RGS20 (NM_001286673) Human Tagged ORF Clone

CAT#: RG237079

RGS20 (tGFP-tagged) - Human regulator of G-protein signaling 20 (RGS20), transcript variant 3

Product Images

Circular map for RG237079

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: RGS20
• Synonyms: g(z)GAP; gz-GAP; RGSZ1; ZGAP1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG237079 representing NM_001286673.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCCCCAGCTTTCCCAAGATAACCAAGAGTGCCTCCAGAAACATTTCTCCAGGCCGTCTATATGGACA
  CAGTTTCTGCCCCTGTTCAGGGCTCAGAGATATAATACAGACATTCACCAAATCACAGAAAATGAAGGA
  GACCTCAGGGCTGTTCCTGATATCAAGCAGATGGGATCAGAGCGGATGGAGATGCGGAAGCGGCAGATG
  CCCGCCGCCCAGGACACACCAGGCGCCGCCCCAGGCCAGCCCGGAGCGGGGAGTCGCGGGTCCAACGCA
  TGCTGCTTCTGCTGGTGCTGCTGTTGTAGCTGCTCGTGTCTCACTGTTAGAAACCAGGAAGATCAGAGG
  CCCACAATAGCTTCCCACGAACTCAGAGCAGATCTTCCAACCTGGGAAGAAAGCCCTGCTCCTACTCTG
  GAAGAAGTCAACGCCTGGGCTCAGTCATTTGACAAATTAATGGTCACTCCAGCAGGAAGGAATGCATTC
  CGTGAATTCCTCCGAACAGAATTCAGTGAGGAAAATATGCTCTTCTGGATGGCCTGTGAGGAACTGAAA
  AAGGAAGCTAATAAAAACATTATTGAAGAGAAAGCAAGGATAATCTATGAAGACTACATTTCTATACTT
  TCTCCTAAGGAGGTGAGCTTAGACTCCCGGGTGAGAGAAGTGATCAACAGAAACATGGTGGAGCCATCC
  CAACACATATTCGATGATGCTCAACTTCAGATTTACACCCTGATGCACAGAGACTCATATCCTCGATTC
  ATGAACTCTGCTGTCTATAAGGACTTGCTTCAGTCCTTATCGGAGAAATCTATTGAAGCA
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG237079
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MPQLSQDNQECLQKHFSRPSIWTQFLPLFRAQRYNTDIHQITENEGDLRAVPDIKQMGSERMEMRKRQM
  PAAQDTPGAAPGQPGAGSRGSNACCFCWCCCCSCSCLTVRNQEDQRPTIASHELRADLPTWEESPAPTL
  EEVNAWAQSFDKLMVTPAGRNAFREFLRTEFSEENMLFWMACEELKKEANKNIIEEKARIIYEDYISIL
  SPKEVSLDSRVREVINRNMVEPSQHIFDDAQLQIYTLMHRDSYPRFMNSAVYKDLLQSLSEKSIEA
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001286673
• ORF Size: 819 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).

Reference Data

• RefSeq: NM_001286673.2
• RefSeq Size: 1760 bp
• RefSeq ORF: 822 bp
• Locus ID: 8601
• UniProt ID: O76081
• Cytogenetics: 8q11.23
• Protein Families: Druggable Genome
• MW: 31.9 kDa
• Gene Summary: The protein encoded by this gene belongs to the family of regulator of G protein signaling (RGS) proteins, which are regulatory and structural components of G protein-coupled receptor complexes. RGS proteins inhibit signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound forms. This protein selectively binds to G(z)-alpha and G(alpha)-i2 subunits, and regulates their signaling activities. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]