ABIN3 (TNIP3) (NM_001244764) Human Tagged ORF Clone

CAT#: RG233916

TNIP3 (tGFP-tagged) - Homo sapiens TNFAIP3 interacting protein 3 (TNIP3), transcript variant 3

Product Images

Circular map for RG233916

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ABIN3
• Synonyms: ABIN-3; LIND
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG233916 representing NM_001244764
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTCCATGTGGATGGCTCATGAACAAAAATGAGAAACACGACGATAAACTGGTAATGTTCACCAATC
  AATCTGAAGATTCTGAAAGATGTGAAAGCATGGAGCTGGACAAAAAAATCCAGGATCTGATTGAGAGAAA
  TGCGTCTCCTCATCCAAAACGGTTCACCCCAGAGGCCATGCCAACTCACAGAAATCTATGTTCTCTGAAG
  ACTCCAGGAAAAACAGCTTCCATGGCACATTTTGTACAGGGCACATCTAGAATGATTGCCGCAGAAAGTT
  CTACGGAGCATAAAGAGTGTGCTGAACCATCAACAAGAAAGAACTTGATGAATTCTCTTGAACAAAAGAT
  AAGGTGTTTGGAAAAACAAAGAAAAGAGCTCCTGGAAGTTAACCAGCAATGGGATCAGCAATTTAGAAGT
  ATGAAAGAGTTATATGAAAGAAAGGTAGCAGAGCTGAAGACGAAACTGGACGCCGCGGAAAGATTCCTCA
  GCACGCGGGAGAAGGATCCGCATCAGAGGCAGAGAAAGGACGACAGGCAGAGAGAGGACGACAGGCAGCG
  CGACCTGACCCGGGACCGGCTGCAGCGGGAGGAGAAGGAAAAGGAACGCCTAAATGAAGAATTACATGAA
  TTGAAAGAAGAGAATAAACTTTTAAAGGGAAAAAATACTCTTGCGAACAAGGAAAAGGAACATTACGAAT
  GTGAAATAAAACGCCTCAATAAGGCTCTTCAGGATGCCTTGAATATCAAGTGTTCATTTTCCGAGGACTG
  TTTGAGGAAGTCTCGAGTGGAATTCTGCCATGAGGAGATGAGAACAGAAATGGAAGTTCTGAAGCAGCAG
  GTGCAAATATACGAAGAAGACTTCAAAAAGGAACGATCGGATCGAGAGAGACTTAATCAAGAGAAAGAGG
  AGCTACAGCAAATTAATGAAACTTCCCAATCCCAGTTGAACAGGCTGAATTCCCAGATAAAAGCTTGTCA
  GATGGAGAAAGAAAAACTAGAAAAGCAATTAAAACAGATGTATTGCCCACCCTGTAACTGCGGCTTGGTT
  TTCCACCTGCAAGATCCATGGGTACCAACAGGCCCTGGAGCTGTGCAGAAGCAACGGGAGCACCCAGTTT
  ATCCTCAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG233916 representing NM_001244764
  Red=Cloning site Green=Tags(s)
  
  MIPCGWLMNKNEKHDDKLVMFTNQSEDSERCESMELDKKIQDLIERNASPHPKRFTPEAMPTHRNLCSLK
  TPGKTASMAHFVQGTSRMIAAESSTEHKECAEPSTRKNLMNSLEQKIRCLEKQRKELLEVNQQWDQQFRS
  MKELYERKVAELKTKLDAAERFLSTREKDPHQRQRKDDRQREDDRQRDLTRDRLQREEKEKERLNEELHE
  LKEENKLLKGKNTLANKEKEHYECEIKRLNKALQDALNIKCSFSEDCLRKSRVEFCHEEMRTEMEVLKQQ
  VQIYEEDFKKERSDRERLNQEKEELQQINETSQSQLNRLNSQIKACQMEKEKLEKQLKQMYCPPCNCGLV
  FHLQDPWVPTGPGAVQKQREHPVYPQ
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001244764
• ORF Size: 1128 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001244764.2
• RefSeq Size: 2450 bp
• RefSeq ORF: 1131 bp
• Locus ID: 79931
• UniProt ID: Q96KP6
• Cytogenetics: 4q27
• Gene Summary: Binds to zinc finger protein TNFAIP3 and inhibits NF-kappa-B activation induced by tumor necrosis factor, Toll-like receptor 4 (TLR4), interleukin-1 and 12-O-tetradecanoylphorbol-13-acetate. Overexpression inhibits NF-kappa-B-dependent gene expression in response to lipopolysaccharide at a level downstream of TRAF6 and upstream of IKBKB. NF-kappa-B inhibition is independent of TNFAIP3 binding.[UniProtKB/Swiss-Prot Function]