Hydroxysteroid (17 beta) Dehydrogenase 4 (HSD17B4) (NM_001199291) Human Tagged ORF Clone
CAT#: RG233127
- TrueORF®
HSD17B4 (tGFP-tagged) - Homo sapiens hydroxysteroid (17-beta) dehydrogenase 4 (HSD17B4), transcript variant 1
ORF Plasmid: DDK
"NM_001199291" in other vectors (2)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Hydroxysteroid (17 beta) Dehydrogenase 4 |
Synonyms | DBP; MFE-2; MFP-2; MPF-2; PRLTS1; SDR8C1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG233127 representing NM_001199291
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTTATTCTTGAGGCACCGCATCTCTTGAGGAGGAAAGAGCCGGAAACACCTGGTCTCTCAAGCAGGA TTGGGCCGAGCCTATGCCCTGGCTTTTGCAGAAAGAGGAGCGTTAGTTGTTGCTTTCAAAATCTATGCAA TAACCCTATGGAGAAGATCATTTCACAATGCAGATTCTTTGTTTCAATGAATGATTTGGGAGGGGACTTC AAAGGAGTTGGTAAAGGCTCCTTAGCTGCTGATAAGGTTGTTGAAGAAATAAGAAGGAGAGGTGGAAAAG CAGTGGCCAACTATGATTCAGTGGAAGAAGGAGAGAAGGTTGTGAAGACAGCCCTGGATGCTTTTGGAAG AATAGATGTTGTGGTCAACAATGCTGGAATTCTGAGGGATCGTTCCTTTGCTAGGATAAGTGATGAAGAC TGGGATATAATCCACAGAGTTCATTTGCGGGGTTCATTCCAAGTGACACGGGCAGCATGGGAACACATGA AGAAACAGAAGTATGGAAGGATTATTATGACTTCATCAGCTTCAGGAATATATGGCAACTTTGGCCAGGC CAATTATAGTGCTGCAAAGTTGGGTCTTCTGGGCCTTGCAAATTCTCTTGCAATTGAAGGCAGGAAAAGC AACATTCATTGTAACACCATTGCTCCTAATGCGGGATCACGGATGACTCAGACAGTTATGCCTGAAGATC TTGTGGAAGCCCTGAAGCCAGAGTATGTGGCACCTCTTGTCCTTTGGCTTTGTCACGAGAGTTGTGAGGA GAATGGTGGCTTGTTTGAGGTTGGAGCAGGATGGATTGGAAAATTACGCTGGGAGCGGACTCTTGGAGCT ATTGTAAGACAAAAGAATCACCCAATGACTCCTGAGGCAGTCAAGGCTAACTGGAAGAAGATCTGTGACT TTGAGAATGCCAGCAAGCCTCAGAGTATCCAAGAATCAACTGGCAGTATAATTGAAGTTCTGAGTAAAAT AGATTCAGAAGGAGGAGTTTCAGCAAATCATACTAGTCGTGCAACGTCTACAGCAACATCAGGATTTGCT GGAGCTATTGGCCAGAAACTCCCTCCATTTTCTTATGCTTATACGGAACTGGAAGCTATTATGTATGCCC TTGGAGTGGGAGCGTCAATCAAGGATCCAAAAGATTTGAAATTTATTTATGAAGGAAGTTCTGATTTCTC CTGTTTGCCCACCTTCGGAGTTATCATAGGTCAGAAATCTATGATGGGTGGAGGATTAGCAGAAATTCCT GGACTTTCAATCAACTTTGCAAAGGTTCTTCATGGAGAGCAGTACTTAGAGTTATATAAACCACTTCCCA GAGCAGGAAAATTAAAATGTGAAGCAGTTGTTGCTGATGTCCTAGATAAAGGATCCGGTGTAGTGATTAT TATGGATGTCTATTCTTATTCTGAGAAGGAACTTATATGCCACAATCAGTTCTCTCTCTTTCTTGTTGGC TCTGGAGGCTTTGGTGGAAAACGGACATCAGACAAAGTCAAGGTAGCTGTAGCCATACCTAATAGACCTC CTGATGCTGTACTTACAGATACCACCTCTCTTAATCAGGCTGCTTTGTACCGCCTCAGTGGAGACTGGAA TCCCTTACACATTGATCCTAACTTTGCTAGTCTAGCAGGTTTTGACAAGCCCATATTACATGGATTATGT ACATTTGGATTTTCTGCCAGGCGTGTGTTACAGCAGTTTGCAGATAATGATGTGTCAAGATTCAAGGCAA TTAAGGCTCGTTTTGCAAAACCAGTATATCCAGGACAAACTCTACAAACTGAGATGTGGAAGGAAGGAAA CAGAATTCATTTTCAAACCAAGGTCCAAGAAACTGGAGACATTGTCATTTCAAATGCATATGTGGATCTT GCACCAACATCTGGTACTTCAGCTAAGACACCCTCTGAGGGCGGGAAGCTTCAGAGTACCTTTGTATTTG AGGAAATAGGACGCCGCCTAAAGGATATTGGGCCTGAGGTGGTGAAGAAAGTAAATGCTGTATTTGAGTG GCATATAACCAAAGGCGGAAATATTGGGGCTAAGTGGACTATTGACCTGAAAAGTGGTTCTGGAAAAGTG TACCAAGGCCCTGCAAAAGGTGCTGCTGATACAACAATCATACTTTCAGATGAAGATTTCATGGAGGTGG TCCTGGGCAAGCTTGACCCTCAGAAGGCATTCTTTAGTGGCAGGCTGAAGGCCAGAGGGAACATCATGCT GAGCCAGAAACTTCAGATGATTCTTAAAGACTACGCCAAGCTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG233127 representing NM_001199291
Red=Cloning site Green=Tags(s) MVILEAPHLLRRKEPETPGLSSRIGPSLCPGFCRKRSVSCCFQNLCNNPMEKIISQCRFFVSMNDLGGDF KGVGKGSLAADKVVEEIRRRGGKAVANYDSVEEGEKVVKTALDAFGRIDVVVNNAGILRDRSFARISDED WDIIHRVHLRGSFQVTRAAWEHMKKQKYGRIIMTSSASGIYGNFGQANYSAAKLGLLGLANSLAIEGRKS NIHCNTIAPNAGSRMTQTVMPEDLVEALKPEYVAPLVLWLCHESCEENGGLFEVGAGWIGKLRWERTLGA IVRQKNHPMTPEAVKANWKKICDFENASKPQSIQESTGSIIEVLSKIDSEGGVSANHTSRATSTATSGFA GAIGQKLPPFSYAYTELEAIMYALGVGASIKDPKDLKFIYEGSSDFSCLPTFGVIIGQKSMMGGGLAEIP GLSINFAKVLHGEQYLELYKPLPRAGKLKCEAVVADVLDKGSGVVIIMDVYSYSEKELICHNQFSLFLVG SGGFGGKRTSDKVKVAVAIPNRPPDAVLTDTTSLNQAALYRLSGDWNPLHIDPNFASLAGFDKPILHGLC TFGFSARRVLQQFADNDVSRFKAIKARFAKPVYPGQTLQTEMWKEGNRIHFQTKVQETGDIVISNAYVDL APTSGTSAKTPSEGGKLQSTFVFEEIGRRLKDIGPEVVKKVNAVFEWHITKGGNIGAKWTIDLKSGSGKV YQGPAKGAADTTIILSDEDFMEVVLGKLDPQKAFFSGRLKARGNIMLSQKLQMILKDYAKL TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001199291 |
ORF Size | 2283 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001199291.3 |
RefSeq Size | 2899 bp |
RefSeq ORF | 2286 bp |
Locus ID | 3295 |
UniProt ID | P51659 |
Cytogenetics | 5q23.1 |
Protein Families | Druggable Genome |
Protein Pathways | Metabolic pathways, Primary bile acid biosynthesis |
Gene Summary | The protein encoded by this gene is a bifunctional enzyme that is involved in the peroxisomal beta-oxidation pathway for fatty acids. It also acts as a catalyst for the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-branched-chain fatty acids. Defects in this gene that affect the peroxisomal fatty acid beta-oxidation activity are a cause of D-bifunctional protein deficiency (DBPD). An apparent pseudogene of this gene is present on chromosome 8. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014] |
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