Liprin alpha 2 (PPFIA2) (NM_001220478) Human Tagged ORF Clone

CAT#: RG235191

PPFIA2 (tGFP-tagged) - Homo sapiens protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 2 (PPFIA2), transcript variant 7

Product Images

Circular map for RG235191

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Liprin alpha 2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG235191 representing NM_001220478
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTTTCTCGGATATGAACACCGTTTCTGGCTCCCCTAAAGTGCATCCTCCTAATGGGACCCGGTTTT
  ACACTTTTCAAGAATTTGCTGCACTGACAAAAGAATTAAATGCCTGCAGGGAACAACTTCTAGAAAAGGA
  AGAAGAAATCTCTGAACTTAAAGCTGAAAGAAACAACACAAGACTATTACTGGAGCATTTGGAGTGCCTT
  GTGTCACGACATGAAAGATCACTAAGAATGACGGTGGTAAAACGGCAAGCCCAGTCTCCCTCAGGAGTAT
  CCAGTGAAGTTGAAGTTCTCAAGGCACTGAAATCTTTGTTTGAGCACCACAAGGCCTTGGATGAAAAGAT
  TGTTGCCTTGCGTGAACAAAATGTTCATATACAAAGAAAAATGGCATCAAGCGAGGGATCCACAGAGTCA
  GAACATCTTGAAGGGATGGAACCTGGACAGAAAGTCCATGAGAAGCGTTTGTCCAATGGTTCTATAGACT
  CAACCGATGAAACTAGTCAAATAGTTGAACTACAAGAATTGCTTGAAAAGCAAAACTATGAAATGGCCCA
  GATGAAAGAACGTTTAGCAGCCCTTTCTTCCCGAGTGGGAGAGGTGGAACAGGAAGCAGAGACAGCAAGA
  AAGGATCTCATTAAAACAGAAGAAATGAACACCAAGTATCAAAGGGACATTAGGGAGGCCATGGCACAAA
  AGGAAGATATGGAAGAAAGAATTACAACCCTTGAAAAGCGTTACCTCAGTGCTCAGAGAGAATCTACCTC
  CATACATGACATGAATGATAAACTAGAAAATGAGTTAGCAAATAAAGAAGCTATCCTGCGGCAGATGGAA
  GAGAAAAACAGACAGTTACAAGAACGTCTTGAGCTAGCTGAACAAAAGTTGCAGCAGACCATGAGAAAGG
  CTGAAACCTTGCCTGAAGTAGAGGCTGAACTGGCTCAGAGAATTGCAGCCCTAACCAAGGCTGAAGAGAG
  ACATGGAAATATTGAAGAACGTATGAGACATTTAGAGGGTCAACTTGAAGAGAAGAATCAAGAACTTCAA
  AGAGCTAGGCAAAGAGAGAAAATGAATGAGGAGCATAACAAGAGATTATCGGATACGGTTGATAGACTTC
  TGACTGAATCCAATGAACGCCTACAACTACACTTAAAGGAAAGAATGGCTGCTCTAGAAGAAAAGAATGT
  TTTAATTCAAGAATCAGAAACTTTCAGAAAGAATCTTGAAGAATCTTTACATGATAAGGAAAGATTAGCA
  GAAGAAATTGAAAAGCTGAGATCTGAACTTGACCAATTGAAAATGAGAACTGGCTCTTTAATTGAACCCA
  CAATACCAAGAACTCATCTAGACACCTCAGCTGAGTTGCGGTACTCAGTGGGATCCCTAGTGGACAGCCA
  GTCTGATTACAGAACAACTAAAGTAATAAGAAGACCAAGGAGAGGCCGCATGGGTGTGCGAAGAGATGAG
  CCAAAGGTGAAATCTCTTGGGGATCACGAGTGGAATAGAACTCAACAGATTGGAGTACTAAGCAGCCACC
  CTTTTGAAAGTGACACTGAAATGTCTGATATTGATGATGATGACAGAGAAACAATTTTTAGCTCAATGGA
  TCTTCTCTCTCCAAGTGGTCATTCCGATGCCCAGACGCTAGCCATGATGCTTCAGGAACAATTGGATGCC
  ATCAACAAAGAAATCAGGCTAATTCAGGAAGAAAAAGAATCTACAGAGTTGCGTGCTGAAGAAATTGAAA
  ATAGAGTGGCTAGTGTGAGCCTCGAAGGCCTGAATTTGGCAAGGGTCCACCCAGGTACCTCCATTACTGC
  CTCTGTTACAGCTTCATCGCTGGCCAGTTCATCTCCCCCCAGTGGACACTCAACTCCAAAGCTCACCCCT
  CGAAGCCCTGCCAGGGAAATGGATCGGATGGGAGTCATGACACTGCCAAGTGATCTGAGGAAACATCGGA
  GAAAGATTGCAGTTGTGGAAGAAGATGGTCGAGAGGACAAAGCAACAATTAAATGTGAAACTTCTCCTCC
  TCCTACCCCTAGAGCCCTCAGAATGACTCACACTCTCCCTTCTTCCTACCACAATGATGCTCGAAGTAGT
  TTATCTGTCTCTCTTGAGCCAGAAAGCCTCGGGCTTGGTAGTGCCAACAGCAGCCAAGACTCTCTTCACA
  AAGCCCCCAAGAAGAAAGGAATCAAGTCTTCAATAGGACGTTTGTTTGGTAAAAAAGAAAAAGCTCGACT
  TGGGCAGCTCCGAGGCTTTATGGAGACTGAAGCTGCAGCTCAGGAGTCCCTGGGGTTAGGCAAACTCGGA
  ACTCAAGCTGAGAAGGATCGAAGACTAAAGAAAAAGCATGAACTTCTTGAAGAAGCTCGGAGAAAGGGAT
  TACCTTTTGCCCAGTGGGATGGGCCAACTGTGGTCGCATGGCTAGAGCTTTGGTTGGGAATGCCTGCGTG
  GTACGTGGCAGCCTGCCGAGCCAACGTGAAGAGTGGTGCCATCATGTCTGCTTTATCTGACACTGAGATC
  CAGAGAGAAATTGGAATCAGCAATCCACTGCATCGCTTAAAACTTCGATTAGCAATCCAGGAGATGGTTT
  CCCTAACAAGTCCTTCAGCTCCTCCAACATCTCGAACTCCTTCAGGCAACGTTTGGGTGACTCATGAAGA
  AATGGAAAATCTTGCAGCTCCAGCAAAAACGAAAGAATCTGAGGAAGGAAGCTGGGCCCAGACCCTGGCT
  TATGGAGATATGAATCATGAGTGGATTGGAAATGAATGGCTTCCCAGCTTGGGGTTACCTCAGTACAGAA
  GTTACTTTATGGAATGCTTGGTAGATGCAAGAATGTTAGATCACCTAACAAAAAAAGATCTCCGTGTCCA
  TTTAAAAATGGTGGATAGTTTCCATCGAACAAGTTTACAATATGGAATTATGTGCTTAAAGAGGTTGAAT
  TATGACAGAAAAGAACTAGAAAGAAGACGGGAAGCAAGCCAACATGAAATAAAAGACGTGTTGGTGTGGA
  GCAATGACCGAGTTATTCGCTGGATACAAGCAATTGGACTTCGAGAATATGCAAATAATATACTTGAGAG
  CGGTGTGCATGGCTCACTTATAGCCCTGGATGAAAACTTTGACTACAGCAGCTTAGCTTTATTATTACAG
  ATTCCAACACAGAACACCCAGGCAAGGCAGATTCTTGAAAGAGAATACAATAACCTCTTGGCCCTGGGAA
  CTGAAAGGCGACTGGATGAAAGTGATGACAAGAACTTCAGACGTGGATCAACCTGGAGAAGGCAGTTTCC
  TCCTCGTGAAGTACATGGAATCAGCATGATGCCTGGGTCCTCAGAAACATTACCAGCTGGATTTAGGTTA
  ACCACAACCTCTGGGCAGTCAAGAAAAATGACAACAGATGTTGCTTCATCAAGACTGCAGAGGTTAGACA
  ACTCCACTGTTCGCACATACTCATGT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG235191 representing NM_001220478
  Red=Cloning site Green=Tags(s)
  
  MIFSDMNTVSGSPKVHPPNGTRFYTFQEFAALTKELNACREQLLEKEEEISELKAERNNTRLLLEHLECL
  VSRHERSLRMTVVKRQAQSPSGVSSEVEVLKALKSLFEHHKALDEKIVALREQNVHIQRKMASSEGSTES
  EHLEGMEPGQKVHEKRLSNGSIDSTDETSQIVELQELLEKQNYEMAQMKERLAALSSRVGEVEQEAETAR
  KDLIKTEEMNTKYQRDIREAMAQKEDMEERITTLEKRYLSAQRESTSIHDMNDKLENELANKEAILRQME
  EKNRQLQERLELAEQKLQQTMRKAETLPEVEAELAQRIAALTKAEERHGNIEERMRHLEGQLEEKNQELQ
  RARQREKMNEEHNKRLSDTVDRLLTESNERLQLHLKERMAALEEKNVLIQESETFRKNLEESLHDKERLA
  EEIEKLRSELDQLKMRTGSLIEPTIPRTHLDTSAELRYSVGSLVDSQSDYRTTKVIRRPRRGRMGVRRDE
  PKVKSLGDHEWNRTQQIGVLSSHPFESDTEMSDIDDDDRETIFSSMDLLSPSGHSDAQTLAMMLQEQLDA
  INKEIRLIQEEKESTELRAEEIENRVASVSLEGLNLARVHPGTSITASVTASSLASSSPPSGHSTPKLTP
  RSPAREMDRMGVMTLPSDLRKHRRKIAVVEEDGREDKATIKCETSPPPTPRALRMTHTLPSSYHNDARSS
  LSVSLEPESLGLGSANSSQDSLHKAPKKKGIKSSIGRLFGKKEKARLGQLRGFMETEAAAQESLGLGKLG
  TQAEKDRRLKKKHELLEEARRKGLPFAQWDGPTVVAWLELWLGMPAWYVAACRANVKSGAIMSALSDTEI
  QREIGISNPLHRLKLRLAIQEMVSLTSPSAPPTSRTPSGNVWVTHEEMENLAAPAKTKESEEGSWAQTLA
  YGDMNHEWIGNEWLPSLGLPQYRSYFMECLVDARMLDHLTKKDLRVHLKMVDSFHRTSLQYGIMCLKRLN
  YDRKELERRREASQHEIKDVLVWSNDRVIRWIQAIGLREYANNILESGVHGSLIALDENFDYSSLALLLQ
  IPTQNTQARQILEREYNNLLALGTERRLDESDDKNFRRGSTWRRQFPPREVHGISMMPGSSETLPAGFRL
  TTTSGQSRKMTTDVASSRLQRLDNSTVRTYSC
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001220478
• ORF Size: 3456 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001220478.2, NP_001207407.1
• RefSeq Size: 5517 bp
• RefSeq ORF: 3459 bp
• Locus ID: 8499
• UniProt ID: O75334
• Cytogenetics: 12q21.31
• Protein Families: Druggable Genome
• Gene Summary: The protein encoded by this gene is a member of the LAR protein-tyrosine phosphatase-interacting protein (liprin) family. Liprins interact with members of LAR family of transmembrane protein tyrosine phosphatases, which are known to be important for axon guidance and mammary gland development. It has been proposed that liprins are multivalent proteins that form complex structures and act as scaffolds for the recruitment and anchoring of LAR family of tyrosine phosphatases. This protein has been shown to bind the calcium/calmodulin-dependent serine protein kinase (MAGUK family) protein (also known as CASK) and proposed to regulate higher-order brain functions in mammals. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]