KIAA0528 (C2CD5) (NM_001286177) Human Tagged ORF Clone
CAT#: RG239909
- TrueORF®
C2CD5 (tGFP-tagged) - Human C2 calcium-dependent domain containing 5 (C2CD5), transcript variant 5
ORF Plasmid: DDK
"NM_001286177" in other vectors (2)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | KIAA0528 |
Synonyms | CDP138; KIAA0528 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG239909 representing NM_001286177.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCCAGGGAAGCTGAAGGTGAAAATCGTGGCCGGGCGCCATTTGCCAGTGATGGACCGTGCTAGTGAC CTGACTGATGCCTTCGTGGAGGTAAAATTTGGTAATACCACCTTTAAAACAGATGTGTACCTTAAGTCA CTCAACCCTCAGTGGAACTCGGAGTGGTTTAAATTTGAGGTGGATGATGAAGACTTACAAGATGAACCT TTACAGATCACAGTTCTTGACCATGATACTTACAGTGCAAATGATGCCATTGGTAAAGTGTACATTGAT ATTGATCCTTTACTGTATAGTGAAGCTGCAACAGTCATCTCAGGATGGTTTCCAATTTATGACACCATA CATGGTATCCGTGGGGAAATCAATGTAGTTGTCAAAGTAGACCTCTTCAATGATTTAAATCGATTTAGG CAGTCATCATGTGGAGTCAAATTCTTTTGCACAACGTCTATTCCAAAATGCTATAGAGCTGTGATAATT CATGGATTTGTAGAAGAACTTGTGGTCAATGAAGACCCAGAATATCAGTGGATTGATCGAATTCGCACA CCAAGGGCATCAAATGAGGCCAGACAGAGACTCATTTCGTTAATGTCAGGTGAGCTGCAGAGGAAGATT GGCTTGAAAGTACTTGAAATGAGAGGAAATGCAGTTGTGGGGTACTTACAGTGTTTCGATCTGGAGGGC GAGTCTGGGTTAGTGGTGCGAGCCATAGGAACGGCGTGTACTCTGGATAAATTAAGTAGCCCAGCAGCA TTCCTTCCTGCATGTAATTCCCCATCCAAAGAAATGAAGGAGTCTCCGCTGGTTCATCCTCCAAGCCAT GGATGCCGCTCGACTCACAACAGCCCAATTCACACTGCTACTGGCTCACGACTTACTCAGAACTTCTCT GTGTCTGTTCCCACTCTCATCTATACTGGAATGGGAAGTGGTAGTGCTGGAAAAGAAGGGGGGCCCTTT AAAGCTCTTTTAAGACAACAGACTCAATCAGCGTTGGAACAGAGGGAATTTCCATTTTTTACCTTGACG GCATTTCCTCCTGGATTCCTTGTACACGTTGGGGGTGTAGTTAGTGCACGTTCTGTGAAGCTTTTGGAT CGTATCCACAATCCTGATGAACCAGAAACTCGAGATGCATGGTGGGCAGAAATCAGACAAGAAATAAAA TCACATGCTAAAGCATTAGGCTGTCATGCTGTAGTGGGCTACAGTGAATCAACTAGCATCTGTGAAGAG GTCTGCATTTTATCTGCATCTGGCACAGCGGCTGTACTGAATCCTAGATTTCTGCAGGATGGCACCGTG GAAGGCTGTTTGGAACAGAGGCTTGAAGAAAATTTGCCTACACGTTGTGGATTTTGTCATATACCATAT GATGAACTGAATATGCCATTTCCAGCTCATCTCACATATTGCTATAACTGCAGGAAACAAAAAGTTCCT GATGTTCTGTTTACAACTATAGACCTCCCAACAGATGCAACAGTTATTGGAAAAGGTTGTCTTATTCAA GCAAGGTTATGTCGCTTAAAAAAGAAAGCACAGGCAGAAGCAAATGCTACAGCTATCAGTAATCTCTTG CCATTTATGGAATATGAAGTGCATACTCAGCTAATGAATAAACTAAAACTCAAAGGAATGAATGCTTTG TTTGGACTAAGAATTCAGATCACAGTGGGTGAAAATATGTTGATGGGCTTAGCGTCTGCCACAGGTGTG TATTTAGCAGCTTTACCAACTCCTGGTGGTATTCAGATTGCTGGGAAGACTCCTAATGATGGCTCATAT GAACAACACATCTCTCATATGCAGAAGAAGATAAATGACACAATTGCTAAAAACAAAGAGTTATATGAA ATCAATCCTCCAGAGATATCTGAAGAGATTATAGGATCACCCATCCCAGAACCTAGGCAACGCTCAAGA CTTCTAAGATCTCAATCAGAAAGCTCGGATGAAGTTACAGAATTAGACCTTTCACATGGGAAAAAAGAT GCTTTTGTTTTGGAGATTGATGACACAGATGCCATGGAAGATGTCCATTCTCTACTTACTGATGTTCCT CCTCCTTCAGGCTTTTATAGTTGTAATACAGAAATTATGCCCGGTATAAATAATTGGACCTCTGAAATA CAGATGTTCACTTCAGTAAGAGTAATCAGATTAAGCAGCCTCAACCTGACTAATCAAGCTCTCAATAAG AACTTTAATGATCTCTGTGAAAATTTGCTCAAGAGCCTGTACTTTAAACTACGATCTATGATCCCCTGC TGCCTTTGCCATGTAAATTTTACAGTATCTCTGCCTGAAGATGAATTAATTCAGGTTACAGTCACGGCA GTCGCAATCACTTTTGACAAAAATCAGGCTTTACAAACCACAAAAACCCCTGTTGAAAAGTCATTGCAA AGAGCCTCAACAGATAATGAAGAACTTTTACAATTTCCACTGGAACTGTGTTCAGATTCACTTCCTTCT CATCCTTTTCCACCAGCTAAAGAACACCTGGAGAGTGCAAGTTCTAACTCAGGTATACCAGCTGCACAG AGAGCAACGTCAGTTGATTACAGTTCCTTTGCAGACAGATGCAGCAGCTGGATAGAGCTCATTAAACTG AAAGCTCAGACCATAAGGCGTGGATCAATTAAGACAACCATGACAGTTGAAAAAGCAAGTCCAGTGGGT GATGGAAATTTCCGGAATCGTTCTGCTCCACCTTGTGCAAATTCCACAGTTGGGGTTGTTAAGATGACA CCTCTTTCTTTTATTCCTGGAGCAAAAATAACTAAGTATCTTGGGATAATTAACATGTTTTTTATTCGA GAGACTACTTCTCTTCGGGAGGAAGGAGGAGTCAGTGGTTTTCTCCATGCTTTTATTGCTGAAGTGTTT GCAATGGTGAGAGCTCATGTTGCTGCATTAGGAGGGAATGCTGTTGTCTCCTACATAATGAAGCAGTGT GTCTTCATGGAGAATCCAAATAAAAACCAGGCACAGTGTCTTATAAATGTAAGTGGTGATGCAGTGGTT TTTGTTCGTGAATCTGACTTAGAAGTGGTGTCATCTCAGCAACCTACTACCAACTGCCAGTCATCATGT ACTGAAGGCGAAGTTACAACC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC >Peptide sequence encoded by RG239909
Blue=ORF Red=Cloning site Green=Tag(s) MPGKLKVKIVAGRHLPVMDRASDLTDAFVEVKFGNTTFKTDVYLKSLNPQWNSEWFKFEVDDEDLQDEP LQITVLDHDTYSANDAIGKVYIDIDPLLYSEAATVISGWFPIYDTIHGIRGEINVVVKVDLFNDLNRFR QSSCGVKFFCTTSIPKCYRAVIIHGFVEELVVNEDPEYQWIDRIRTPRASNEARQRLISLMSGELQRKI GLKVLEMRGNAVVGYLQCFDLEGESGLVVRAIGTACTLDKLSSPAAFLPACNSPSKEMKESPLVHPPSH GCRSTHNSPIHTATGSRLTQNFSVSVPTLIYTGMGSGSAGKEGGPFKALLRQQTQSALEQREFPFFTLT AFPPGFLVHVGGVVSARSVKLLDRIHNPDEPETRDAWWAEIRQEIKSHAKALGCHAVVGYSESTSICEE VCILSASGTAAVLNPRFLQDGTVEGCLEQRLEENLPTRCGFCHIPYDELNMPFPAHLTYCYNCRKQKVP DVLFTTIDLPTDATVIGKGCLIQARLCRLKKKAQAEANATAISNLLPFMEYEVHTQLMNKLKLKGMNAL FGLRIQITVGENMLMGLASATGVYLAALPTPGGIQIAGKTPNDGSYEQHISHMQKKINDTIAKNKELYE INPPEISEEIIGSPIPEPRQRSRLLRSQSESSDEVTELDLSHGKKDAFVLEIDDTDAMEDVHSLLTDVP PPSGFYSCNTEIMPGINNWTSEIQMFTSVRVIRLSSLNLTNQALNKNFNDLCENLLKSLYFKLRSMIPC CLCHVNFTVSLPEDELIQVTVTAVAITFDKNQALQTTKTPVEKSLQRASTDNEELLQFPLELCSDSLPS HPFPPAKEHLESASSNSGIPAAQRATSVDYSSFADRCSSWIELIKLKAQTIRRGSIKTTMTVEKASPVG DGNFRNRSAPPCANSTVGVVKMTPLSFIPGAKITKYLGIINMFFIRETTSLREEGGVSGFLHAFIAEVF AMVRAHVAALGGNAVVSYIMKQCVFMENPNKNQAQCLINVSGDAVVFVRESDLEVVSSQQPTTNCQSSC TEGEVTT TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001286177 |
ORF Size | 3126 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reference Data | |
RefSeq | NM_001286177.1, NP_001273106.1 |
RefSeq Size | 4599 bp |
RefSeq ORF | 3129 bp |
Locus ID | 9847 |
UniProt ID | Q86YS7 |
Cytogenetics | 12p12.1 |
MW | 115.4 kDa |
Gene Summary | Required for insulin-stimulated glucose transport and glucose transporter SLC2A4/GLUT4 translocation from intracellular glucose storage vesicle (GSV) to the plasma membrane (PM) in adipocytes. Binds phospholipid membranes in a calcium-dependent manner and is necessary for the optimal membrane fusion between SLC2A4/GLUT4 GSV and the PM.[UniProtKB/Swiss-Prot Function] |
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