NCE2 (UBE2F) (NM_001278306) Human Tagged ORF Clone

CAT#: RG236184

UBE2F (tGFP-tagged) - Human ubiquitin-conjugating enzyme E2F (putative) (UBE2F), transcript variant 3

Product Images

Circular map for RG236184

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: NCE2
• Synonyms: NCE2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG236184 representing NM_001278306.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGTGTTGGGCGCCGGGCCCGCCTCGCCTGTCTCGGGGAGCCCAGAGGTTGCAGAACTTGAAGCTAAT
  TTACCTTGTACATGTAAAGTGCATTTTCCTGATCCAAACAAGCTTCATTGTTTTCAGCTAACAGTAACC
  CCAGATGAGGGTTACTACCAGGGTGGAAAATTTCAGTTTGAAACTGAAGTTCCCGATGCGTACAACATG
  GTGCCTCCCAAAGTGAAATGCCTGACCAAGATCTGGCACCCCAACATCACAGAGACAGGGGAAATATGT
  CTGAGTTTATTGAGAGAACATTCAATTGATGGCACTGGCTGGGCTCCCACAAGAACATTAAAGGATGTC
  GTTTGGGGATTAAACTCTTTGTTTACTGATCTTTTGAATTTTGATGATCCACTGAATATTGAAGCTGCA
  GAACATCATTTGCGGGACAAGGAGGACTTCCGGAATAAAGTGGATGACTACATCAAACGTTATGCCAGA
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG236184
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MVLGAGPASPVSGSPEVAELEANLPCTCKVHFPDPNKLHCFQLTVTPDEGYYQGGKFQFETEVPDAYNM
  VPPKVKCLTKIWHPNITETGEICLSLLREHSIDGTGWAPTRTLKDVVWGLNSLFTDLLNFDDPLNIEAA
  EHHLRDKEDFRNKVDDYIKRYAR
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001278306
• ORF Size: 483 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).

Reference Data

• RefSeq: NM_001278306.1, NP_001265235.1
• RefSeq Size: 2072 bp
• RefSeq ORF: 486 bp
• Locus ID: 140739
• UniProt ID: Q969M7
• Cytogenetics: 2q37.3
• Protein Pathways: Ubiquitin mediated proteolysis
• MW: 18.6 kDa
• Gene Summary: Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.[UniProtKB/Swiss-Prot Function]