DCDC1 (NM_020869) Human Tagged ORF Clone
CAT#: RG231457
- TrueORF®
DCDC5 (tGFP-tagged) - Human doublecortin domain containing 5 (DCDC5)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_020869" in other vectors (3)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | DCDC1 |
Synonyms | DCDC5 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG231457 representing NM_020869
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGCCTGTCCTTCCCAGTGGCCAACTTAATGAGGAGTTTGATTGGCCAATACAAGGACTGCTTGTTC CGAGCAGTCCTCCCATGAAGAAACCCATCTGTAAGACAACAGAGCCATATGCCCCTGTGCGACTCAGAGT TTTGCAGAATGGAGAGAAGAATAAAAACAGATCCGTTACTATCCTTGGTCCAGATATCTCACCTGGACGG AAAACGCAGTGCACTGAGATTTTAAATTTACCTTCTGCAGCTCGGAGATTGTACAATGAAAAGGGGAAGG AAATATTTGCCTTAAAAGACCTGCAAAGAGATGAACTGGTGTATGTTTCATGTGGAGAACTCTGGATCAA TCCTGACCTGTCCATTGCTCAGCAAAAGAAACAAATATTCCTGAGGAACCTAGAATCAGACATTGCCAAA ATTCAAATCTTCTGCAGCACACATAAAATAGAAGCTCTTGTTTTAGAAGTCCAAAGTGACATTGTATCTG GAAGCAAGCTTGCTGTGCATAAACCTGTAGCAATTTTTGGAGAAGAGAAGCAAGTTACAGAACCGGAAGA AAAGCAAATGCAAGAAGATCCTCTAACAACGGAAAATGCTTCCAGTGAAATTCTAGATTCACACGTAAGA GCTCATCTTCGAATGAAGGCTTGTCACACACTTCCCAGGTATGCCTGGCAGGAAACTTCACATGACTTTG ATGAGGATGACAGTCTTCCAAAGAAAACGGAAAAAGGGCTCTTTGAAAATGTGGAACCACAGAAGAAACA CAGCTGTTCACCAAAGCATAGTAAATTGCACAAGCATTGTCATCAGCAGTTCGAATACAGAGATGGGCAG ATTATAAGCCATGCTGCTCCTCAGCTAGTCTTGGGGGTTCAAGGTCCCAATCTCCGATCAGGCATGGAGG TGGTTTTGGTGGAGAAGAAATCTGATGGTAGTCATCAGCGCTGGATACACCAGGAAGACAGCAGGACCTT TCACCTTGTGAGTAACCCTGACCTTGTGCTGGCAGTGTCTATGACCAAGACTAGAAATGAAGTTTGTGGC TATCCAGTTATTGTTCAGAAATATAAGCCGTACAACAATGGAGCTGCCAATCAAAAGTGGCATTACATGA AAAATATAAAAGCACTTGTGGCCTTTCATAGCACTGCCTTGGATAAGGAAATTACATCAGCAAATTATGC TGGTGTCTGTACATCATCTGTGATTAAAGAAGAAAACATTGATCAACCAGGATACTGTTATCTCTCACCT GATGGAAAGAGAAAAACTATGCTCTGCTTGGCTTGTGGACAATCCATGAGAACAGAGAAAGGACTGAAAC AATTGCTTCCAGGGGTTCCATTCCTCTGTATTTCAGGCACCAAGACTCAGAAGCCCTTCTTACAAGGGCC CTTCAAGGTCATCAGTGTGGCTGAGGTTGATTTGTCGTGTGACAAGGCTGAAAAAACTCTAAGTTACTAC CAAGCACGTCTATTGTCTTTACGGATGAAGACCTGCACGCAAGCTGCATCTCACAGTGGCATGGCAGCCA CACACCAGAAGGCAGTGAAAATAATTGCATACAAAAATGGGGATGGGTATCGTAATGGGAAGTTAATTGT GGCTGGAACATTCCCCATGCTTCTTACAGAATGCACGGAACAACTTGGGCTTGCCAGAGCAGCCTCCAAA GTATATACCAAAGATGGAACCCCAATCTTTACCTTGCGTGATTTGGTTTTATGGGCTCTAGATGAATCCT TTCTCCAGAGAGACTCTGAGAAACAAAAGCAAGATGCAGCTCCTGTTGGAAAAGAACAGATAATTGTTGA AAAAAATCCAAGAATGAAAGTGAAAAACAGATTATTTGCAAAATCTGTGACATCCGATAGTTTGGATGGT ATAGACAAGTCTTTGCTTACCCTCATCCTCAGAAATCCTATTGCCATCTGGGTGTCTTGTGGTGAACCAT TTCTACCTCCAAATGCTTTGCAGAAAGCAGAAAAATTAGAGAAACAGAACTGGCTAAAAAAGGACAGAAT TTTGGCTGATCTAGATACCATGAGACACAAAATGAGACAGTTAAAAGGGCGGCGAGTAGCGGCATGTCAG CCAGCCACCATGGTTCCTACCAAGAGCCCTGTGCAGCCCGTGGTGGTTGAAGGAGGCTGGACCGAACAGA CTCAACAGGAAATTAAACTCATGGAACTTATAAGACATACAGAGGCACACCTTTCTGAAATCCAAGAAAT GGAATCCAAAATAAATTTTCCAATTGCAACCAAACGTATAGCAGTCAAGCCGAGCAACCTGTATAAGCAG CCCAACACAAAACGAGTGTGGATTTATCTAAATGGAGGCAGACCTGAAGATGGCACTTATGCCTGGGGCA AAACTATTTCAGAGCTGCTGCAAGACTGCTCCTCTCGTCTCAAAATGACCCACCCAGCTAGAGCACTGTA CACCCCCAGTGGAGAGCCAATTCAGTCCTGGGACGACATAGAGCGAGATATGGTCATCTGTGTGTCTATG GGACATGGTTTCAAAACCCCAAAAGAGTTAAAACAACTGATGGAGATCAGAGCAAATTATGCCAGAATCC GAAGGCAGCAGGGCCCTCAAGCCACAGACATTGTGGTGTCACCATCCACGAAGCTGCTGTCTCTGGCACA TCTCCACAAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG231457 representing NM_020869
Red=Cloning site Green=Tags(s) MWPVLPSGQLNEEFDWPIQGLLVPSSPPMKKPICKTTEPYAPVRLRVLQNGEKNKNRSVTILGPDISPGR KTQCTEILNLPSAARRLYNEKGKEIFALKDLQRDELVYVSCGELWINPDLSIAQQKKQIFLRNLESDIAK IQIFCSTHKIEALVLEVQSDIVSGSKLAVHKPVAIFGEEKQVTEPEEKQMQEDPLTTENASSEILDSHVR AHLRMKACHTLPRYAWQETSHDFDEDDSLPKKTEKGLFENVEPQKKHSCSPKHSKLHKHCHQQFEYRDGQ IISHAAPQLVLGVQGPNLRSGMEVVLVEKKSDGSHQRWIHQEDSRTFHLVSNPDLVLAVSMTKTRNEVCG YPVIVQKYKPYNNGAANQKWHYMKNIKALVAFHSTALDKEITSANYAGVCTSSVIKEENIDQPGYCYLSP DGKRKTMLCLACGQSMRTEKGLKQLLPGVPFLCISGTKTQKPFLQGPFKVISVAEVDLSCDKAEKTLSYY QARLLSLRMKTCTQAASHSGMAATHQKAVKIIAYKNGDGYRNGKLIVAGTFPMLLTECTEQLGLARAASK VYTKDGTPIFTLRDLVLWALDESFLQRDSEKQKQDAAPVGKEQIIVEKNPRMKVKNRLFAKSVTSDSLDG IDKSLLTLILRNPIAIWVSCGEPFLPPNALQKAEKLEKQNWLKKDRILADLDTMRHKMRQLKGRRVAACQ PATMVPTKSPVQPVVVEGGWTEQTQQEIKLMELIRHTEAHLSEIQEMESKINFPIATKRIAVKPSNLYKQ PNTKRVWIYLNGGRPEDGTYAWGKTISELLQDCSSRLKMTHPARALYTPSGEPIQSWDDIERDMVICVSM GHGFKTPKELKQLMEIRANYARIRRQQGPQATDIVVSPSTKLLSLAHLHN TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_020869 |
ORF Size | 2670 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_020869.3, NP_065920.2 |
RefSeq Size | 4773 bp |
RefSeq ORF | 2673 bp |
Locus ID | 341019 |
Cytogenetics | 11p13 |
Gene Summary | This gene encodes a member of the doublecortin family. The protein encoded by this gene is a hydrophilic, intracellular protein. It contains a single doublecortin domain and is unable to bind microtubules and to regulate microtubule polymerization. This gene is mainly expressed in adult testis. It does not have a mouse homolog. [provided by RefSeq, Sep 2010] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC231457 | DCDC5 (Myc-DDK-tagged)-Human doublecortin domain containing 5 (DCDC5) |
USD 815.00 |
|
RC231457L3 | Lenti-ORF clone of DCDC5 (Myc-DDK-tagged)-Human doublecortin domain containing 5 (DCDC5) |
USD 1,115.00 |
|
RC231457L4 | Lenti-ORF clone of DCDC5 (mGFP-tagged)-Human doublecortin domain containing 5 (DCDC5) |
USD 1,115.00 |
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