ITIH4 (NM_001166449) Human Tagged ORF Clone

CAT#: RG230577

ITIH4 (tGFP-tagged) - Human inter-alpha (globulin) inhibitor H4 (plasma Kallikrein-sensitive glycoprotein) (ITIH4), transcript variant 2

Product Images

Circular map for RG230577

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ITIH4
• Synonyms: GP120; H4P; IHRP; ITI-HC4; ITIHL1; PK-120; PK120
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG230577 representing NM_001166449
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCCCCCAAGGCCTGTCCGTACCTGCAGCAAAGTTCTCGTCCTGCTTTCACTGCTGGCCATCCACC
  AGACTACTACTGCCGAAAAGAATGGCATCGACATCTACAGCCTCACCGTGGACTCCAGGGTCTCATCCCG
  ATTTGCCCACACGGTCGTCACCAGCCGAGTGGTCAATAGGGCCAATACTGTGCAGGAGGCCACCTTCCAG
  ATGGAGCTGCCCAAGAAAGCCTTCATCACCAACTTCTCCATGATCATCGATGGCATGACCTACCCAGGGA
  TCATCAAGGAGAAGGCTGAAGCCCAGGCACAGTACAGCGCAGCAGTGGCCAAGGGAAAGAGCGCTGGCCT
  CGTCAAGGCCACCGGGAGAAACATGGAGCAGTTCCAGGTGTCGGTCAGTGTGGCTCCCAATGCCAAGATC
  ACCTTTGAGCTGGTCTATGAGGAGCTGCTCAAGCGGCGTTTGGGGGTGTACGAGCTGCTGCTGAAAGTGC
  GGCCCCAGCAGCTGGTCAAGCACCTGCAGATGGACATTCACATCTTCGAGCCCCAGGGCATCAGCTTTCT
  GGAGACAGAGAGCACCTTCATGACCAACCAGCTGGTAGACGCCCTCACCACCTGGCAGAATAAGACCAAG
  GCTCACATCCGGTTCAAGCCAACACTTTCCCAGCAGCAAAAGTCCCCAGAGCAGCAAGAAACAGTCCTGG
  ACGGCAACCTCATTATCCGCTATGATGTGGACCGGGCCATCTCCGGGGGCTCCATTCAGATCGAGAACGG
  CTACTTTGTACACTACTTTGCCCCCGAGGGCCTAACCACAATGCCCAAGAATGTGGTCTTTGTCATTGAC
  AAGAGCGGCTCCATGAGTGGCAGGAAAATCCAGCAGACCCGGGAAGCCCTAATCAAGATCCTGGATGACC
  TCAGCCCCAGAGACCAGTTCAACCTCATCGTCTTCAGTACAGAAGCAACTCAGTGGAGGCCATCACTGGT
  GCCAGCCTCAGCCGAGAACGTGAACAAGGCCAGGAGCTTTGCTGCGGGCATCCAGGCCCTGGGAGGGACC
  AACATCAATGATGCAATGCTGATGGCTGTGCAGTTGCTGGACAGCAGCAACCAGGAGGAGCGGCTGCCCG
  AAGGGAGTGTCTCACTCATCATCCTGCTCACCGATGGCGACCCCACTGTGGGGGAGACTAACCCCAGGAG
  CATCCAGAATAACGTGCGGGAAGCTGTAAGTGGCCGGTACAGCCTCTTCTGCCTGGGCTTCGGTTTCGAC
  GTCAGCTATGCCTTCCTGGAGAAGCTGGCACTGGACAATGGCGGCCTGGCCCGGCGCATCCATGAGGACT
  CAGACTCTGCCCTGCAGCTCCAGGACTTCTACCAGGAAGTGGCCAACCCACTGCTGACAGCAGTGACCTT
  CGAGTACCCAAGCAATGCCGTGGAGGAGGTCACTCAGAACAACTTCCGGCTCCTCTTCAAGGGCTCAGAG
  ATGGTGGTGGCTGGGAAGCTCCAGGACCGGGGGCCTGATGTGCTCACAGCCACAGTCAGTGGGAAGCTGC
  CTACACAGAACATCACTTTCCAAACGGAGTCCAGTGTGGCAGAGCAGGAGGCGGAGTTCCAGAGCCCCAA
  GTATATCTTCCACAACTTCATGGAGAGGCTCTGGGCATACCTGACTATCCAGCAGCTGCTGGAGCAAACT
  GTCTCCGCATCCGATGCTGATCAGCAGGCCCTCCGGAACCAAGCGCTGAATTTATCACTTGCCTACAGCT
  TTGTCACGCCTCTCACATCTATGGTAGTCACCAAACCCGATGACCAAGAGCAGTCTCAAGTTGCTGAGAA
  GCCCATGGAAGGCGAAAGTAGAAACAGGAATGTCCACTCAGCTGGAGCTGCTGGCTCCCGGATGAATTTC
  AGACCTGGGGTTCTCAGCTCCAGGCAACTTGGACTCCCAGGACCTCCTGATGTTCCTGACCATGCTGCTT
  ACCACCCCTTCCGCCGTCTGGCCATCTTGCCTGCTTCAGCACCACCAGCCACCTCAAATCCTGATCCAGC
  TGTGTCTCGTGTCATGAATATGAAAATCGAAGAAACAACCATGACAACCCAAACCCCAGCCCCCATACAG
  GCTCCCTCTGCCATCCTGCCACTGCCTGGGCAGAGTGTGGAGCGGCTCTGTGTGGACCCCAGACACCGCC
  AGGGGCCAGTGAACCTGCTCTCAGACCCTGAGCAAGGGGTTGAGGTGACTGGCCAGTATGAGAGGGAGAA
  GGCTGGGTTCTCATGGATCGAAGTGACCTTCAAGAACCCCCTGGTATGGGTTCACGCATCCCCTGAACAC
  GTGGTGGTGACTCGGAACCGAAGAAGCTCTGCGTACAAGTGGAAGGAGACGCTATTCTCAGTGATGCCCG
  GCCTGAAGATGACCATGGACAAGACGGGTCTCCTGCTGCTCAGTGACCCAGACAAAGTGACCATCGGCCT
  GTTGTTCTGGGATGGCCGTGGGGAGGGGCTCCGGCTCCTTCTGCGTGACACTGACCGCTTCTCCAGCCAC
  GTTGGAGGGACCCTTGGCCAGTTTTACCAGGAGGTGCTCTGGGGATCTCCAGCAGCATCAGATGACGGCA
  GACGCACGCTGAGGGTTCAGGGCAATGACCACTCTGCCACCAGAGAGCGCAGGCTGGATTACCAGGAGGG
  GCCCCCGGGAGTGGAGATTTCCTGCTGGTCTGTGGAGCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG230577 representing NM_001166449
  Red=Cloning site Green=Tags(s)
  
  MKPPRPVRTCSKVLVLLSLLAIHQTTTAEKNGIDIYSLTVDSRVSSRFAHTVVTSRVVNRANTVQEATFQ
  MELPKKAFITNFSMIIDGMTYPGIIKEKAEAQAQYSAAVAKGKSAGLVKATGRNMEQFQVSVSVAPNAKI
  TFELVYEELLKRRLGVYELLLKVRPQQLVKHLQMDIHIFEPQGISFLETESTFMTNQLVDALTTWQNKTK
  AHIRFKPTLSQQQKSPEQQETVLDGNLIIRYDVDRAISGGSIQIENGYFVHYFAPEGLTTMPKNVVFVID
  KSGSMSGRKIQQTREALIKILDDLSPRDQFNLIVFSTEATQWRPSLVPASAENVNKARSFAAGIQALGGT
  NINDAMLMAVQLLDSSNQEERLPEGSVSLIILLTDGDPTVGETNPRSIQNNVREAVSGRYSLFCLGFGFD
  VSYAFLEKLALDNGGLARRIHEDSDSALQLQDFYQEVANPLLTAVTFEYPSNAVEEVTQNNFRLLFKGSE
  MVVAGKLQDRGPDVLTATVSGKLPTQNITFQTESSVAEQEAEFQSPKYIFHNFMERLWAYLTIQQLLEQT
  VSASDADQQALRNQALNLSLAYSFVTPLTSMVVTKPDDQEQSQVAEKPMEGESRNRNVHSAGAAGSRMNF
  RPGVLSSRQLGLPGPPDVPDHAAYHPFRRLAILPASAPPATSNPDPAVSRVMNMKIEETTMTTQTPAPIQ
  APSAILPLPGQSVERLCVDPRHRQGPVNLLSDPEQGVEVTGQYEREKAGFSWIEVTFKNPLVWVHASPEH
  VVVTRNRRSSAYKWKETLFSVMPGLKMTMDKTGLLLLSDPDKVTIGLLFWDGRGEGLRLLLRDTDRFSSH
  VGGTLGQFYQEVLWGSPAASDDGRRTLRVQGNDHSATRERRLDYQEGPPGVEISCWSVEL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001166449
• ORF Size: 2700 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001166449.1, NP_001159921.1
• RefSeq Size: 3193 bp
• RefSeq ORF: 2703 bp
• Locus ID: 3700
• UniProt ID: Q14624
• Cytogenetics: 3p21.1
• Gene Summary: The protein encoded by this gene is secreted into the blood, where it is cleaved by plasma kallikrein into two smaller forms. Expression of this gene has been detected only in liver, and it seems to be upregulated during surgical trauma. This gene is part of a cluster of similar genes on chromosome 3. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]