Attractin (ATRN) (NM_001207047) Human Tagged ORF Clone

CAT#: RG235195

ATRN (tGFP-tagged) - Homo sapiens attractin (ATRN), transcript variant 4

Product Images

Circular map for RG235195

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Attractin
• Synonyms: DPPT-L; MGCA
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG235195 representing NM_001207047
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGTTCGCGGGGCGCCGTCTCAGCCCCGGGAAGATGGTGGCTGCAGCGGCGGCAACTGAGACTAACTG
  GATCTTCTGGGTTTGTGACAGATGGACCTGGAAATTATAAATACAAAACGAAGTGCACGTGGCTCATTGA
  AGGACAGCCAAATAGAATAATGAGACTTCGTTTCAATCATTTTGCTACAGAGTGTAGTTGGGACCATTTA
  TATGTTTATGATGGGGACTCAATTTATGCACCGCTAGTTGCTGCATTTAGTGGCCTCATTGTTCCTGAGA
  GAGATGGCAATGAGACTGTCCCTGAGGTTGTTGCCACATCAGGTTATGCCTTGCTGCATTTTTTTAGTGA
  TGCTGCTTATAATTTGACTGGATTTAATATTACTTACAGTTTTGATATGTGTCCAAATAACTGCTCAGGC
  CGAGGAGAGTGTAAGATCAGTAATAGCAGCGATACTGTTGAATGTGAATGTTCTGAAAACTGGAAAGGTG
  AAGCATGTGACATTCCTCACTGTACAGACAACTGTGGTTTTCCTCATCGAGGCATCTGCAATTCAAGTGA
  TGTCAGAGGATGCTCCTGCTTCTCAGACTGGCAGGGTCCTGGATGTTCAGTTCCTGTACCAGCTAACCAG
  TCATTTTGGACTCGAGAGGAATATTCTAACTTAAAGCTCCCCAGAGCATCTCATAAAGCTGTGGTCAATG
  GAAACATTATGTGGGTTGTTGGAGGATATATGTTCAACCACTCAGATTATAACATGGTTCTAGCGTATGA
  CCTTGCTTCTAGGGAGTGGCTTCCACTAAACCGTTCTGTGAACAATGTGGTTGTTAGATATGGTCATTCT
  TTGGCATTATACAAGGATAAAATTTACATGTATGGAGGAAAAATTGATTCAACTGGGAATGTGACCAATG
  AGTTGAGAGTTTTTCACATTCATAATGAGTCATGGGTGTTGTTGACCCCTAAGGCAAAGGAGCAGTATGC
  AGTGGTTGGGCACTCTGCACACATTGTTACACTGAAGAATGGCCGAGTGGTCATGCTGGTCATCTTTGGT
  CACTGCCCTCTCTATGGATATATAAGCAATGTGCAGGAATATGATTTGGATAAGAACACATGGAGTATAT
  TACACACCCAGGGTGCCCTTGTGCAAGGGGGTTACGGCCATAGCAGTGTTTACGACCATAGGACCAGGGC
  CCTATACGTTCATGGTGGCTACAAGGCTTTCAGTGCCAATAAGTACCGGCTTGCAGATGATCTCTACCGA
  TATGATGTGGATACCCAGATGTGGACCATTCTTAAGGACAGCCGATTTTTCCGTTACTTGCACACAGCTG
  TGATAGTGAGTGGAACCATGCTGGTGTTTGGAGGAAACACACACAATGACACATCTATGAGCCATGGCGC
  CAAATGCTTCTCTTCAGATTTCATGGCCTATGACATTGCCTGTGACCGCTGGTCAGTGCTTCCCAGACCT
  GATCTCCACCATGATGTCAACAGATTTGGCCATTCAGCAGTCTTACACAACAGCACCATGTATGTGTTCG
  GTGGTTTCAATAGTCTCCTCCTCAGCGACATCCTGGTATTCACCTCGGAACAGTGTGATGCGCATCGGAG
  TGAAGCCGCTTGTTTAGCAGCAGGACCTGGTATTCGGTGTGTGTGGAACACAGGGTCGTCTCAGTGTATC
  TCGTGGGCGCTGGCAACTGATGAACAAGAAGAAAAGTTAAAATCAGAATGTTTTTCCAAAAGAACTCTTG
  ACCATGACAGATGTGACCAGCACACAGATTGTTACAGCTGCACAGCCAACACCAATGACTGCCACTGGTG
  CAATGACCATTGTGTCCCCAGGAACCACAGCTGCTCAGAAGGCCAGATCTCCATTTTTAGGTATGAGAAT
  TGCCCCAAGGATAACCCCATGTACTACTGTAACAAGAAGACCAGCTGCAGGAGCTGTGCCCTGGACCAGA
  ACTGCCAGTGGGAGCCCCGGAATCAGGAGTGCATTGCCCTGCCCGAAAATATCTGTGGCATTGGCTGGCA
  TTTGGTTGGAAACTCATGTTTGAAAATTACTACTGCCAAGGAGAATTATGACAATGCTAAATTGTTCTGT
  AGGAACCACAATGCCCTTTTGGCTTCTCTTACAACCCAGAAGAAGGTAGAATTTGTCCTTAAGCAGCTGC
  GAATAATGCAGTCATCTCAGAGCATGTCCAAGCTCACCTTAACCCCATGGGTCGGCCTTCGGAAGATCAA
  TGTGTCCTACTGGTGCTGGGAAGATATGTCCCCATTTACAAATAGTTTACTACAGTGGATGCCGTCTGAG
  CCCAGTGATGCTGGATTCTGTGGAATTTTATCAGAACCCAGTACTCGGGGACTGAAGGCTGCAACCTGCA
  TCAACCCACTCAATGGTAGTGTCTGTGAAAGGCCTGCAAACCACAGTGCTAAGCAGTGCCGGACACCATG
  TGCCTTGAGGACAGCATGTGGAGATTGCACCAGCGGCAGCTCTGAGTGCATGTGGTGCAGCAACATGAAG
  CAGTGTGTGGACTCCAATGCCTACGTGGCCTCCTTCCCTTTTGGCCAGTGTATGGAATGGTATACGATGA
  GCACCTGCCCCCCTGAAAATTGTTCAGGCTACTGTACCTGTAGTCATTGCTTGGAGCAACCAGGCTGTGG
  CTGGTGTACTGATCCCAGCAATACTGGCAAAGGGAAATGCATAGAGGGTTCCTATAAAGGACCAGTGAAG
  ATGCCTTCGCAAGCCCCTACAGGAAATTTCTATCCACAGCCCCTGCTCAATTCCAGCATGTGTCTAGAGG
  ACAGCAGATACAACTGGTCTTTCATTCACTGTCCAGCTTGCCAATGCAACGGCCACAGTAAATGCATCAA
  TCAGAGCATCTGTGAGAAGTGTGAGAACCTGACCACAGGCAAGCACTGCGAGACCTGCATATCTGGCTTC
  TACGGTGATCCCACCAATGGAGGGAAATGTCAGCCATGCAAGTGCAATGGGCACGCGTCTCTGTGCAACA
  CCAACACGGGCAAGTGCTTCTGCACCACCAAGGGCGTCAAGGGGGACGAGTGCCAGCTATGTGAGGTAGA
  AAATCGATACCAAGGAAACCCTCTCAGAGGAACATGTTATTATACTCTTCTTATTGACTATCAGTTCACC
  TTTAGTCTATCCCAGGAAGATGATCGCTATTACACAGCTATCAATTTTGTGGCTACTCCTGACGAACAAA
  ACAGGGATTTGGACATGTTCATCAATGCCTCCAAGAATTTCAACCTCAACATCACCTGGGCTGCCAGTTT
  CTCAGCTGGAACCCAGGCTGGAGAAGAGATGCCTGTTGTTTCAAAAACCAACATTAAGGAGTACAAAGAT
  AGTTTCTCTAATGAGAAGTTTGATTTTCGCAACCACCCAAATATCACTTTCTTTGTTTATGTCAGTAATT
  TCACCTGGCCCATCAAAATTCAGGTGCAAACTGAACAA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG235195 representing NM_001207047
  Red=Cloning site Green=Tags(s)
  
  MCSRGAVSAPGRWWLQRRQLRLTGSSGFVTDGPGNYKYKTKCTWLIEGQPNRIMRLRFNHFATECSWDHL
  YVYDGDSIYAPLVAAFSGLIVPERDGNETVPEVVATSGYALLHFFSDAAYNLTGFNITYSFDMCPNNCSG
  RGECKISNSSDTVECECSENWKGEACDIPHCTDNCGFPHRGICNSSDVRGCSCFSDWQGPGCSVPVPANQ
  SFWTREEYSNLKLPRASHKAVVNGNIMWVVGGYMFNHSDYNMVLAYDLASREWLPLNRSVNNVVVRYGHS
  LALYKDKIYMYGGKIDSTGNVTNELRVFHIHNESWVLLTPKAKEQYAVVGHSAHIVTLKNGRVVMLVIFG
  HCPLYGYISNVQEYDLDKNTWSILHTQGALVQGGYGHSSVYDHRTRALYVHGGYKAFSANKYRLADDLYR
  YDVDTQMWTILKDSRFFRYLHTAVIVSGTMLVFGGNTHNDTSMSHGAKCFSSDFMAYDIACDRWSVLPRP
  DLHHDVNRFGHSAVLHNSTMYVFGGFNSLLLSDILVFTSEQCDAHRSEAACLAAGPGIRCVWNTGSSQCI
  SWALATDEQEEKLKSECFSKRTLDHDRCDQHTDCYSCTANTNDCHWCNDHCVPRNHSCSEGQISIFRYEN
  CPKDNPMYYCNKKTSCRSCALDQNCQWEPRNQECIALPENICGIGWHLVGNSCLKITTAKENYDNAKLFC
  RNHNALLASLTTQKKVEFVLKQLRIMQSSQSMSKLTLTPWVGLRKINVSYWCWEDMSPFTNSLLQWMPSE
  PSDAGFCGILSEPSTRGLKAATCINPLNGSVCERPANHSAKQCRTPCALRTACGDCTSGSSECMWCSNMK
  QCVDSNAYVASFPFGQCMEWYTMSTCPPENCSGYCTCSHCLEQPGCGWCTDPSNTGKGKCIEGSYKGPVK
  MPSQAPTGNFYPQPLLNSSMCLEDSRYNWSFIHCPACQCNGHSKCINQSICEKCENLTTGKHCETCISGF
  YGDPTNGGKCQPCKCNGHASLCNTNTGKCFCTTKGVKGDECQLCEVENRYQGNPLRGTCYYTLLIDYQFT
  FSLSQEDDRYYTAINFVATPDEQNRDLDMFINASKNFNLNITWAASFSAGTQAGEEMPVVSKTNIKEYKD
  SFSNEKFDFRNHPNITFFVYVSNFTWPIKIQVQTEQ
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001207047
• ORF Size: 3468 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001207047.3
• RefSeq Size: 3584 bp
• RefSeq ORF: 3471 bp
• Locus ID: 8455
• UniProt ID: O75882
• Cytogenetics: 20p13
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Gene Summary: This gene encodes both membrane-bound and secreted protein isoforms. A membrane-bound isoform exhibits sequence similarity with the mouse mahogany protein, a receptor involved in controlling obesity. A secreted isoform is involved in the initial immune cell clustering during inflammatory responses that may regulate the chemotactic activity of chemokines. [provided by RefSeq, Apr 2016]