ANKRD6 (NM_001242813) Human Tagged ORF Clone

CAT#: RG234727

ANKRD6 (tGFP-tagged) - Homo sapiens ankyrin repeat domain 6 (ANKRD6), transcript variant 4

Product Images

Circular map for RG234727

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ANKRD6
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG234727 representing NM_001242813
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCCAGCAAGATGCGGTCGCTGCACTTTCAGAGCGCCTTCTCGTAGCTGCGTACAAAGGCCAAACAG
  AGAATGTGGTTCAGCTCATCAACAAGGGCGCCAGGGTAGCGGTTACCAAGCATGGCCGGACTCCCCTGCA
  TCTTGCTGCCAATAAGGGCCATCTTCCTGTGGTCCAGATCTTGCTGAAGGCTGGCTGCGACCTTGATGTC
  CAGGATGATGGGGACCAGACCGCCTTGCACCGGGCCACAGTGGTGGGGAACACGGAGATCATCGCGGCGC
  TCATCCACGAAGGGTGTGCCCTGGACAGACAAGACAAGGATGGGAATACAGCCTTGCATGAAGCATCCTG
  GCATGGTTTCAGCCAGTCAGCCAAGCTGCTCATTAAAGCAGGAGCCAACGTGCTTGCCAAGAACAAGGCG
  GGGAACACAGCTCTGCACCTGGCCTGCCAGAACAGCCACTCCCAGAGCACGCGCGTCCTCCTGCTGGCCG
  GGTCCCGCGCTGACCTCAAAAATAATGCAGGAGACACCTGTTTGCACGTTGCTGCGCGCTATAATCACTT
  GTCCATCATTAGGCTCCTCCTCACTGCTTTCTGTTCTGTCCATGAAAAGAACCAGGCTGGAGACACAGCA
  CTTCACGTTGCTGCTGCCCTAAATCACAAGAAGGTGGCCAAAATCTTACTGGAAGCCGGAGCAGATACGA
  CCATTGTTAACAATGCAGGCCAGACTCCGCTGGAGACTGCCCGCTACCACAATAACCCGGAAGTTGCTCT
  TCTCCTTACTAAAGCTCCCCAGGGCAGTGTCTCAGCAGGAGACACCCCCAGCAGTGAACAGGCTGTGGCC
  AGAAAAGAAGAAGCCAGAGAAGAGTTCCTGTCAGCCTCCCCAGAACCCAGAGCAAAGGATGACAGGAGGA
  GAAAGTCAAGGCCCAAGGTGTCAGCATTTTCTGACCCCACCCCACCAGCCGACCAACAGCCTGGACACCA
  GAAGAACCTGCATGCTCATAATCACCCTAAAAAGAGGAACAGGCATCGGTGTTCATCCCCACCCCCACCC
  CATGAGTTCAGGGCGTATCAGCTCTACACATTGTACCGGGGCAAGGATGGGAAAGTGATGCAGGCACCAA
  TAAATGGTTGTCGATGTGAACCTCTAATCAACAAGCTGGAGAATCAGTTGGAGGCTACTGTGGAGGAGAT
  AAAAGCAGAGCTGGGATCGGTTCAGGACAAAATGAATACAAAGCTGGGGCAGATGGAGAATAAGACCCAG
  CACCAAATGCGTGTTTTGGACAAGCTGATGGTTGAGCGACTTTCTGCAGAGAGGACGGAGTGCCTGAACC
  GCCTGCAACAGCACTCAGACACAGAGAAGCATGAGGGGGAGAAACGACAGATATCCTTGGTGGATGAATT
  AAAAACCTGGTGCATGTTAAAGATTCAGAATCTGGAGCAGAAGCTTTCTGGAGATTCTAGGGCCTGCAGA
  GCTAAATCCACACCATCTACTTGTGAGTCCTCTACAGGTGTGGACCAATTAGTGGTGACTGCAGGTCCAG
  CAGCAGCTTCCGACAGCTCCCCTCCAGTGGTTAGGCCCAAAGAGAAGGCCCTCAACTCCACTGCTACCCA
  GAGACTCCAGCAGGAGCTGTCGTCTTCTGACTGTACAGGCTCCCGACTGAGAAACGTCAAGGTCCAGACA
  GCCTTGCTACCCATGAATGAGGCAGCCAGATCTGATCAGCAGGCTGGGCCCTGCGTCAACAGAGGCACTC
  AAACTAAGAAGTCTGGGAAGAGTGGGCCAACAAGGCATCGTGCCCAGCAACCCGCAGCCAGCAGCACCTG
  TGGGCAGCCGCCACCAGCCACAGGCAGCGAGCAGACTGGCCCTCACATTCGGGACACCTCCCAAGCTCTG
  GAGCTTACCCAGTATTTTTTTGAGGCTGTTTCTACCCAGATGGAAAAGTGGTATGAAAGGAAGATTGAAG
  AAGCACGAAGCCAAGCCAATCAGAAAGCCCAGCAAGATAAGGCTACATTGAAGGAACACATTAAAAGTTT
  AGAAGAGGAACTTGCCAAACTAAGGACTAGGGTGCAGAAGGAAAAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG234727 representing NM_001242813
  Red=Cloning site Green=Tags(s)
  
  MSQQDAVAALSERLLVAAYKGQTENVVQLINKGARVAVTKHGRTPLHLAANKGHLPVVQILLKAGCDLDV
  QDDGDQTALHRATVVGNTEIIAALIHEGCALDRQDKDGNTALHEASWHGFSQSAKLLIKAGANVLAKNKA
  GNTALHLACQNSHSQSTRVLLLAGSRADLKNNAGDTCLHVAARYNHLSIIRLLLTAFCSVHEKNQAGDTA
  LHVAAALNHKKVAKILLEAGADTTIVNNAGQTPLETARYHNNPEVALLLTKAPQGSVSAGDTPSSEQAVA
  RKEEAREEFLSASPEPRAKDDRRRKSRPKVSAFSDPTPPADQQPGHQKNLHAHNHPKKRNRHRCSSPPPP
  HEFRAYQLYTLYRGKDGKVMQAPINGCRCEPLINKLENQLEATVEEIKAELGSVQDKMNTKLGQMENKTQ
  HQMRVLDKLMVERLSAERTECLNRLQQHSDTEKHEGEKRQISLVDELKTWCMLKIQNLEQKLSGDSRACR
  AKSTPSTCESSTGVDQLVVTAGPAAASDSSPPVVRPKEKALNSTATQRLQQELSSSDCTGSRLRNVKVQT
  ALLPMNEAARSDQQAGPCVNRGTQTKKSGKSGPTRHRAQQPAASSTCGQPPPATGSEQTGPHIRDTSQAL
  ELTQYFFEAVSTQMEKWYERKIEEARSQANQKAQQDKATLKEHIKSLEEELAKLRTRVQKEN
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001242813
• ORF Size: 2076 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001242813.1, NP_001229742.1
• RefSeq Size: 5250 bp
• RefSeq ORF: 2079 bp
• Locus ID: 22881
• UniProt ID: Q9Y2G4
• Cytogenetics: 6q15
• Gene Summary: Recruits CKI-epsilon to the beta-catenin degradation complex that consists of AXN1 or AXN2 and GSK3-beta and allows efficient phosphorylation of beta-catenin, thereby inhibiting beta-catenin/Tcf signals.[UniProtKB/Swiss-Prot Function]