CNOT6 (NM_001303241) Human Tagged ORF Clone

CAT#: RG238889

CNOT6 (tGFP-tagged) - Human CCR4-NOT transcription complex, subunit 6 (CNOT6)

Product Images

Circular map for RG238889

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CNOT6
• Synonyms: CCR4; Ccr4a
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG238889 representing NM_001303241.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCCCAAAGAAAAATACGAGCCCCCTGACCCTCGGAGGATGTATACAATTATGTCTTCTGAGGAAGCA
  GCAAATGGAAAGAAATCCCACTGGGCAGAGCTTGAAATAAGTGGAAAAGTAAGAAGCTTAAGCGCATCT
  TTGTGGTCACTAACTCACCTGACAGCTTTGCATTTGAGTGACAATTCCCTGTCCCGAATTCCTTCAGAC
  ATTGCCAAGCTTCACAATCTGGTGTATTTGGACCTGTCATCTAATAAAATTCGTAGCTTACCCGCAGAA
  CTCGGAAACATGGTATCACTCAGGGAGCTCCATTTAAATAACAACCTGTTACGAGTTCTACCTTTTGAG
  CTGGGAAAACTGTTTCAGTTGCAGACTTTAGGCCTGAAAGGAAATCCCCTTACCCAGGATATATTGAAC
  CTTTATCAGGAACCAGATGGAACAAGACGGCTGCTGAACTATTTGCTTGATAATTTGTCAGGTACTGCA
  AAAAGAATTACAACAGAACAACCACCTCCAAGGTCTTGGATTATGTTACAAGAACCAGATAGGACAAGG
  CCAACTGCCTTGTTTTCTGTCATGTGCTATAATGTTCTTTGTGATAAATATGCGACCCGGCAGTTATAC
  GGCTACTGTCCATCATGGGCGCTAAACTGGGACTACAGGAAAAAGGCCATTATTCAAGAAATCTTGAGC
  TGCAATGCTGATATCGTAAGTCTTCAGGAGGTTGAAACGGAACAGTATTACAGTTTTTTTCTGGTAGAG
  CTGAAAGAACGTGGCTATAATGGATTCTTCAGTCCTAAGTCTAGAGCTAGGACAATGTCAGAACAAGAA
  AGGAAACATGTTGATGGCTGTGCAATATTCTTCAAGACAGAAAAATTTACTTTGGTTCAGAAACACACT
  GTTGAATTTAATCAGCTAGCCATGGCAAATTCTGAGGGGTCTGAAGCTATGCTGAACAGAGTCATGACA
  AAAGATAACATTGGGGTTGCAGTACTGCTAGAACTTCGGAAGGAATCGATTGAAATGCCGTCCGGAAAG
  CCACATCTTGGAACAGAAAAACAACTTATTCTTGTGGCTAACGCCCACATGCATTGGGACCCTGAATAC
  TCTGATGTGAAGTTGGTACAAACTATGATGTTCCTCTCAGAAGTGAAGAACATTATTGATAAAGCCTCT
  CGCAACCTCAAATCCAGTGTTTTGGGAGAATTTGGAACTATTCCACTTGTGTTATGTGCAGATCTTAAT
  TCTTTGCCAGACTCTGGTGTTGTAGAATATTTGAGCACAGGTGGAGTAGAAACAAATCACAAAGACTTT
  AAGGAGTTGAGGTATAATGAAAGTCTCACAAACTTCAGCTGTCATGGGAAGAATGGAACCACCAATGGA
  AGGATCACTCATGGTTTCAAGTTACAGAGTGCCTATGAGAGTGGCCTGATGCCTTACACGAATTACACA
  TTTGATTTCAAGGGTATAATAGACTACATTTTCTATTCTAAACCTCAGCTGAACACCTTAGGCATCCTG
  GGCCCTCTGGACCACCACTGGCTGGTTGAGAATAACATCAGTGGCTGCCCGCACCCCCTCATCCCCTCT
  GACCACTTCTCACTTTTTGCACAACTGGAGCTCTTACTGCCTTTCCTGCCCCAAGTCAACGGCATCCAC
  CTTCCTGGCAGGAGG
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG238889
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MPKEKYEPPDPRRMYTIMSSEEAANGKKSHWAELEISGKVRSLSASLWSLTHLTALHLSDNSLSRIPSD
  IAKLHNLVYLDLSSNKIRSLPAELGNMVSLRELHLNNNLLRVLPFELGKLFQLQTLGLKGNPLTQDILN
  LYQEPDGTRRLLNYLLDNLSGTAKRITTEQPPPRSWIMLQEPDRTRPTALFSVMCYNVLCDKYATRQLY
  GYCPSWALNWDYRKKAIIQEILSCNADIVSLQEVETEQYYSFFLVELKERGYNGFFSPKSRARTMSEQE
  RKHVDGCAIFFKTEKFTLVQKHTVEFNQLAMANSEGSEAMLNRVMTKDNIGVAVLLELRKESIEMPSGK
  PHLGTEKQLILVANAHMHWDPEYSDVKLVQTMMFLSEVKNIIDKASRNLKSSVLGEFGTIPLVLCADLN
  SLPDSGVVEYLSTGGVETNHKDFKELRYNESLTNFSCHGKNGTTNGRITHGFKLQSAYESGLMPYTNYT
  FDFKGIIDYIFYSKPQLNTLGILGPLDHHWLVENNISGCPHPLIPSDHFSLFAQLELLLPFLPQVNGIH
  LPGRR
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001303241
• ORF Size: 1671 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).

Reference Data

• RefSeq: NM_001303241.2
• RefSeq Size: 6347 bp
• RefSeq ORF: 1674 bp
• Locus ID: 57472
• Cytogenetics: 5q35.3
• Protein Families: Transcription Factors
• Protein Pathways: RNA degradation
• MW: 63.8 kDa
• Gene Summary: This gene encodes the catalytic component of the CCR4-NOT core transcriptional regulation complex. The encoded protein has a 3'-5' RNase activity and prefers polyadenylated substrates. The CCR4-NOT complex plays a role in many cellular processes, including miRNA-mediated repression, mRNA degradation, and transcriptional regulation. [provided by RefSeq, Dec 2014]