KIF1A (NM_001244008) Human Tagged ORF Clone

CAT#: RG235336

KIF1A (tGFP-tagged) - Homo sapiens kinesin family member 1A (KIF1A), transcript variant 1

Product Images

Circular map for RG235336

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: KIF1A
• Synonyms: ATSV; C2orf20; HSN2C; MRD9; NESCAVS; SPG30; UNC104
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG235336 representing NM_001244008
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGGGGCTTCGGTGAAGGTGGCGGTGCGGGTCCGCCCCTTCAATTCCCGGGAAATGAGCCGTGACT
  CCAAGTGCATCATTCAGATGTCTGGAAGCACCACCACCATTGTTAACCCCAAACAGCCCAAGGAGACGCC
  CAAAAGCTTCAGCTTTGACTACTCCTACTGGTCGCACACCTCACCTGAGGACATCAACTACGCGTCGCAG
  AAGCAGGTGTACCGGGACATCGGCGAGGAGATGCTGCAGCATGCCTTTGAGGGATACAACGTGTGCATCT
  TCGCCTATGGGCAGACGGGTGCCGGCAAGTCCTACACCATGATGGGCAAGCAGGAGAAGGACCAGCAGGG
  CATCATCCCACAGCTCTGCGAGGACCTCTTCTCTCGGATCAACGACACGACCAACGACAACATGTCCTAC
  TCCGTGGAGGTCAGCTACATGGAGATTTACTGTGAGCGCGTCCGTGACCTCCTGAACCCCAAGAACAAGG
  GCAACCTTCGCGTGAGGGAGCACCCACTGCTGGGGCCCTACGTGGAGGACCTCTCCAAGCTGGCTGTCAC
  CTCCTACAATGACATCCAGGACCTCATGGACTCAGGGAACAAGGCCAGGACCGTGGCGGCCACCAACATG
  AATGAGACCAGCAGTCGCTCCCACGCCGTCTTCAACATCATCTTCACCCAGAAGCGCCATGACGCAGAGA
  CCAATATCACCACGGAGAAGGTGAGCAAAATCAGCCTGGTGGACCTGGCTGGGAGCGAGCGGGCTGACTC
  CACGGGAGCCAAGGGCACGCGCCTCAAGGAGGGGGCCAACATCAACAAGTCGCTGACCACCCTGGGCAAG
  GTCATCTCCGCCCTGGCTGAAATGGACTCCGGACCCAACAAGAACAAGAAAAAGAAGAAGACAGATTTCA
  TTCCGTACCGAGATTCCGTGTTGACCTGGCTCCTCCGGGAAAACCTGGGCGGTAACTCAAGGACAGCTAT
  GGTGGCAGCCTTGAGTCCTGCAGACATCAACTACGATGAGACCCTTAGCACGCTGAGGTATGCTGACCGG
  GCCAAGCAGATCCGCTGCAATGCTGTCATCAATGAGGACCCCAACAACAAGCTGATCCGCGAGCTGAAGG
  ATGAGGTGACCCGGCTGCGGGACCTTCTGTACGCCCAGGGTCTTGGCGACATCACTGACACCAACACTGT
  GCCTGGAGGACCCAAATTGACCAATGCCCTGGTGGGTATGAGCCCCTCATCCTCGCTCTCAGCCCTGTCC
  AGCCGCGCGGCCTCCGTGTCCAGCCTCCACGAGCGCATCTTGTTTGCCCCGGGCAGCGAGGAGGCCATTG
  AAAGACTGAAGGAAACAGAGAAGATCATAGCTGAGCTCAATGAGACCTGGGAGGAGAAGCTGCGGCGGAC
  AGAAGCCATCCGGATGGAGAGGGAAGCCCTGCTGGCCGAGATGGGTGTGGCCATGAGGGAGGATGGCGGC
  ACCTTGGGCGTATTCTCTCCCAAAAAGACACCACACCTCGTCAACCTGAACGAGGACCCGCTGATGTCTG
  AGTGCCTGCTCTACTACATCAAGGATGGGATCACCAGAGTGGGCAGGGAGGATGGCGAGAGGCGGCAGGA
  CATTGTTCTGAGTGGGCACTTCATCAAGGAGGAGCACTGCGTCTTCCGGAGCGACTCCAGGGGAGGCAGC
  GAAGCTGTGGTGACCTTGGAGCCCTGTGAGGGGGCAGACACCTACGTCAATGGCAAGAAAGTCACAGAGC
  CCAGCATCCTGCGTTCAGGAAACCGCATCATCATGGGTAAGAGCCATGTGTTCCGGTTCAACCACCCCGA
  GCAGGCCCGGCAGGAGCGTGAGCGCACGCCTTGTGCGGAGACGCCAGCTGAGCCTGTGGACTGGGCCTTC
  GCCCAGCGTGAGCTGCTGGAGAAGCAGGGCATCGACATGAAGCAGGAGATGGAGCAGAGGCTCCAGGAAC
  TGGAGGACCAGTACCGCCGCGAGCGGGAGGAGGCCACCTACCTGCTGGAGCAGCAGCGGCTGGACTATGA
  GAGCAAGCTGGAGGCTCTGCAGAAGCAGATGGACTCCAGGTACTACCCGGAGGTGAACGAGGAGGAGGAG
  GAGCCCGAGGATGAAGTCCAGTGGACAGAGCGGGAGTGTGAGCTGGCGCTCTGGGCCTTCCGGAAGTGGA
  AGTGGTACCAGTTCACGTCTCTGCGGGACCTGCTGTGGGGCAACGCCATCTTCCTCAAGGAGGCCAATGC
  CATCAGCGTGGAGCTGAAAAAGAAGGTACAATTCCAGTTTGTCCTCCTGACGGACACACTCTACTCCCCT
  CTGCCACCCGACCTGCTGCCCCCAGAGGCCGCCAAAGACCGAGAGACGCGGCCCTTCCCCCGCACCATTG
  TGGCCGTGGAGGTCCAGGACCAGAAGAACGGGGCCACCCACTACTGGACGCTGGAGAAGCTCAGGCAGCG
  TCTGGACCTGATGCGGGAGATGTACGACCGCGCTGCAGAGGTGCCCTCCAGTGTCATCGAGGACTGTGAC
  AACGTGGTGACCGGCGGAGACCCCTTCTATGACCGCTTCCCCTGGTTCCGGCTGGTGGGCAGTTCAGCCA
  TCTCTGGCTGCAACAGCTACCCTCTTCTCAACACATGCATGAGCGAGCGCATGGCTGCTCTCACCCCCTC
  CCCCACCTTCTCGAGCCCCGACTCCGACGCCACCGAGCCTGCCGAGGAGCAGAGCGTGGGGGAGGAGGAG
  GAGGAGGAGGAGGAGGAGGAGGATGAGGAGGAGGAGGACCTGGAGGACGACGTCTTTCCGGAGCACGCGC
  TGTGCGACGGCCGGGACCCGTTTTACGACCGGCCCCCCCTGTTCAGTTTAGTAGGAAGGGCCTTCGTGTA
  CCTGAGCAACCTGCTGTACCCCGTTCCCCTGGTACACCGTGTGGCAATCGTCAGCGAGAAGGGCGAGGTG
  AAGGGCTTCCTCCGCGTGGCCGTCCAGGCCATCTCAGCCGATGAAGAGGCCCCTGATTATGGCTCTGGCG
  TCCGCCAGTCGGGAACTGCTAAAATCTCCTTTGATGACCAGCATTTTGAAAAGTTCCAGTCCGAGTCTTG
  CCCCGTGGTGGGGATGTCCCGCTCGGGAACCTCCCAGGAAGAGCTTCGCATCGTGGAGGGCCAGGGCCAG
  GGTGCAGACGTGGGGCCCTCAGCCGATGAAGTCAACAACAACACCTGTTCAGCAGTGCCCCCAGAAGGCC
  TCCTCCTAGACAGCTCTGAGAAAGCCGCCCTGGATGGGCCCCTGGATGCTGCCCTGGACCACCTCCGCCT
  GGGCAACACCTTCACCTTCCGTGTGACAGTCCTGCAGGCGTCCAGCATCTCTGCCGAATATGCCGACATC
  TTCTGCCAGTTCAACTTCATCCACCGCCACGACGAGGCCTTCTCCACAGAGCCCCTGAAGAACACAGGCA
  GAGGCCCCCCACTTGGCTTCTACCACGTCCAGAACATCGCAGTGGAGGTGACCAAGTCCTTCATTGAGTA
  CATCAAGAGCCAGCCCATTGTTTTCGAGGTCTTTGGCCACTACCAGCAGCACCCGTTCCCGCCCCTCTGC
  AAGGACGTGCTCAGCCCCCTGAGGCCCTCGCGCCGCCACTTCCCTCGGGTCATGCCACTGTCCAAGCCAG
  TGCCCGCCACCAAGCTCAGCACACTGACGCGGCCCTGTCCGGGACCCTGCCACTGCAAGTACGACCTGCT
  GGTCTACTTCGAGATCTGTGAGCTGGAGGCCAACGGCGATTACATCCCGGCCGTGGTGGACCACCGTGGG
  GGCATGCCATGCATGGGGACCTTCCTCCTCCACCAGGGCATCCAGCGACGGATTACGGTGACACTACTGC
  ATGAGACAGGCAGCCATATCCGCTGGAAGGAAGTGCGCGAGCTGGTCGTGGGCCGCATCCGAAACACTCC
  AGAGACCGACGAGTCCCTGATCGACCCCAACATCTTGTCTCTCAACATCCTCTCTTCCGGATACATCCAC
  CCAGCCCAAGATGACCGGACCTTTTACCAATTTGAGGCTGCGTGGGACAGCTCCATGCACAACTCTCTCC
  TGCTGAACCGGGTCACCCCTTATCGAGAGAAAATCTACATGACACTCTCCGCTTATATCGAGATGGAGAA
  CTGCACCCAGCCGGCTGTTGTCACCAAGGACTTCTGCATGGTCTTCTATTCCCGTGATGCCAAGCTGCCA
  GCCTCGCGCTCCATCCGCAACCTCTTTGGCAGTGGGAGCCTTCGGGCCTCAGAGAGTAACCGTGTGACTG
  GTGTGTACGAGCTCAGCCTGTGCCACGTGGCTGACGCGGGCAGCCCAGGGATGCAGCGCCGGCGCCGACG
  AGTCCTGGACACATCTGTGGCCTATGTCCGGGGCGAGGAGAACCTGGCAGGCTGGAGGCCCCGGAGTGAC
  AGTCTCATTCTGGACCACCAGTGGGAGCTGGAGAAGCTGAGCCTCCTGCAGGAGGTGGAGAAGACTAGGC
  ACTACCTGCTCCTGCGGGAGAAGCTGGAGACCGCCCAGCGGCCTGTCCCGGAGGCACTGTCCCCGGCCTT
  CAGCGAGGACTCTGAGTCCCATGGCTCCTCCAGCGCCTCCTCCCCGCTCTCGGCTGAGGGCCGCCCATCA
  CCCCTGGAGGCTCCCAACGAGAGGCAGCGGGAGCTGGCCGTCAAGTGCTTGCGCCTGCTCACGCACACAT
  TCAACAGAGAGTACACACACAGCCACGTCTGCGTCAGTGCCAGCGAGAGCAAGCTCTCCGAGATGTCTGT
  CACCCTGCTCCGGGACCCGTCGATGTCCCCTCTAGGGGTGGCCACTCTCACCCCCTCCTCCACTTGCCCC
  TCTCTGGTTGAAGGGCGGTACGGTGCCACTGACCTGAGGACCCCGCAGCCCTGCTCCCGGCCAGCCAGCC
  CAGAGCCCGAGCTGCTGCCAGAGGCCGACTCCAAGAAGCTCCCTTCCCCTGCCCGGGCAACAGAGACAGA
  CAAGGAGCCCCAGCGCCTGCTGGTCCCTGACATCCAGGAGATCCGAGTCAGCCCGATCGTTTCCAAGAAG
  GGGTACCTGCACTTCCTGGAGCCGCACACGTCAGGCTGGGCCAGGCGCTTCGTGGTGGTGCGGCGCCCCT
  ATGCCTACATGTACAACAGCGACAAGGACACCGTGGAGCGGTTCGTGCTCAACCTGGCCACTGCCCAGGT
  GGAGTACAGTGAGGACCAGCAGGCTATGCTCAAGACACCCAACACATTCGCGGTGTGCACGGAACACCGC
  GGCATCCTGCTGCAGGCCGCCAGCGACAAGGACATGCATGACTGGCTGTACGCCTTCAACCCCCTCCTGG
  CCGGGACCATACGGTCCAAGCTCTCCAGAAGGAGGTCTGCCCAGATGCGGGTC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG235336 representing NM_001244008
  Red=Cloning site Green=Tags(s)
  
  MAGASVKVAVRVRPFNSREMSRDSKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQ
  KQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSY
  SVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNM
  NETSSRSHAVFNIIFTQKRHDAETNITTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGK
  VISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADR
  AKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDTNTVPGGPKLTNALVGMSPSSSLSALS
  SRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGG
  TLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDGERRQDIVLSGHFIKEEHCVFRSDSRGGS
  EAVVTLEPCEGADTYVNGKKVTEPSILRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAF
  AQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEE
  EPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSP
  LPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSVIEDCD
  NVVTGGDPFYDRFPWFRLVGSSAISGCNSYPLLNTCMSERMAALTPSPTFSSPDSDATEPAEEQSVGEEE
  EEEEEEEDEEEEDLEDDVFPEHALCDGRDPFYDRPPLFSLVGRAFVYLSNLLYPVPLVHRVAIVSEKGEV
  KGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFDDQHFEKFQSESCPVVGMSRSGTSQEELRIVEGQGQ
  GADVGPSADEVNNNTCSAVPPEGLLLDSSEKAALDGPLDAALDHLRLGNTFTFRVTVLQASSISAEYADI
  FCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTKSFIEYIKSQPIVFEVFGHYQQHPFPPLC
  KDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLTRPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRG
  GMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLNILSSGYIH
  PAQDDRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVVTKDFCMVFYSRDAKLP
  ASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSD
  SLILDHQWELEKLSLLQEVEKTRHYLLLREKLETAQRPVPEALSPAFSEDSESHGSSSASSPLSAEGRPS
  PLEAPNERQRELAVKCLRLLTHTFNREYTHSHVCVSASESKLSEMSVTLLRDPSMSPLGVATLTPSSTCP
  SLVEGRYGATDLRTPQPCSRPASPEPELLPEADSKKLPSPARATETDKEPQRLLVPDIQEIRVSPIVSKK
  GYLHFLEPHTSGWARRFVVVRRPYAYMYNSDKDTVERFVLNLATAQVEYSEDQQAMLKTPNTFAVCTEHR
  GILLQAASDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001244008
• ORF Size: 5373 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001244008.2
• RefSeq Size: 9233 bp
• RefSeq ORF: 5376 bp
• Locus ID: 547
• UniProt ID: Q12756
• Cytogenetics: 2q37.3
• Protein Families: Druggable Genome
• Gene Summary: The protein encoded by this gene is a member of the kinesin family and functions as an anterograde motor protein that transports membranous organelles along axonal microtubules. Mutations at this locus have been associated with spastic paraplegia-30 and hereditary sensory neuropathy IIC. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Apr 2012]