PPWD1 (NM_001278929) Human Tagged ORF Clone

CAT#: RG238525

PPWD1 (tGFP-tagged) - Human peptidylprolyl isomerase domain and WD repeat containing 1 (PPWD1), transcript variant 4

Product Images

Circular map for RG238525

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PPWD1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG238525 representing NM_001278929.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAAGGTGTTTGATGTAGTGAACTTTGACATGATCAACATGCTGAAACTTGGCTATTTTCCTGGACAG
  TGTGAGTGGATCTATTGCCCAGGGGATGCAATTTCTTCAGTTGCTGCTTCCGAAAAGAGTACAGGAAAA
  ATTTTCATTTATGATGGCCGAGGAGATAACCAGCCACTTCATATTTTTGACAAACTCCATACATCACCT
  CTTACTCAGATACGGCTAAACCCAGTTTACAAAGCAGTAGTGTCTTCTGACAAATCTGGGATGATTGAA
  TACTGGACTGGGCCTCCTCATGAATATAAATTCCCCAAAAATGTGAACTGGGAATATAAAACTGACACT
  GATTTATATGAATTTGCCAAGTGTAAGGCTTATCCAACCAGCGTATGTTTTTCACCAGATGGGAAGAAA
  ATAGCTACTATTGGTTCTGATAGAAAAGTTAGAATTTTCAGATTTGTAACTGGAAAACTCATGAGAGTC
  TTTGATGAATCACTAAGCATGTTTACTGAACTGCAACAGATGAGGCAACAGTTACCAGACATGGAATTT
  GGCCGACGAATGGCTGTAGAACGTGAGTTGGAGAAGGTTGATGCTGTAAGATTAATTAATATAGTTTTT
  GATGAAACTGGACACTTCGTGCTGTATGGAACAATGCTGGGCATTAAAGTTATAAATGTAGAGACAAAC
  CGGTGTGTGCGGATTTTAGGCAAACAAGAAAATATTAGAGTGATGCAATTGGCTTTGTTCCAGGGGATA
  GCCAAAAAGCATCGTGCTGCAACTACTATAGAAATGAAAGCTTCTGAAAATCCTGTTCTTCAGAATATT
  CAAGCTGACCCAACAATAGTCTGTACATCATTCAAAAAGAATAGATTTTATATGTTTACCAAACGAGAA
  CCAGAAGATACGAAAAGTGCAGATTCTGATCGAGATGTTTTTAATGAGAAACCTTCTAAAGAAGAAGTC
  ATGGCAGCTACTCAAGCTGAAGGACCTAAACGAGTTTCGGACAGTGCCATTATCCACACCAGCATGGGA
  GACATTCACACCAAACTTTTTCCTGTTGAGTGCCCTAAGACAGTGGAAAACTTCTGTGTTCACAGCAGA
  AATGGTTATTATAATGGGCATACATTTCACCGTATAATTAAGGGCTTTATGATTCAGACTGGAGATCCA
  ACAGGTACTGGTATGGGAGGAGAAAGCATATGGGGAGGAGAATTTGAAGATGAATTTCATTCAACATTA
  CGACATGACAGGCCATACACACTCAGCATGGCTAACGCGGGATCAAATACTAATGGATCCCAGTTTTTC
  ATAACGGTAGTACCAACGCCTTGGCTTGATAATAAGCATACAGTATTTGGACGAGTGACTAAAGGAATG
  GAAGTTGTACAGAGGATCTCCAACGTCAAAGTCAATCCCAAAACAGATAAGCCCTATGAGGATGTCAGC
  ATCATAAATATTACTGTCAAG
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG238525
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MKVFDVVNFDMINMLKLGYFPGQCEWIYCPGDAISSVAASEKSTGKIFIYDGRGDNQPLHIFDKLHTSP
  LTQIRLNPVYKAVVSSDKSGMIEYWTGPPHEYKFPKNVNWEYKTDTDLYEFAKCKAYPTSVCFSPDGKK
  IATIGSDRKVRIFRFVTGKLMRVFDESLSMFTELQQMRQQLPDMEFGRRMAVERELEKVDAVRLINIVF
  DETGHFVLYGTMLGIKVINVETNRCVRILGKQENIRVMQLALFQGIAKKHRAATTIEMKASENPVLQNI
  QADPTIVCTSFKKNRFYMFTKREPEDTKSADSDRDVFNEKPSKEEVMAATQAEGPKRVSDSAIIHTSMG
  DIHTKLFPVECPKTVENFCVHSRNGYYNGHTFHRIIKGFMIQTGDPTGTGMGGESIWGGEFEDEFHSTL
  RHDRPYTLSMANAGSNTNGSQFFITVVPTPWLDNKHTVFGRVTKGMEVVQRISNVKVNPKTDKPYEDVS
  IINITVK
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001278929
• ORF Size: 1470 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).

Reference Data

• RefSeq: NM_001278929.1, NP_001265858.1
• RefSeq Size: 2076 bp
• RefSeq ORF: 1473 bp
• Locus ID: 23398
• UniProt ID: Q96BP3
• Cytogenetics: 5q12.3
• MW: 56.1 kDa
• Gene Summary: PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding (PubMed:20676357). May be involved in pre-mRNA splicing (PubMed:11991638).[UniProtKB/Swiss-Prot Function]