Hsp75 (TRAP1) (NM_001272049) Human Tagged ORF Clone
CAT#: RG234658
- TrueORF®
TRAP1 (tGFP-tagged) - Homo sapiens TNF receptor-associated protein 1 (TRAP1), transcript variant 2
ORF Plasmid: DDK
"NM_001272049" in other vectors (2)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Hsp75 |
Synonyms | HSP 75; HSP75; HSP90L; TRAP-1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG234658 representing NM_001272049
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCGCGAGCTGCGGGCGCTGCTGCTGTGGGGCCGCCGCCTGCGGCCTTTGCTGCGGGCGCCGGCGC TGGCGGCCGTGCCGGGAGGTTCCACTTCCAAACATGAGTTCCAGGCCGAGACAAAGAAGCTTTTGGACAT TGTTGCCCGGTCCCTGTACTCAGAAAAAGAGGTGTTTATACGGGAGCTGATCTCCAATGCCAGCGATGCC TTGGAAAAACTGCGTCACAAACTGGTGTCTGACGGCCAAGCACTGCCAGAAATGGAGATTCACTTGCAGA CCAATGCCGAGAAAGGCACCATCACCATCCAGGATACTGGTATCGGGATGACACAGGAAGAGCTGGTGTC CAACCTGGGGACGATTGCCAGATCGGGGTCAAAGGCCTTCCTGGATGCTCTGCAGAACCAGGCTGAGGCC AGCAGCAAGATCATCGGCCAGTTTGGAGTGGGTTTCTACTCAGCTTTCATGGTGGCTGACAGAGTGGAGG TCTATTCCCGCTCGGCAGCCCCGGGGAGCCTGGGTTACCAGTGGCTTTCAGATGGTTCTGGAGTGTTTGA AATCGCCGAAGCTTCGGGAGTTAGAACCGGGACAAAAATCATCATCCACCTGAAATCCGACTGCAAGGAG TTTTCCAGCGAGGCCCGGGTGCGAGATGTGGTAACGAAGTACAGCAACTTCGTCAGCTTCCCCTTGTACT TGAATGGAAGGCGGATGAACACCTTGCAGGCCATCTGGATGATGGACCCCAAGGATGTCCGTGAGTGGCA ACATGAGGAGTTCTACCGCTACGTCGCGCAGGCTCACGACAAGCCCCGCTACACCCTGCACTATAAGACG GACGCACCGCTCAACATCCGCAGCATCTTCTACGTGCCCGACATGAAACCGTCCATGTTTGATGTGAGCC GGGAGCTGGGCTCCAGCGTTGCACTGTACAGCCGCAAAGTCCTCATCCAGACCAAGGCCACGGACATCCT GCCCAAGTGGCTGCGCTTCATCCGAGGTGTGGTGGACAGTGAGGACATTCCCCTGAACCTCAGCCGGGAG CTGCTGCAGGAGAGCGCACTCATCAGGAAACTCCGGGACGTTTTACAGCAGAGGCTGATCAAATTCTTCA TTGACCAGAGTAAAAAAGATGCTGAGAAGTATGCAAAGTTTTTTGAAGATTACGGCCTGTTCATGCGGGA GGGCATTGTGACCGCCACCGAGCAGGAGGTCAAGGAGGACATAGCAAAGCTGCTGCGCTACGAGTCCTCG GCGCTGCCCTCCGGGCAGCTAACCAGCCTCTCAGAATACGCCAGCCGCATGCGGGCCGGCACCCGCAACA TCTACTACCTGTGCGCCCCCAACCGTCACCTGGCAGAGCACTCACCCTACTATGAGGCCATGAAGAAGAA AGACACAGAGGTTCTCTTCTGCTTTGAGCAGTTTGATGAGCTCACCCTGCTGCACCTTCGTGAGTTTGAC AAGAAGAAGCTGATCTCTGTGGAGACGGACATAGTCGTGGATCACTACAAGGAGGAGAAGTTTGAGGACA GGTCCCCAGCCGCCGAGTGCCTATCAGAGAAGGAGACGGAGGAGCTCATGGCCTGGATGAGAAATGTGCT GGGGTCGCGTGTCACCAACGTGAAGGTGACCCTCCGACTGGACACCCACCCTGCCATGGTCACCGTGCTG GAGATGGGGGCTGCCCGCCACTTCCTGCGCATGCAGCAGCTGGCCAAGACCCAGGAGGAGCGCGCACAGC TCCTGCAGCCCACGCTGGAGATCAACCCCAGGCACGCGCTCATCAAGAAGCTGAATCAGCTGCGCGCAAG CGAGCCTGGCCTGGCTCAGCTGCTGGTGGATCAGATATACGAGAACGCCATGATTGCTGCTGGACTTGTT GACGACCCTAGGGCCATGGTGGGCCGCTTGAATGAGCTGCTTGTCAAGGCCCTGGAGCGACAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG234658 representing NM_001272049
Red=Cloning site Green=Tags(s) MARELRALLLWGRRLRPLLRAPALAAVPGGSTSKHEFQAETKKLLDIVARSLYSEKEVFIRELISNASDA LEKLRHKLVSDGQALPEMEIHLQTNAEKGTITIQDTGIGMTQEELVSNLGTIARSGSKAFLDALQNQAEA SSKIIGQFGVGFYSAFMVADRVEVYSRSAAPGSLGYQWLSDGSGVFEIAEASGVRTGTKIIIHLKSDCKE FSSEARVRDVVTKYSNFVSFPLYLNGRRMNTLQAIWMMDPKDVREWQHEEFYRYVAQAHDKPRYTLHYKT DAPLNIRSIFYVPDMKPSMFDVSRELGSSVALYSRKVLIQTKATDILPKWLRFIRGVVDSEDIPLNLSRE LLQESALIRKLRDVLQQRLIKFFIDQSKKDAEKYAKFFEDYGLFMREGIVTATEQEVKEDIAKLLRYESS ALPSGQLTSLSEYASRMRAGTRNIYYLCAPNRHLAEHSPYYEAMKKKDTEVLFCFEQFDELTLLHLREFD KKKLISVETDIVVDHYKEEKFEDRSPAAECLSEKETEELMAWMRNVLGSRVTNVKVTLRLDTHPAMVTVL EMGAARHFLRMQQLAKTQEERAQLLQPTLEINPRHALIKKLNQLRASEPGLAQLLVDQIYENAMIAAGLV DDPRAMVGRLNELLVKALERH TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001272049 |
ORF Size | 1953 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001272049.2 |
RefSeq Size | 2153 bp |
RefSeq ORF | 1956 bp |
Locus ID | 10131 |
UniProt ID | Q12931 |
Cytogenetics | 16p13.3 |
Protein Families | Druggable Genome |
Gene Summary | This gene encodes a mitochondrial chaperone protein that is member of the heat shock protein 90 (HSP90) family. The encoded protein has ATPase activity and interacts with tumor necrosis factor type I. This protein may function in regulating cellular stress responses. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013] |
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