ARHGAP12 (NM_001270697) Human Tagged ORF Clone

CAT#: RG234896

ARHGAP12 (tGFP-tagged) - Homo sapiens Rho GTPase activating protein 12 (ARHGAP12), transcript variant 4

Product Images

Circular map for RG234896

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ARHGAP12
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG234896 representing NM_001270697
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAATGGCTGACAGAAGTGGGAAGATTATTCCAGGACAAGTGTATATTGAGGTGGAATATGATTATG
  AATATGAAGCAAAGGACAGAAAGATTGTGATAAAACAAGGGGAGAGGTACATCTTGGTGAAAAAGACCAA
  TGATGACTGGTGGCAAGTCAAGCCAGATGAAAACTCCAAAGCGTTTTATGTGCCAGCCCAGTATGTGAAG
  GAGGTCACGCGCAAAGCTCTCATGCCACCTGTTAAGCAGGTAGCTGGTCTGCCAAATAACTCCACGAAAA
  TAATGCAGAGTTTGCATCTTCAGAGATCAACAGAAAATGTGAACAAATTGCCTGAGCTTTCAAGTTTCGG
  AAAGCCATCGTCATCTGTTCAAGGAACAGGTCTTATTCGTGATGCCAATCAGAATTTTGGACCCAGTTAT
  AATCAAGGTCAGACTGTCAACCTAAGCCTGGACCTGACCCATAATAACGGAAAGTTTAACAATGACTCAC
  ATTCTCCTAAAGTTTCCAGCCAGAATAGGACACGCTCATTTGGTCATTTTCCCGGTCCAGAGTTCTTGGA
  TGTAGAGAAAACTAGCTTCTCCCAGGAACAATCTTGTGATTCCGCAGGAGAAGGCTCTGAAAGAATACAT
  CAAGATTCTGAATCTGGTGATGAACTTAGCAGCAGCTCCACTGAACAGATAAGGGCAACCACACCTCCAA
  ATCAAGGAAGGCCAGATTCTCCTGTCTATGCTAACCTTCAAGAACTGAAAATATCCCAGTCTGCTCTTCC
  CCCACTTCCTGGGAGCCCGGCAATTCAGATTAATGGAGAATGGGAAACTCATAAAGACAGCTCAGGGCGT
  TGCTATTACTATAACAGAGGGACACAGGAAAGAACTTGGAAACCTCCTCGTTGGACTCGGGATGCAAGCA
  TCAGCAAAGGAGATTTCCAAAATCCAGGGGATCAAGAGTGGCTCAAGCATGTTGATGATCAAGGTAGACA
  ATATTACTACAGTGCAGACGGATCTCGGTCAGAATGGGAATTGCCAAAGTATAATGCTTCATCCCAGCAG
  CAAAGAGAAATAATTAAAAGTAGGAGCCTGGACAGGCGGCTGCAAGAACCAATAGTATTAACAAAGTGGA
  GACATAGCACCATTGTATTGGACACTAATGATAAGGAATCTCCAACTGCCTCAAAACCCTGCTTTCCTGA
  AAATGAGTCTTCTCCCTCCTCACCAAAGCACCAAGATACAGCCAGCAGTCCAAAGGATCAAGAGAAATAT
  GGATTATTAAATGTAACAAAAATTGCTGAAAATGGGAAAAAGGTTCGAAAGAACTGGTTGTCTTCTTGGG
  CGGTGTTGCAGGGTTCATCTTTACTTTTTACCAAAACTCAAGGAAGTAGCACAAGTTGGTTTGGCAGTAA
  TCAGTCCAAACCAGAGTTCACAGTGGACCTCAAGGGGGCAACAATTGAGATGGCTTCAAAGGATAAATCC
  AGCAAAAAGAATGTATTTGAGCTGAAAACTCGTCAAGGAACAGAACTGCTAATTCAGTCTGACAATGACA
  CTGTTATTAATGATTGGTTTAAAGTTCTTAGTAGTACAATCAATAATCAGGCAGTAGAAACTGATGAAGG
  AATTGAAGAGGAGATACCGGATTCACCAGGAATAGAAAAGCATGATAAAGAAAAGGAACAAAAGGATCCC
  AAAAAGCTTCGTTCCTTTAAAGTATCTAGCATAGATTCTTCAGAACAGAAAAAAACCAAGAAAAACTTAA
  AGAAGTTTCTTACACGACGCCCCACTTTGCAAGCTGTTCGTGAAAAAGGTTATATTAAAGATCAGGTATT
  TGGATCCAATCTCGCTAATCTGTGTCAGAGAGAGAATGGCACAGTACCAAAGTTTGTGAAGTTATGTATT
  GAACATGTTGAAGAACATGGTTTGGATATTGATGGGATATACAGAGTAAGTGGCAACCTCGCAGTGATCC
  AGAAACTAAGGTTTGCAGTCAATCATGATGAGAAATTGGACTTGAATGACAGTAAATGGGAAGATATTCA
  TGTCATTACTGGAGCCCTCAAAATGTTTTTTCGAGAATTACCAGAACCTCTTTTTACATTTAATCATTTT
  AATGATTTTGTTAATGCAATTAAGCAAGAACCAAGACAGCGAGTCGCTGCTGTTAAGGACCTAATCAGAC
  AGTTGCCAAAGCCAAACCAAGACACAATGCAGATTCTTTTCCGACATCTCAGAAGAGTTATAGAAAATGG
  AGAGAAAAATCGAATGACCTATCAGAGTATAGCAATTGTTTTTGGTCCCACTCTATTAAAACCAGAAAAA
  GAGACTGGTAATATAGCAGTTCATACTGTGTACCAGAATCAGATTGTAGAATTAATTCTTCTGGAACTGA
  GTTCCATCTTCGGACGT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG234896 representing NM_001270697
  Red=Cloning site Green=Tags(s)
  
  MKMADRSGKIIPGQVYIEVEYDYEYEAKDRKIVIKQGERYILVKKTNDDWWQVKPDENSKAFYVPAQYVK
  EVTRKALMPPVKQVAGLPNNSTKIMQSLHLQRSTENVNKLPELSSFGKPSSSVQGTGLIRDANQNFGPSY
  NQGQTVNLSLDLTHNNGKFNNDSHSPKVSSQNRTRSFGHFPGPEFLDVEKTSFSQEQSCDSAGEGSERIH
  QDSESGDELSSSSTEQIRATTPPNQGRPDSPVYANLQELKISQSALPPLPGSPAIQINGEWETHKDSSGR
  CYYYNRGTQERTWKPPRWTRDASISKGDFQNPGDQEWLKHVDDQGRQYYYSADGSRSEWELPKYNASSQQ
  QREIIKSRSLDRRLQEPIVLTKWRHSTIVLDTNDKESPTASKPCFPENESSPSSPKHQDTASSPKDQEKY
  GLLNVTKIAENGKKVRKNWLSSWAVLQGSSLLFTKTQGSSTSWFGSNQSKPEFTVDLKGATIEMASKDKS
  SKKNVFELKTRQGTELLIQSDNDTVINDWFKVLSSTINNQAVETDEGIEEEIPDSPGIEKHDKEKEQKDP
  KKLRSFKVSSIDSSEQKKTKKNLKKFLTRRPTLQAVREKGYIKDQVFGSNLANLCQRENGTVPKFVKLCI
  EHVEEHGLDIDGIYRVSGNLAVIQKLRFAVNHDEKLDLNDSKWEDIHVITGALKMFFRELPEPLFTFNHF
  NDFVNAIKQEPRQRVAAVKDLIRQLPKPNQDTMQILFRHLRRVIENGEKNRMTYQSIAIVFGPTLLKPEK
  ETGNIAVHTVYQNQIVELILLELSSIFGR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001270697
• ORF Size: 2397 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001270697.1, NP_001257626.1
• RefSeq Size: 4964 bp
• RefSeq ORF: 2400 bp
• Locus ID: 94134
• UniProt ID: Q8IWW6
• Cytogenetics: 10p11.22
• Gene Summary: This gene encodes a member of a large family of proteins that activate Rho-type guanosine triphosphate (GTP) metabolizing enzymes. The encoded protein may be involved in suppressing tumor formation by regulating cell invasion and adhesion. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]