MCF2 (NM_001171876) Human Tagged ORF Clone

CAT#: RG230610

MCF2 (tGFP-tagged) - Human MCF.2 cell line derived transforming sequence (MCF2), transcript variant 3

Product Images

Circular map for RG230610

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MCF2
• Synonyms: ARHGEF21; DBL
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG230610 representing NM_001171876
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAAGACATCGCCTTCTTGTCTGGTGGCCGGGGAAAGGACAATGCTTGGATCATTACGTTTCCAGAAA
  ACTGTAATTTTAGATGTATACCAGAGGAAGTAATAGCAAAAGTACTTACTTACCTGACATCTATTGCAAG
  GCAAAATGGATCAGACTCCCGGTTTACCATTATTCTGGATCGAAGATTGGATACATGGTCTTCTCTCAAA
  ATCTCTCTCCAAAAAATCTCGGCTTCCTTCCCTGGGAACTTGCACTTGGTTTTGGTTTTACGTCCTACCA
  GCTTTCTTCAACGAACGTTCACAGACATTGGATTTTGGTTTAGTCAGGAGGATTTTATGCTTAAATTACC
  AGTTGTTATGCTGAGCTCAGTTAGTGATTTGCTGACATACATTGATGACAAGCAATTAACCCCTGAGTTA
  GGCGGCACCTTGCAGTACTGCCACAGTGAATGGATCATCTTCAGAAATGCTATAGAAAATTTTGCCCTCA
  CAGTGAAAGAAATGGCTCAGATGTTACAGTCCTTTGGAACTGAACTGGCTGAGACAGAACTACCAGATGA
  TATTCCCTCAATAGAAGAAATTCTGGCAATTCGTGCTGAAAGGTATCATCTGTTGAAGAATGATATTACA
  GCTGTAACCAAAGAAGGAAAAATTCTGCTAACAAATCTGGAAGTGCCTGACACTGAAGGAGCTGTCAGTT
  CAAGACTAGAATGTCATCGGCAAATAAGTGGTGACTGGCAAACTATTAATAAGTTGCTGACTCAAGTACA
  TGATATGGAAACAGCTTTTGATGGATTTTGGGAAAAACATCAATTAAAAATGGAGCAGTATCTGCAACTA
  TGGAAGTTTGAGCAGGATTTTCAACAGCTTGTGACTGAAGTTGAATTTCTATTAAACCAACAAGCAGAAC
  TGGCTGATGTAACAGGGACTATAGCTCAAGTAAAACAAAAAATAAAAAAATTGGAAAACTTAGATGAAAA
  TTCTCAGGAGCTATTATCAAAGGCCCAGTTTGTGATATTACATGGACACAAGCTTGCAGCAAATCACCAT
  TATGCACTTGATTTAATCTGCCAGAGGTGCAATGAGCTACGTTACCTTTCTGATATTTTGGTTAATGAGA
  TAAAAGCAAAACGGATACAACTCAGCAGGACCTTCAAAATGCATAAACTCCTACAGCAGGCTCGTCAATG
  CTGTGATGAAGGGGAATGTCTTCTAGCTAATCAGGAAATAGATAAGTTTCAGTCTAAAGAAGATGCTCAG
  AAAGCTCTCCAAGACATTGAAAATTTTCTTGAAATGGCTCTACCCTTTATAAATTATGAACCTGAAACAC
  TGCAGTATGAATTTGATGTAATATTATCTCCTGAGCTTAAGGTTCAAATGAAGACTATACAACTCAAGCT
  TGAAAACATTCGAAGTATATTTGAGAACCAGCAGGCTGGTTTCAGGAACCTGGCAGATAAGCATGTGAGG
  CCAATCCAATTTGTGGTACCCACACCTGAAAATTTGGTCACATCTGGGACACCATTTTTTTCATCTAAAC
  AAGTGGGAGTTGGATACTCTTTCTTTCAAGCATGTAAACTTTTTTCAAAAGGGAAGAAGACTTGGAGACA
  AAATCAGAGCAACTTAAAAATTGAAGTGGTGCCTGATTGTCAGGAGAAGAGAAGTTCTGGTCCATCCTCC
  AGTTTGGACAATGGCAATAGCTTGGATGTTTTAAAGAACCACGTACTAAATGAACTGATACAGACTGAGA
  GAGTTTATGTTCGAGAACTGTATACTGTTTTGTTGGGTTATAGAGCGGAGATGGATAATCCAGAGATGTT
  TGATCTTATGCCACCTCTCCTGAGAAATAAAAAGGACATTCTCTTTGGAAACATGGCAGAAATATATGAA
  TTCCATAACGACATTTTCTTGAGCAGCCTGGAAAATTGTGCTCATGCTCCAGAAAGAGTGGGACCTTGTT
  TCCTGGAAAGGAAGGATGATTTTCAGATGTATGCAAAATATTGTCAGAATAAGCCCAGATCAGAAACAAT
  TTGGAGGAAGTATTCAGAATGCGCATTTTTCCAGGAATGTCAAAGAAAGTTAAAACACAGACTTAGACTG
  GATTCCTATTTACTCAAACCAGTGCAACGAATCACTAAATATCAGTTATTGTTGAAGGAGCTATTAAAAT
  ATAGCAAAGACTGTGAAGGATCTGCTCTGTTGAAGAAGGCACTCGATGCAATGCTGGATTTACTGAAGTC
  AGTTAATGATTCTATGCATCAGATTGCAATAAATGGCTATATTGGAAACTTAAATGAACTGGGCAAGATG
  ATAATGCAAGGTGGATTCAGCGTTTGGATAGGGCACAAGAAAGGTGCTACAAAAATGAAGGATTTGGCTA
  GATTCAAACCAATGCAGCGACACCTTTTCTTGTATGAAAAAGCCATTGTTTTTTGCAAAAGGCGTGTTGA
  AAGTGGAGAAGGCTCTGACAGATACCCGTCATACAGTTTTAAACACTGTTGGAAAATGGATGAAGTTGGA
  ATCACTGAATATGTAAAAGGTGATAACCGCAAGTTTGAAATCTGGTATGGTGAAAAGGAAGAAGTTTATA
  TTGTCCAGGCTTCTAATGTAGATGTGAAGATGACGTGGCTAAAAGAAATAAGAAATATTTTGTTGAAGCA
  GCAGGAACTTTTGACAGTTAAAAAAAGAAAGCAACAGGATCAATTAACAGAACGGGATAAGTTTCAGATT
  TCTCTTCAGCAGAATGATGAAAAGCAACAGGGAGCTTTTATAAGTACTGAGGAAACTGAATTGGAACACA
  CCAGCACTGTGGTGGAGGTCTGTGAGGCAATTGCGTCAGTTCAGGCAGAAGCAAATACAGTTTGGACTGA
  GGCATCACAATCTGCAGAAATCTCTGAAGAACCTGCGGAATGGTCAAGCAACTATTTCTACCCTACTTAT
  GATGAAAATGAAGAAGAAAATAGGCCCCTCATGAGACCTGTGTCGGAGATGGCTCTCCTATAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG230610 representing NM_001171876
  Red=Cloning site Green=Tags(s)
  
  MQDIAFLSGGRGKDNAWIITFPENCNFRCIPEEVIAKVLTYLTSIARQNGSDSRFTIILDRRLDTWSSLK
  ISLQKISASFPGNLHLVLVLRPTSFLQRTFTDIGFWFSQEDFMLKLPVVMLSSVSDLLTYIDDKQLTPEL
  GGTLQYCHSEWIIFRNAIENFALTVKEMAQMLQSFGTELAETELPDDIPSIEEILAIRAERYHLLKNDIT
  AVTKEGKILLTNLEVPDTEGAVSSRLECHRQISGDWQTINKLLTQVHDMETAFDGFWEKHQLKMEQYLQL
  WKFEQDFQQLVTEVEFLLNQQAELADVTGTIAQVKQKIKKLENLDENSQELLSKAQFVILHGHKLAANHH
  YALDLICQRCNELRYLSDILVNEIKAKRIQLSRTFKMHKLLQQARQCCDEGECLLANQEIDKFQSKEDAQ
  KALQDIENFLEMALPFINYEPETLQYEFDVILSPELKVQMKTIQLKLENIRSIFENQQAGFRNLADKHVR
  PIQFVVPTPENLVTSGTPFFSSKQVGVGYSFFQACKLFSKGKKTWRQNQSNLKIEVVPDCQEKRSSGPSS
  SLDNGNSLDVLKNHVLNELIQTERVYVRELYTVLLGYRAEMDNPEMFDLMPPLLRNKKDILFGNMAEIYE
  FHNDIFLSSLENCAHAPERVGPCFLERKDDFQMYAKYCQNKPRSETIWRKYSECAFFQECQRKLKHRLRL
  DSYLLKPVQRITKYQLLLKELLKYSKDCEGSALLKKALDAMLDLLKSVNDSMHQIAINGYIGNLNELGKM
  IMQGGFSVWIGHKKGATKMKDLARFKPMQRHLFLYEKAIVFCKRRVESGEGSDRYPSYSFKHCWKMDEVG
  ITEYVKGDNRKFEIWYGEKEEVYIVQASNVDVKMTWLKEIRNILLKQQELLTVKKRKQQDQLTERDKFQI
  SLQQNDEKQQGAFISTEETELEHTSTVVEVCEAIASVQAEANTVWTEASQSAEISEEPAEWSSNYFYPTY
  DENEEENRPLMRPVSEMALLY
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001171876
• ORF Size: 3003 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001171876.2
• RefSeq Size: 3867 bp
• RefSeq ORF: 3006 bp
• Locus ID: 4168
• UniProt ID: P10911
• Cytogenetics: Xq27.1
• Protein Families: Druggable Genome
• Gene Summary: The oncogenic protein encoded by this gene is a guanine nucleotide exchange factor (GEF) that exerts control over some members of the Rho family of small GTPases. Several transcript variants encoding different isoforms have been found for this gene. These isoforms exhibit different expression patterns and varying levels of GEF activity.[provided by RefSeq, Jan 2010]