RTBDN (NM_001270442) Human Tagged ORF Clone

CAT#: RG233509

RTBDN (tGFP-tagged) - Homo sapiens retbindin (RTBDN), transcript variant 5

Product Images

Circular map for RG233509

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: RTBDN
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG233509 representing NM_001270442
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGGCAGGTGGACATGGACTGCAGGGTCCACATGCGACCCATCGGCCTGACGTGGGTGCTGCAAC
  TGACCTTGGCATGGATCCTGCTAGAAGCCTGTGGAGGGAGCCGCCCACTCCAAGCCAGGTCCCAGCAACA
  CCATGGGCTGGCAGCTGATCTGGGCAAAGGCAAGCTGCACCTGGCAGGACCTTGTTGTCCCTCAGAGATG
  GACACAACAGAGACATCGGGCCCTGGAAACCATCCAGAACGCTGTGGAGTGCCGAGCCCTGAATGCGAAT
  CCTTCCTGGAACACCTCCAACGTGCCCTTCGCAGTCGCTTCCGCCTGCGGCTATTGGGGGTACGCCAGGC
  ACAGCCGCTCTGCGAGGAGCTCTGCCAGGCCTGGTTCGCCAACTGCGAAGATGATATCACCTGCGGCCCG
  ACTTGGCTCCCACTCTCAGAAAAAAGGGGCTGTGAGCCCAGCTGCCTTACCTATGGACAGACCTTCGCAG
  ACGGGACGGACCTTTGTCGCTCGGCTCTGGGCCACGCCCTACCGGTGGCTGCTCCTGGAGCCCGTCACTG
  CTTCAACATCTCCATCTCCGCGGTACCTCGTCCCAGACCAGGACGACGGGGCCGGGAAGCTCCCTCCCGG
  CGTTCCCGCAGCCCTCGCACCTCCATCCTGGACGCTGCGGGCAGCGGGAGTGGCAGTGGAAGCGGCAGCG
  GCCCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG233509 representing NM_001270442
  Red=Cloning site Green=Tags(s)
  
  MARQVDMDCRVHMRPIGLTWVLQLTLAWILLEACGGSRPLQARSQQHHGLAADLGKGKLHLAGPCCPSEM
  DTTETSGPGNHPERCGVPSPECESFLEHLQRALRSRFRLRLLGVRQAQPLCEELCQAWFANCEDDITCGP
  TWLPLSEKRGCEPSCLTYGQTFADGTDLCRSALGHALPVAAPGARHCFNISISAVPRPRPGRRGREAPSR
  RSRSPRTSILDAAGSGSGSGSGSGP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001270442
• ORF Size: 705 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001270442.1, NP_001257371.1
• RefSeq Size: 1030 bp
• RefSeq ORF: 708 bp
• Locus ID: 83546
• UniProt ID: Q9BSG5
• Cytogenetics: 19p13.13
• Protein Families: Druggable Genome, Secreted Protein
• Gene Summary: This gene was first identified in a study of human eye tissues. The protein encoded by this gene is preferentially expressed in the retina and may play a role in binding retinoids and other carotenoids as it shares homology with riboflavin binding proteins. Alternative splicing results in multiple transcript variants and protein isoforms. [provided by RefSeq, Jul 2012]