PTPN5 (NM_001278239) Human Tagged ORF Clone

CAT#: RG238630

PTPN5 (tGFP-tagged) - Human protein tyrosine phosphatase, non-receptor type 5 (striatum-enriched) (PTPN5), transcript variant 6

Product Images

Circular map for RG238630

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PTPN5
• Synonyms: PTPSTEP; STEP; STEP61
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG238630 representing NM_001278239.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTGCTGCAGTGAGAGGCTACCGGGTCTCCCCCAGCCGATAGTGATGGAGGCACTGGACGAGGCTGAA
  GGGCTCCAGGACTCACAGAGAGAGATGCCGCCACCCCCTCCTCCCTCGCCGCCCTCAGATCCAGCTCAG
  AAGCCACCACCTCGAGGCGCTGGGAGCCACTCCCTCACTGTCAGGAGCAGCCTGTGCCTGTTCGCTGCC
  TCACAGTTCCTGCTGGAACCCACGGCCTGGCTTGACTCTGGGACGTGGGGAGTCCCCAGTCTGCTGCTG
  GTCTTTCTGTCCGTGGGCCTGGTCCTCGTTACCACCCTGGTGTGGCACCTCCTGAGGACACCCCCAGAG
  CCACCCACCCCACTGCCCCCTGAGGACAGGCGCCAGTCAGTGAGCCGCCAGCCCTCCTTCACCTACTCA
  GAGTGGATGGAGGAGAAGATCGAGGATGACTTCCTGGACCTCGACCCGGTGCCCGAGACTCCTGTGTTT
  GATTGTGTGATGGACATCAAGCCTGAGGCTGACCCCACCTCACTCACCGTCAAGTCCATGGGTCTGCAG
  GAGAGGAGGGGTTCCAATGTCTCCCTGACCCTGGACATGTGCACTCCGGGCTGCAACGAGGAGGGCTTT
  GGCTATCTCATGTCCCCACGTGAGGAGTCCGCCCGCGAGTACCTGCTCAGCGCCTCCCGTGTCCTCCAA
  GCAGAAGAGCTTCATGAAAAGGCCCTGGACCCTTTCCTGCTGCAGGCGGAATTCTTTGAAATCCCCATG
  AACTTTGTGGATCCGAAAGAGTACGACATCCCTGGGCTGGTGCGGAAGAACCGGTACAAAACCATACTT
  CCCAACCCTCACAGCAGAGTGTGTCTGACCTCACCAGACCCTGACGACCCTCTGAGTTCCTACATCAAT
  GCCAACTACATCCGGGGCTATGGTGGGGAGGAGAAGGTGTACATCGCCACTCAGGGACCCATCGTCAGC
  ACGGTCGCCGACTTCTGGCGCATGGTGTGGCAGGAGCACACGCCCATCATTGTCATGATCACCAACATC
  GAGGAGATGAACGAGAAATGCACCGAGTATTGGCCGGAGGAGCAGGTGGCGTACGACGGTGTTGAGATC
  ACTGTGCAGAAAGTCATTCACACGGAGGATTACCGGCTGCGACTCATCTCCCTCAAGAGTGGGACTGAG
  GAGCGAGGCCTGAAGCATTACTGGTTCACATCCTGGCCCGACCAGAAGACCCCAGACCGGGCCCCCCCA
  CTCCTGCACCTGGTGCGGGAGGTGGAGGAGGCAGCCCAGCAGGAGGGGCCCCACTGTGCCCCCATCATC
  GTCCACTGCAGTGCAGGGATTGGGAGGACCGGCTGCTTCATTGCCACCAGCATCTGCTGCCAGCAGCTG
  CGGCAGGAGGGTGTGGTGGACATCCTGAAGACCACGTGCCAGCTCCGTCAGGACAGGGGCGGCATGATC
  CAGACATGCGAGCAGTACCAGTTTGTGCACCACGTCATGAGCCTCTACGAAAAGCAGCTGTCCCACCAG
  TCCCCAGAA
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG238630
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MCCSERLPGLPQPIVMEALDEAEGLQDSQREMPPPPPPSPPSDPAQKPPPRGAGSHSLTVRSSLCLFAA
  SQFLLEPTAWLDSGTWGVPSLLLVFLSVGLVLVTTLVWHLLRTPPEPPTPLPPEDRRQSVSRQPSFTYS
  EWMEEKIEDDFLDLDPVPETPVFDCVMDIKPEADPTSLTVKSMGLQERRGSNVSLTLDMCTPGCNEEGF
  GYLMSPREESAREYLLSASRVLQAEELHEKALDPFLLQAEFFEIPMNFVDPKEYDIPGLVRKNRYKTIL
  PNPHSRVCLTSPDPDDPLSSYINANYIRGYGGEEKVYIATQGPIVSTVADFWRMVWQEHTPIIVMITNI
  EEMNEKCTEYWPEEQVAYDGVEITVQKVIHTEDYRLRLISLKSGTEERGLKHYWFTSWPDQKTPDRAPP
  LLHLVREVEEAAQQEGPHCAPIIVHCSAGIGRTGCFIATSICCQQLRQEGVVDILKTTCQLRQDRGGMI
  QTCEQYQFVHHVMSLYEKQLSHQSPE
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001278239
• ORF Size: 1527 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).

Reference Data

• RefSeq: NM_001278239.2
• RefSeq Size: 2972 bp
• RefSeq ORF: 1530 bp
• Locus ID: 84867
• UniProt ID: P54829
• Cytogenetics: 11p15.1
• Protein Families: Druggable Genome, Phosphatase, Transmembrane
• Protein Pathways: MAPK signaling pathway
• MW: 57.9 kDa
• Gene Summary: May regulate the activity of several effector molecules involved in synaptic plasticity and neuronal cell survival, including MAPKs, Src family kinases and NMDA receptors.[UniProtKB/Swiss-Prot Function]