KIAA0528 (C2CD5) (NM_001286177) Human Tagged ORF Clone

CAT#: RG239909

C2CD5 (tGFP-tagged) - Human C2 calcium-dependent domain containing 5 (C2CD5), transcript variant 5

Product Images

Circular map for RG239909

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: KIAA0528
• Synonyms: CDP138; KIAA0528
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239909 representing NM_001286177.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCCAGGGAAGCTGAAGGTGAAAATCGTGGCCGGGCGCCATTTGCCAGTGATGGACCGTGCTAGTGAC
  CTGACTGATGCCTTCGTGGAGGTAAAATTTGGTAATACCACCTTTAAAACAGATGTGTACCTTAAGTCA
  CTCAACCCTCAGTGGAACTCGGAGTGGTTTAAATTTGAGGTGGATGATGAAGACTTACAAGATGAACCT
  TTACAGATCACAGTTCTTGACCATGATACTTACAGTGCAAATGATGCCATTGGTAAAGTGTACATTGAT
  ATTGATCCTTTACTGTATAGTGAAGCTGCAACAGTCATCTCAGGATGGTTTCCAATTTATGACACCATA
  CATGGTATCCGTGGGGAAATCAATGTAGTTGTCAAAGTAGACCTCTTCAATGATTTAAATCGATTTAGG
  CAGTCATCATGTGGAGTCAAATTCTTTTGCACAACGTCTATTCCAAAATGCTATAGAGCTGTGATAATT
  CATGGATTTGTAGAAGAACTTGTGGTCAATGAAGACCCAGAATATCAGTGGATTGATCGAATTCGCACA
  CCAAGGGCATCAAATGAGGCCAGACAGAGACTCATTTCGTTAATGTCAGGTGAGCTGCAGAGGAAGATT
  GGCTTGAAAGTACTTGAAATGAGAGGAAATGCAGTTGTGGGGTACTTACAGTGTTTCGATCTGGAGGGC
  GAGTCTGGGTTAGTGGTGCGAGCCATAGGAACGGCGTGTACTCTGGATAAATTAAGTAGCCCAGCAGCA
  TTCCTTCCTGCATGTAATTCCCCATCCAAAGAAATGAAGGAGTCTCCGCTGGTTCATCCTCCAAGCCAT
  GGATGCCGCTCGACTCACAACAGCCCAATTCACACTGCTACTGGCTCACGACTTACTCAGAACTTCTCT
  GTGTCTGTTCCCACTCTCATCTATACTGGAATGGGAAGTGGTAGTGCTGGAAAAGAAGGGGGGCCCTTT
  AAAGCTCTTTTAAGACAACAGACTCAATCAGCGTTGGAACAGAGGGAATTTCCATTTTTTACCTTGACG
  GCATTTCCTCCTGGATTCCTTGTACACGTTGGGGGTGTAGTTAGTGCACGTTCTGTGAAGCTTTTGGAT
  CGTATCCACAATCCTGATGAACCAGAAACTCGAGATGCATGGTGGGCAGAAATCAGACAAGAAATAAAA
  TCACATGCTAAAGCATTAGGCTGTCATGCTGTAGTGGGCTACAGTGAATCAACTAGCATCTGTGAAGAG
  GTCTGCATTTTATCTGCATCTGGCACAGCGGCTGTACTGAATCCTAGATTTCTGCAGGATGGCACCGTG
  GAAGGCTGTTTGGAACAGAGGCTTGAAGAAAATTTGCCTACACGTTGTGGATTTTGTCATATACCATAT
  GATGAACTGAATATGCCATTTCCAGCTCATCTCACATATTGCTATAACTGCAGGAAACAAAAAGTTCCT
  GATGTTCTGTTTACAACTATAGACCTCCCAACAGATGCAACAGTTATTGGAAAAGGTTGTCTTATTCAA
  GCAAGGTTATGTCGCTTAAAAAAGAAAGCACAGGCAGAAGCAAATGCTACAGCTATCAGTAATCTCTTG
  CCATTTATGGAATATGAAGTGCATACTCAGCTAATGAATAAACTAAAACTCAAAGGAATGAATGCTTTG
  TTTGGACTAAGAATTCAGATCACAGTGGGTGAAAATATGTTGATGGGCTTAGCGTCTGCCACAGGTGTG
  TATTTAGCAGCTTTACCAACTCCTGGTGGTATTCAGATTGCTGGGAAGACTCCTAATGATGGCTCATAT
  GAACAACACATCTCTCATATGCAGAAGAAGATAAATGACACAATTGCTAAAAACAAAGAGTTATATGAA
  ATCAATCCTCCAGAGATATCTGAAGAGATTATAGGATCACCCATCCCAGAACCTAGGCAACGCTCAAGA
  CTTCTAAGATCTCAATCAGAAAGCTCGGATGAAGTTACAGAATTAGACCTTTCACATGGGAAAAAAGAT
  GCTTTTGTTTTGGAGATTGATGACACAGATGCCATGGAAGATGTCCATTCTCTACTTACTGATGTTCCT
  CCTCCTTCAGGCTTTTATAGTTGTAATACAGAAATTATGCCCGGTATAAATAATTGGACCTCTGAAATA
  CAGATGTTCACTTCAGTAAGAGTAATCAGATTAAGCAGCCTCAACCTGACTAATCAAGCTCTCAATAAG
  AACTTTAATGATCTCTGTGAAAATTTGCTCAAGAGCCTGTACTTTAAACTACGATCTATGATCCCCTGC
  TGCCTTTGCCATGTAAATTTTACAGTATCTCTGCCTGAAGATGAATTAATTCAGGTTACAGTCACGGCA
  GTCGCAATCACTTTTGACAAAAATCAGGCTTTACAAACCACAAAAACCCCTGTTGAAAAGTCATTGCAA
  AGAGCCTCAACAGATAATGAAGAACTTTTACAATTTCCACTGGAACTGTGTTCAGATTCACTTCCTTCT
  CATCCTTTTCCACCAGCTAAAGAACACCTGGAGAGTGCAAGTTCTAACTCAGGTATACCAGCTGCACAG
  AGAGCAACGTCAGTTGATTACAGTTCCTTTGCAGACAGATGCAGCAGCTGGATAGAGCTCATTAAACTG
  AAAGCTCAGACCATAAGGCGTGGATCAATTAAGACAACCATGACAGTTGAAAAAGCAAGTCCAGTGGGT
  GATGGAAATTTCCGGAATCGTTCTGCTCCACCTTGTGCAAATTCCACAGTTGGGGTTGTTAAGATGACA
  CCTCTTTCTTTTATTCCTGGAGCAAAAATAACTAAGTATCTTGGGATAATTAACATGTTTTTTATTCGA
  GAGACTACTTCTCTTCGGGAGGAAGGAGGAGTCAGTGGTTTTCTCCATGCTTTTATTGCTGAAGTGTTT
  GCAATGGTGAGAGCTCATGTTGCTGCATTAGGAGGGAATGCTGTTGTCTCCTACATAATGAAGCAGTGT
  GTCTTCATGGAGAATCCAAATAAAAACCAGGCACAGTGTCTTATAAATGTAAGTGGTGATGCAGTGGTT
  TTTGTTCGTGAATCTGACTTAGAAGTGGTGTCATCTCAGCAACCTACTACCAACTGCCAGTCATCATGT
  ACTGAAGGCGAAGTTACAACC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239909
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MPGKLKVKIVAGRHLPVMDRASDLTDAFVEVKFGNTTFKTDVYLKSLNPQWNSEWFKFEVDDEDLQDEP
  LQITVLDHDTYSANDAIGKVYIDIDPLLYSEAATVISGWFPIYDTIHGIRGEINVVVKVDLFNDLNRFR
  QSSCGVKFFCTTSIPKCYRAVIIHGFVEELVVNEDPEYQWIDRIRTPRASNEARQRLISLMSGELQRKI
  GLKVLEMRGNAVVGYLQCFDLEGESGLVVRAIGTACTLDKLSSPAAFLPACNSPSKEMKESPLVHPPSH
  GCRSTHNSPIHTATGSRLTQNFSVSVPTLIYTGMGSGSAGKEGGPFKALLRQQTQSALEQREFPFFTLT
  AFPPGFLVHVGGVVSARSVKLLDRIHNPDEPETRDAWWAEIRQEIKSHAKALGCHAVVGYSESTSICEE
  VCILSASGTAAVLNPRFLQDGTVEGCLEQRLEENLPTRCGFCHIPYDELNMPFPAHLTYCYNCRKQKVP
  DVLFTTIDLPTDATVIGKGCLIQARLCRLKKKAQAEANATAISNLLPFMEYEVHTQLMNKLKLKGMNAL
  FGLRIQITVGENMLMGLASATGVYLAALPTPGGIQIAGKTPNDGSYEQHISHMQKKINDTIAKNKELYE
  INPPEISEEIIGSPIPEPRQRSRLLRSQSESSDEVTELDLSHGKKDAFVLEIDDTDAMEDVHSLLTDVP
  PPSGFYSCNTEIMPGINNWTSEIQMFTSVRVIRLSSLNLTNQALNKNFNDLCENLLKSLYFKLRSMIPC
  CLCHVNFTVSLPEDELIQVTVTAVAITFDKNQALQTTKTPVEKSLQRASTDNEELLQFPLELCSDSLPS
  HPFPPAKEHLESASSNSGIPAAQRATSVDYSSFADRCSSWIELIKLKAQTIRRGSIKTTMTVEKASPVG
  DGNFRNRSAPPCANSTVGVVKMTPLSFIPGAKITKYLGIINMFFIRETTSLREEGGVSGFLHAFIAEVF
  AMVRAHVAALGGNAVVSYIMKQCVFMENPNKNQAQCLINVSGDAVVFVRESDLEVVSSQQPTTNCQSSC
  TEGEVTT
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001286177
• ORF Size: 3126 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).

Reference Data

• RefSeq: NM_001286177.1, NP_001273106.1
• RefSeq Size: 4599 bp
• RefSeq ORF: 3129 bp
• Locus ID: 9847
• UniProt ID: Q86YS7
• Cytogenetics: 12p12.1
• MW: 115.4 kDa
• Gene Summary: Required for insulin-stimulated glucose transport and glucose transporter SLC2A4/GLUT4 translocation from intracellular glucose storage vesicle (GSV) to the plasma membrane (PM) in adipocytes. Binds phospholipid membranes in a calcium-dependent manner and is necessary for the optimal membrane fusion between SLC2A4/GLUT4 GSV and the PM.[UniProtKB/Swiss-Prot Function]