USP19 (NM_001199160) Human Tagged ORF Clone
CAT#: RG235299
- TrueORF®
USP19 (tGFP-tagged) - Homo sapiens ubiquitin specific peptidase 19 (USP19), transcript variant 1
ORF Plasmid: DDK
"NM_001199160" in other vectors (2)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | USP19 |
Synonyms | ZMYND9 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG235299 representing NM_001199160
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGGCGGGGCCAGTGCCACAGGCCCAAGGAGAGGGCCCCCAGGACTGGAGGACACCACTAGTAAGA AGAAGCAGAAGGATCGAGCAAACCAGGAGAGCAAGGATGGAGATCCTAGGAAAGAGACAGGGTCTCGATA TGTTGCCCAGGCTGGTCTTGAACCTCTGGCCTCAGGTGATCCTTCTGCCTCAGCCTCCCATGCAGCTGGG ATCACAGGCTCACGCCACCGTACCCGGCTGTTCTTTCCTTCATCGTCAGGGTCAGCATCCACTCCTCAAG AGGAGCAGACCAAAGAGGGAGCTTGTGAAGACCCTCATGATCTCTTGGCTACTCCCACTCCAGAGTTGTT GCTCGATTGGAGGCAGAGTGCAGAAGAGGTGATTGTCAAGCTTCGTGTGGGAGTAGGTCCCCTGCAGCTG GAGGATGTAGATGCTGCTTTCACAGATACAGACTGTGTGGTGCGGTTTGCAGGTGGTCAGCAGTGGGGTG GTGTCTTCTATGCTGAGATAAAAAGCTCTTGTGCTAAAGTGCAAACCCGCAAGGGCAGTCTCCTGCACCT GACACTGCCCAAAAAGGTGCCTATGCTCACGTGGCCCTCCCTCCTGAAGAAACCTCTAGGGACCCAGGAG CTGGTGCCGGGGCTGCGGTGCCAGGAGAATGGGCAGGAACTGTCTCCCATTGCCCTGGAGCCAGGCCCTG AGCCCCACCGGGCTAAGCAGGAGGCCCGGAACCAGAAGCGGGCCCAGGGCCGTGGTGAGGTAGGCGCAGG GGCTGGCCCCGGGGCCCAGGCAGGGCCCAGCGCCAAGAGGGCTGTGCATCTCTGCAGAGGGCCAGAGGGG GACGGGTCCAGGGATGACCCTGGACCCCGGGGTGATGCCCCACCCTTCGTGGCTGACCCAGCCACCCAGG TTGAGGCTGATGAACAGCTTTGCATACCACCGCTGAACTCCCAAACCTGCCTCCTGGGCTCAGAGGAGAA TTTAGCCCCTTTGGCAGGAGAGAAAGCAGTGCCTCCCGGGAATGACCCAGTCTCTCCAGCCATGGTCCGG AGCAGAAACCCTGGGAAAGATGACTGTGCCAAGGAGGAGATGGCAGTGGCAGCAGATGCTGCAACCTTGG TGGATGAGCCCGAGTCGATGGTGAACCTGGCGTTTGTCAAGAATGACTCGTATGAGAAGGGCCCGGATTC AGTGGTGGTGCACGTGTACGTGAAGGAGATCTGCAGGGACACCTCAAGAGTACTTTTCCGTGAGCAGGAC TTCACGCTCATCTTCCAGACCAGGGATGGAAACTTCCTGAGGCTGCACCCGGGCTGTGGGCCCCACACCA CCTTCCGTTGGCAGGTGAAGCTCAGGAATCTGATTGAGCCAGAGCAGTGCACCTTCTGTTTCACGGCTTC TCGCATCGACATCTGCCTTCGTAAGAGGCAGAGTCAGCGCTGGGGGGGCCTGGAGGCCCCGGCTGCACGA GTGGGTGGTGCAAAGGTTGCCGTGCCGACAGGTCCAACCCCTCTGGATTCAACCCCACCAGGAGGTGCTC CCCACCCCCTGACAGGCCAGGAGGAGGCCCGGGCTGTGGAGAAGGATAAATCCAAGGCACGATCTGAGGA CACAGGGCTAGACAGTGTGGCAACCCGCACACCCATGGAGCATGTAACCCCAAAGCCAGAGACACACCTG GCCTCGCCCAAGCCTACATGCATGGTGCCTCCCATGCCCCACAGCCCAGTTAGTGGAGACAGCGTGGAGG AGGAGGAAGAGGAAGAGAAGAAGGTGTGTCTGCCAGGCTTCACTGGCCTTGTCAATTTAGGCAACACCTG CTTCATGAACAGCGTCATTCAGTCTCTGTCCAACACTCGGGAACTCCGGGACTTCTTCCATGACCGCTCC TTTGAGGCTGAGATCAACTACAACAACCCACTAGGGACTGGTGGGCGTCTGGCCATTGGCTTTGCCGTGC TGCTTCGGGCGCTGTGGAAGGGCACCCACCATGCCTTCCAGCCTTCCAAGTTGAAGGCCATTGTGGCGAG TAAGGCCAGCCAGTTCACAGGCTATGCACAGCATGATGCCCAGGAGTTCATGGCTTTCCTGCTGGATGGG CTGCACGAGGACCTGAATCGCATTCAGAACAAGCCCTACACAGAGACCGTGGATTCAGATGGGCGGCCCG ATGAGGTGGTAGCTGAGGAAGCATGGCAGCGGCACAAGATGAGGAATGACTCTTTCATCGTGGACCTATT TCAGGGGCAGTACAAGTCGAAGCTGGTGTGCCCTGTGTGTGCCAAGGTCTCCATCACTTTTGACCCGTTT CTTTATCTGCCGGTGCCCTTGCCACAAAAGCAAAAGGTTCTCCCTGTCTTTTATTTTGCCCGAGAGCCCC ACAGCAAGCCCATCAAGTTCCTGGTGAGCGTCAGCAAGGAGAACTCCACTGCGAGCGAAGTATTGGACTC CCTCTCTCAGAGTGTTCATGTGAAGCCTGAGAACCTGCGTTTGGCGGAGGTAATTAAGAATCGTTTTCAT CGTGTGTTCCTACCCTCCCACTCACTGGACACTGTGTCCCCATCTGATACGCTCCTCTGCTTTGAGCTGC TATCCTCAGAGTTGGCTAAGGAGCGGGTAGTGGTGCTAGAGGTGCAACAGCGCCCCCAGGTGCCCAGCGT CCCCATCTCCAAGTGTGCAGCCTGCCAGCGGAAGCAACAGTCGGAGGATGAAAAGCTGAAGCGCTGTACC CGGTGCTACCGTGTGGGCTACTGCAACCAGCTCTGCCAGAAAACCCACTGGCCTGACCACAAGGGCCTCT GCCGACCTGAGAACATTGGCTACCCCTTCCTGGTCAGTGTACCTGCCTCACGCCTCACTTATGCCCGCCT CGCTCAGTTGCTAGAGGGCTATGCCCGGTACTCTGTGAGTGTATTCCAGCCACCCTTTCAGCCAGGCCGC ATGGCCTTGGAGTCTCAGAGCCCTGGCTGCACCACACTGCTCTCCACAGGTTCCCTGGAGGCTGGGGACA GCGAGAGAGACCCCATTCAGCCACCTGAGCTCCAGCTGGTGACCCCTATGGCTGAGGGGGACACAGGGCT TCCCCGGGTGTGGGCAGCCCCTGACCGGGGTCCTGTGCCCAGCACCAGTGGAATTTCTTCTGAGATGCTG GCCAGTGGGCCCATTGAGGTTGGCTCCTTGCCAGCTGGCGAGAGGGTGTCCCGACCCGAAGCTGCTGTGC CTGGGTACCAGCATCCAAGTGAAGCTATGAATGCCCACACACCCCAGTTCTTCATCTATAAAATTGATTC ATCCAACCGAGAGCAGCGGCTAGAGGACAAAGGAGACACCCCACTGGAGCTGGGTGACGACTGTAGCCTG GCTCTCGTCTGGCGGAACAATGAGCGCTTGCAGGAGTTTGTGTTGGTAGCCTCCAAGGAGCTGGAATGTG CTGAGGATCCAGGCTCTGCCGGTGAGGCTGCCCGGGCCGGCCACTTCACCCTGGACCAGTGCCTCAACCT CTTCACACGGCCTGAGGTGCTGGCACCCGAGGAGGCCTGGTACTGCCCACAGTGCAAACAGCACCGTGAG GCCTCCAAGCAGCTGTTGCTATGGCGCCTGCCAAATGTTCTCATCGTGCAGCTCAAGCGCTTCTCCTTTC GTAGTTTTATCTGGCGTGACAAGATCAATGACTTGGTGGAGTTCCCTGTTAGGAACCTGGACCTGAGCAA GTTCTGCATTGGTCAGAAAGAGGAGCAGCTGCCCAGCTACGATCTATATGCTGTCATCAACCACTATGGA GGCATGATTGGTGGCCACTACACTGCCTGTGCACGCCTGCCCAATGATCGTAGCAGTCAGCGCAGTGACG TGGGCTGGCGCTTGTTTGATGACAGCACAGTGACAACGGTAGACGAGAGCCAGGTTGTGACGCGTTATGC CTATGTACTCTTCTACCGCCGGCGGAACTCTCCTGTGGAGAGGCCCCCCAGGGCAGGTCACTCTGAGCAC CACCCAGACCTAGGCCCTGCAGCTGAGGCTGCTGCCAGCCAGGCTTCCCGGATTTGGCAGGAGCTGGAGG CTGAGGAGGAGCCGGTGCCTGAGGGGTCTGGGCCCCTGGGTCCCTGGGGGCCCCAAGACTGGGTGGGCCC CCTACCACGTGGCCCTACCACACCAGATGAGGGCTGCCTCCGGTACTTTGTCCTGGGCACCGTGGCGGCT TTGGTGGCCCTCGTGCTCAACGTGTTCTATCCTCTGGTATCCCAGAGTCGCTGGAGA AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG235299 representing NM_001199160
Red=Cloning site Green=Tags(s) MSGGASATGPRRGPPGLEDTTSKKKQKDRANQESKDGDPRKETGSRYVAQAGLEPLASGDPSASASHAAG ITGSRHRTRLFFPSSSGSASTPQEEQTKEGACEDPHDLLATPTPELLLDWRQSAEEVIVKLRVGVGPLQL EDVDAAFTDTDCVVRFAGGQQWGGVFYAEIKSSCAKVQTRKGSLLHLTLPKKVPMLTWPSLLKKPLGTQE LVPGLRCQENGQELSPIALEPGPEPHRAKQEARNQKRAQGRGEVGAGAGPGAQAGPSAKRAVHLCRGPEG DGSRDDPGPRGDAPPFVADPATQVEADEQLCIPPLNSQTCLLGSEENLAPLAGEKAVPPGNDPVSPAMVR SRNPGKDDCAKEEMAVAADAATLVDEPESMVNLAFVKNDSYEKGPDSVVVHVYVKEICRDTSRVLFREQD FTLIFQTRDGNFLRLHPGCGPHTTFRWQVKLRNLIEPEQCTFCFTASRIDICLRKRQSQRWGGLEAPAAR VGGAKVAVPTGPTPLDSTPPGGAPHPLTGQEEARAVEKDKSKARSEDTGLDSVATRTPMEHVTPKPETHL ASPKPTCMVPPMPHSPVSGDSVEEEEEEEKKVCLPGFTGLVNLGNTCFMNSVIQSLSNTRELRDFFHDRS FEAEINYNNPLGTGGRLAIGFAVLLRALWKGTHHAFQPSKLKAIVASKASQFTGYAQHDAQEFMAFLLDG LHEDLNRIQNKPYTETVDSDGRPDEVVAEEAWQRHKMRNDSFIVDLFQGQYKSKLVCPVCAKVSITFDPF LYLPVPLPQKQKVLPVFYFAREPHSKPIKFLVSVSKENSTASEVLDSLSQSVHVKPENLRLAEVIKNRFH RVFLPSHSLDTVSPSDTLLCFELLSSELAKERVVVLEVQQRPQVPSVPISKCAACQRKQQSEDEKLKRCT RCYRVGYCNQLCQKTHWPDHKGLCRPENIGYPFLVSVPASRLTYARLAQLLEGYARYSVSVFQPPFQPGR MALESQSPGCTTLLSTGSLEAGDSERDPIQPPELQLVTPMAEGDTGLPRVWAAPDRGPVPSTSGISSEML ASGPIEVGSLPAGERVSRPEAAVPGYQHPSEAMNAHTPQFFIYKIDSSNREQRLEDKGDTPLELGDDCSL ALVWRNNERLQEFVLVASKELECAEDPGSAGEAARAGHFTLDQCLNLFTRPEVLAPEEAWYCPQCKQHRE ASKQLLLWRLPNVLIVQLKRFSFRSFIWRDKINDLVEFPVRNLDLSKFCIGQKEEQLPSYDLYAVINHYG GMIGGHYTACARLPNDRSSQRSDVGWRLFDDSTVTTVDESQVVTRYAYVLFYRRRNSPVERPPRAGHSEH HPDLGPAAEAAASQASRIWQELEAEEEPVPEGSGPLGPWGPQDWVGPLPRGPTTPDEGCLRYFVLGTVAA LVALVLNVFYPLVSQSRWR SGPTRTRRLE - GFP Tag - V |
Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001199160 |
ORF Size | 4257 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001199160.1, NP_001186089.1 |
RefSeq Size | 4878 bp |
RefSeq ORF | 4260 bp |
Locus ID | 10869 |
UniProt ID | O94966 |
Cytogenetics | 3p21.31 |
Protein Families | Druggable Genome, Protease, Transmembrane |
Gene Summary | Protein ubiquitination controls many intracellular processes, including cell cycle progression, transcriptional activation, and signal transduction. This dynamic process, involving ubiquitin conjugating enzymes and deubiquitinating enzymes, adds and removes ubiquitin. Deubiquitinating enzymes are cysteine proteases that specifically cleave ubiquitin from ubiquitin-conjugated protein substrates. This protein is a ubiquitin protein ligase and plays a role in muscle wasting. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2017] |
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