSSSSPSKDSSRGETEERKDS
  DDEKSDRNRPWWRKRFVSAMPKAPIPFRKKEKQEKDKDDLGPDRFSTLTDDPSPRLSAQAQVAEDILDK
  YRNAIKRTSPSDGAMANYESTEVMGDGESAHDSPRDEALQNISADDLPDSASQAAHPQDSAFSYRDAKK
  KLRLALCSADSVAFPVLTHSTRNGLPDHTDPEDNEIVCFLKVQIAEAINLQDKNLMAQLQETMRCVCRF
  DNRTCRKLLASIAEDYRKRAPYIAYLTRCRQGLQTTQAHLERLLQRVLRDKEVANRYFTTVCVRLLLES
  KEKKIREFIQDFQKLTAADDKTAQVEDFLQFLYGAMAQDVIWQNASEEQLQDAQLAIERSVMNRIFKLA
  FYPNQDGDILRDQVLHEHIQRLSKVVTANHRALQIPEVYLREAPWPSAQSEIRTISAYKTPRDKVQCIL
  RMCSTIMNLLSLANEDSVPGADDFVPVLVFVLIKANPPCLLSTVQYISSFYASCLSGEESYWWMQFTAA
  VEFIKTIDDRK
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001282680
• ORF Size: 4380 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).

Reference Data

• RefSeq: NM_001282680.2
• RefSeq Size: 7029 bp
• RefSeq ORF: 4383 bp
• Locus ID: 26130
• UniProt ID: Q14C86
• Cytogenetics: 9q33.3
• MW: 163.3 kDa
• Gene Summary: Acts both as a GTPase-activating protein (GAP) and a guanine nucleotide exchange factor (GEF), and participates in various processes such as endocytosis, insulin receptor internalization or LC2A4/GLUT4 trafficking. Acts as a GEF for the Ras-related protein RAB31 by exchanging bound GDP for free GTP, leading to regulate LC2A4/GLUT4 trafficking. In the absence of insulin, it maintains RAB31 in an active state and promotes a futile cycle between LC2A4/GLUT4 storage vesicles and early endosomes, retaining LC2A4/GLUT4 inside the cells. Upon insulin stimulation, it is translocated to the plasma membrane, releasing LC2A4/GLUT4 from intracellular storage vesicles. Also involved in EGFR trafficking and degradation, possibly by promoting EGFR ubiquitination and subsequent degradation by the proteasome. Has GEF activity for Rab5 and GAP activity for Ras.[UniProtKB/Swiss-Prot Function]