NT5C1B (NT5C1B-RDH14) (NM_001199104) Human Tagged ORF Clone

CAT#: RG231332

NT5C1B (tGFP-tagged) - Human NT5C1B-RDH14 readthrough (NT5C1B-RDH14), transcript variant 2

Product Images

Circular map for RG231332

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: NT5C1B
• Synonyms: AIRP; cN-IB; cN1B; NT5C1B
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG231332 representing NM_001199104
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTCAAACATCTCTCAAACAGAAAAAGAATGAGCCTGGAATGAGGTCCTCAAAAGAGAGTCTAGAAG
  CAGAAAAAAGAAAGGAATCTGACAAAACAGGAGTTCGTCTGAGCAATCAGATGAGGCGTGCAGTCAATCC
  GAATCACTCGCTGAGATGTTGCCCCTTCCAGGGTCACTCGTCGTGTAGACGCTGCCTTTGTGCAGCTGAG
  GGAACAGCCCTTGGCCCCTGCCACACAATACGTATTTATATTCACATGTGCCTGTTGTGGGAGCAGGGCC
  AGCAGATCACCATGATGAGGGGATCACAAGAATCATCACTGCGGAAGACAGACTCTCGAGGGTACCTTGT
  GCGCAGTCAATGGTCTAGAATATCCCGGAGCCCATCCACCAAGGCTCCATCCATAGATGAGCCTAGAAGC
  AGGAACACCAGTGCTAAGCTCCCCAGCAGCTCCACGAGCTCCCGGACTCCATCCACCTCCCCAAGCCTGC
  ATGACTCCTCACCGCCGCCGCTGTCCGGGCAGCCCTCGCTCCAGCCACCCGCGTCGCCCCAGCTGCCCCG
  GTCGCTGGACTCGCGGCCTCCCACGCCCCCAGAGCCCGATCCTGGCTCCCGGCGCAGCACCAAAATGCAA
  GAGAATCCGGAGGCCTGGGCCCAAGGCATCGTGCGGGAAATCCGCCAGACCCGGGACTCGCAGCCGCTGG
  AATATTCGCGCACGTCCCCCACCGAGTGGAAGTCCTCCAGCCAGCGCAGGGGGATCTACCCCGCCTCCAC
  CCAGCTGGACCGCAACTCTCTGTCCGAGCAGCAGCAGCAGCAGCGGGAGGACGAGGACGACTACGAGGCT
  GCCTACTGGGCATCCATGAGGTCGTTCTACGAGAAGAACCCGAGCTGCTCGCGCCCCTGGCCGCCCAAAC
  CCAAGAACGCCATCACCATTGCTCTCTCATCCTGCGCGCTCTTCAACATGGTGGACGGCAGGAAAATCTA
  CGAGCAAGAGGGTCTGGAAAAGTACATGGAGTATCAGCTCACCAATGAGAACGTCATCCTGACCCCGGGC
  CCGGCGTTCCGCTTCGTCAAGGCACTACAGTATGTCAATGCTAGACTCCGTGATCTATATCCTGATGAAC
  AGGACTTATTTGATATTGTACTGATGACTAATAACCATGCCCAAGTGGGAGTGCGGCTTATAAACAGCGT
  CAATCACTACGGCTTACTGATTGACCGCTTCTGTCTGACCGGGGGAAAAGACCCCATTGGCTATTTGAAG
  GCATATCTTACCAACTTGTATATTGCTGCAGATTCTGAAAAAGTGCAAGAGGCAATACAAGAAGGTATTG
  CCTCTGCGACAATGTTTGATGGAGCCAAAGACATGGCTTACTGTGACACTCAGCTCCGTGTAGCCTTTGA
  TGGGGATGCTGTCCTCTTCTCTGATGAGTCTGAACATTTTACCAAGGAGCATGGGCTCGACAAATTCTTC
  CAGTATGATACATTATGTGAAAGTAAGCCTCTTGCTCAGGGTCCCCTAAAAGGCTTTCTGGAAGATTTAG
  GCAGACTGCAAAAGAAGTTCTATGCCAAAAATGAACGGTTACTTTGTCCTATCAGGACCTACCTGGTTAC
  AGCTAGGAGTGCAGCCAGTTCAGGCGCCCGTGTGCTGAAGACCCTTCGACGCTGGGGTCTAGAGATAGAC
  GAAGCTCTTTTCCTTGCTGGAGCCCCCAAAAGTCCCATCTTGGTGAAGATCCGGCCCCACATCTTCTTTG
  ATGACCACATGTTCCACATTGAAGGGGCACAGAGGAAGAGCCTAGGCTGGATGTCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG231332 representing NM_001199104
  Red=Cloning site Green=Tags(s)
  
  MSQTSLKQKKNEPGMRSSKESLEAEKRKESDKTGVRLSNQMRRAVNPNHSLRCCPFQGHSSCRRCLCAAE
  GTALGPCHTIRIYIHMCLLWEQGQQITMMRGSQESSLRKTDSRGYLVRSQWSRISRSPSTKAPSIDEPRS
  RNTSAKLPSSSTSSRTPSTSPSLHDSSPPPLSGQPSLQPPASPQLPRSLDSRPPTPPEPDPGSRRSTKMQ
  ENPEAWAQGIVREIRQTRDSQPLEYSRTSPTEWKSSSQRRGIYPASTQLDRNSLSEQQQQQREDEDDYEA
  AYWASMRSFYEKNPSCSRPWPPKPKNAITIALSSCALFNMVDGRKIYEQEGLEKYMEYQLTNENVILTPG
  PAFRFVKALQYVNARLRDLYPDEQDLFDIVLMTNNHAQVGVRLINSVNHYGLLIDRFCLTGGKDPIGYLK
  AYLTNLYIAADSEKVQEAIQEGIASATMFDGAKDMAYCDTQLRVAFDGDAVLFSDESEHFTKEHGLDKFF
  QYDTLCESKPLAQGPLKGFLEDLGRLQKKFYAKNERLLCPIRTYLVTARSAASSGARVLKTLRRWGLEID
  EALFLAGAPKSPILVKIRPHIFFDDHMFHIEGAQRKSLGWMS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001199104
• ORF Size: 1806 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001199104.1, NP_001186033.1
• RefSeq Size: 2982 bp
• RefSeq ORF: 1809 bp
• Locus ID: 100526794
• UniProt ID: Q96P26
• Cytogenetics: 2p24.2
• Gene Summary: This locus represents naturally occurring read-through transcription between the neighboring NT5C1B (5'-nucleotidase, cytosolic IB) and RDH14 (retinol dehydrogenase 14) genes on chromosome 2. Alternative splicing results in multiple transcript variants, one of which encodes a fusion protein that shares sequence identity with the products of each individual gene. [provided by RefSeq, Nov 2010]