PNLDC1 (NM_001271862) Human Tagged ORF Clone

CAT#: RG238745

PNLDC1 (tGFP-tagged) - Human poly(A)-specific ribonuclease (PARN)-like domain containing 1 (PNLDC1), transcript variant 1

Product Images

Circular map for RG238745

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PNLDC1
• Synonyms: HsPNLDC1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG238745 representing NM_001271862.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGACGTGGGCGCCGACGAGTTCGAGGAGAGCCTCCCTCTGCTGCAGGAGCTCGTCCAGGAGGCCGAC
  TTCGTGGGTCTGGACATAGAGTTCACGGGCCTTCGTTCTAACCTGTCTGGGCCCCAGCAGATCAGTCTT
  TTTGATTTGCCATCGGAGTGGTATCTAAAGACCCGTCAGAGTGTTCAGCAATTTACAGTCTGTCAGATT
  GGATTGTCTGTGTTTTCCGCTATTGAAGGAGAGGCAAACAAGTATATAGCCCATTCTTGTAACTTCTAT
  CTCTTCCCTACAACGTTTGGGATTTTGGACTCAGAATTCTCCTTCCAGGCTTCCAGTGTTCAGTTTTTG
  AATCAGTATGGCTTCAACTATAACAAGTTTCTCAAAAACGGAATCCCATATATGAATGAAGAACAGGAG
  AAGAAAATTAGACACGATATCCTGACTGGGAACTGGAGAGTTCGCAGCTCTCCGGATAAAGACCAAATC
  AAGGTGGTGATTGACGAAGTGACGCGGTGGCTGGAGCTGGCCAAGGAAGGCGACTGGATGACTCTTCCT
  GGGATCACTGGCTTCCAGGCCTTTGAGGTCCAACTGGTGCTGAGGCAGGCCCTCCCCAACATCTGGACG
  GTGCTGAAAGATGAGGGGGTGGTAGTGAAGAAAGTGAGTAAACAACATCGTTGGTATCTTCAGAACACC
  TCTTGTGACCGAGAGAGCTGTTGGAAGGAAAATATTCTTCTCTCAGCAAGGGGTTTTTCTGTCTTTTTC
  CAAATGCTGGTGAAAGCCCAGAAGCCCTTAGTGGGACATAATATGATGATGGACCTGCTGCACCTCCAT
  GAGAAGTTCTTCAGACCCCTCCCAGAAAGCTACGATCAATTTAAGCAGAATATCCACAGCCTATTTCCT
  GTTCTCATTGATACCAAGAGTGTAACAAAGGATATCTGGAAGGAGATGAATTTCCCGAGGGTGTCGAAT
  CTTTCGGAAGTCTATGAAGTCCTGAACAGTGACTTGAATCCCACCAAGAATTCTGGACCAGAGATTGTT
  CACGCGAGCAGGTGTGAGAAATATGTTGAGACAAAGTGCCCCCACGAAGCCGCGTATGATGCCTTCCTC
  TGTGGGTCAGTTCTTTTGAAAGTGGCACACTTGCTTCTACAGAAGATATACCACATCGACCCCGTGCCC
  GAGTCATCCTTTCCTCAGTACCTTGACGTGCTGGCTCCTTACGTGAACCAAGTGAACCTCATCCGAGCG
  GGGGTCCCAAAGATCAATTTTTCTGGTCCAGATTATCCCAGTATCCGACCTCCCATCCTCATCCTCAGC
  GTCAAAAGGTGGCCTGGGGTCAGCGAGCAGCAAGTCTACCATAAGTTTCAGAATCTCTGCAAGTTTGAT
  GTCAGGCGACTCACAAGAAGCCAGTTCTTACTCCTGACCAATAAGTTTAAGGATGCGCGGAACATCCTG
  AAGGAGTACCGGGACCACCCGACCCTGTGCATCTCCCTGTACCGCTACTGGAGGCACTCCCCAAACGTC
  AACTGCCTGCTCCAAGTCTGTGGCATAGTGACTGCCTGGGCCCTTCTCGCGTTCATCCTTGGAAGATCT
  GGTACC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG238745
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MDVGADEFEESLPLLQELVQEADFVGLDIEFTGLRSNLSGPQQISLFDLPSEWYLKTRQSVQQFTVCQI
  GLSVFSAIEGEANKYIAHSCNFYLFPTTFGILDSEFSFQASSVQFLNQYGFNYNKFLKNGIPYMNEEQE
  KKIRHDILTGNWRVRSSPDKDQIKVVIDEVTRWLELAKEGDWMTLPGITGFQAFEVQLVLRQALPNIWT
  VLKDEGVVVKKVSKQHRWYLQNTSCDRESCWKENILLSARGFSVFFQMLVKAQKPLVGHNMMMDLLHLH
  EKFFRPLPESYDQFKQNIHSLFPVLIDTKSVTKDIWKEMNFPRVSNLSEVYEVLNSDLNPTKNSGPEIV
  HASRCEKYVETKCPHEAAYDAFLCGSVLLKVAHLLLQKIYHIDPVPESSFPQYLDVLAPYVNQVNLIRA
  GVPKINFSGPDYPSIRPPILILSVKRWPGVSEQQVYHKFQNLCKFDVRRLTRSQFLLLTNKFKDARNIL
  KEYRDHPTLCISLYRYWRHSPNVNCLLQVCGIVTAWALLAFILGRSGT
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001271862
• ORF Size: 1593 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).

Reference Data

• RefSeq: NM_001271862.2
• RefSeq Size: 1859 bp
• RefSeq ORF: 1596 bp
• Locus ID: 154197
• UniProt ID: Q8NA58
• Cytogenetics: 6q25.3
• MW: 61.8 kDa
• Gene Summary: 3'-exoribonuclease that has a preference for poly(A) tails of mRNAs, thereby efficiently degrading poly(A) tails (PubMed:27515512). Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs and is also used to silence certain maternal mRNAs translationally during oocyte maturation and early embryonic development (PubMed:27515512). May act as a regulator of multipotency in embryonic stem cells (By similarity).[UniProtKB/Swiss-Prot Function]