C3orf20 (NM_001184957) Human Tagged ORF Clone

CAT#: RG230495

C3orf20 (tGFP-tagged) - Human chromosome 3 open reading frame 20 (C3orf20), transcript variant 3

Product Images

Circular map for RG230495

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: C3orf20
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG230495 representing NM_001184957
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCGTCAGGTGCGCACCCACCAGGAGACCCTGAACAGGTTTCAGCAGCAGTCCATCCACCTGCTGA
  CGGAGCTCCTCAGACTGAAGATGAAGGCCATGGTGGAGTCTATGTCGGTGGGTGCCAACCCCTTGGACAT
  CACCAGGCGCTTTGTGGAGGCCAGCCAGCTCCTCCACCTCAATGCCAAGGAGATGGCCTTCAACTGCCTG
  ATCAGCACAGCCGGGAGAAGTGGCTACAGCAGCGGACAGTTGTGGAAAGAGTCCCTCGCAAACATGTCCG
  CCATTGGGGTGAACTCGCCTTACCAGCTGATCTACCACTCTTCCACAGCCTGTCTGAGCTTTTCTCTCTC
  TGCTGGAAAAGAAGCCAAGAAGAAAATAGGCAAATCTAGAACTACAGAAGATGTCAGCATGCCGCCCCTG
  CATCGAGGAGTGGGAACCCCTGCCAACAGCCTGGAGTTCAGCGACCCCTGCCCTGAGGCCCGGGAGAAGC
  TGCAGGAGTTGTGTCGCCACATAGAAGCTGAAAGGGCCACATGGAAAGGGAGGAATATCTCCTACCCCAT
  GATCTTACGAAACTACAAGGCAAAGATGCCCTCTCATCTAATGTTGGCCCGCAAAGGAGACTCTCAGACC
  CCGGGTTTACATTACCCTCCCACTGCAGGTGCTCAGACTCTCAGCCCCACCTCTCACCCATCTTCTGCCA
  ACCATCATTTCAGTCAGCATTGTCAAGAGGGGAAGGCACCCAAGAAGGCCTTCAAGTTTCATTACACCTT
  CTATGATGGCTCCTCCTTCGTTTACTATCCCTCTGGAAACGTCGCTGTATGTCAGATCCCCACATGCTGC
  AGAGGGAGAACCATCACCTGCCTCTTTAATGACATACCTGGATTCTCCTTGCTGGCCCTATTCAATACTG
  AAGGCCAGGGCTGTGTTCACTACAACCTAAAAACCAGTTGCCCATATGTCTTAATCTTGGATGAGGAAGG
  TGGGACCACCAATGACCAGCAGGGCTATGTAGTCCACAAGTGGAGCTGGACTTCCAGGACAGAGACCCTG
  CTTTCCCTGGAATACAAGGTGAATGAGGAAATGAAACTAAAGGTACTGGGACAGGACTCCATCACAGTCA
  CCTTCACCTCCCTGAATGAGACAGTAACACTCACTGTGTCGGCCAACAATTGTCCCCATGGAATGGCATA
  TGACAAACGGCTGAACCGCAGAATCAGCAACATGGACGACAAGGTGTATAAGATGAGCCGAGCCCTGGCT
  GAGATCAAGAAGCGGTTTCAGAAGACAGTGACTCAGTTCATTAATTCTATCTTGCTGGCCGCAGGTCTGT
  TTACCATTGAATATCCCACCAAAAAGGAGGAGGAAGAATTTGTTCGGTTCAAGATGAGATCCAGAACTCA
  TCCCGAGCGGCTCCCCAAGCTAAGTTTATACTCAGGAGAAAGTCTTTTACGATCTCAGTCAGGCCACCTG
  GAATCCTCAATTGCAGAGACTTTGAAGGATGAGCCTGAGTCTGCTCCTGTGAGCCCAGTTCGGAAGACCA
  CCAAAATCCACACCAAAGCCAAGGTCACATCCAGAGGGAAGGCCCGCGAGGGGCGCAGCCCCACCAGGTG
  GGCGGCCTTGCCCTCAGACTGCCCGCTGGTGCTGCGGAAGCTCATGCTCAAGGAAGACACCCGTGCTGGC
  TGCAAGTGCCTGGTGAAGGCGCCCCTGGTCTCTGACGTGGAGCTGGAGCGCTTCCTGTTGGCGCCCCGAG
  ACCCCAGCCAAGTGCTGGTGTTTGGGATCATCTCAAGCCAGAACTACACCAGCACTGGGCAGCTCCAGTG
  GCTGCTGAACACTCTCTACAACCACCAGCAGCGGGGCCGTGGCTCCCCCTGCATCCAGTGCCGGTATGAC
  TCCTACCGCCTGCTGCAGTATGACCTGGACAGCCCCCTGCAGGAGGACCCTCCCCTGATGGTGAAGAAGA
  ACTCTGTGGTGCAGGGGATGATTCTGATGTTTGCCGGGGGGAAGCTCATTTTTGGGGGCCGTGTTTTGAA
  TGGATATGGCCTCAGCAAGCAGAATCTGCTGAAACAGATCTTCCGGTCTCAACAGGATTACAAGATGGGC
  TACTTCCTGCCGGATGACTACAAATTCAGTGTTCCCAACTCTGTCCTGAGCCTGGAGGATTCTGAATCAG
  TCAAGAAAGCCGAGTCAGAAGATATCCAAGGAAGCAGCTCCTCATTGGCCCTGGAAGACTATGTGGAGAA
  GGAGTTATCTCTGGAGGCTGAGAAGACAAGAGAGCCTGAAGTGGAGCTACATCCTCTCAGCAGGGACAGC
  AAGATAACTAGTTGGAAGAAGCAGGCCTCCAAGAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG230495 representing NM_001184957
  Red=Cloning site Green=Tags(s)
  
  MARQVRTHQETLNRFQQQSIHLLTELLRLKMKAMVESMSVGANPLDITRRFVEASQLLHLNAKEMAFNCL
  ISTAGRSGYSSGQLWKESLANMSAIGVNSPYQLIYHSSTACLSFSLSAGKEAKKKIGKSRTTEDVSMPPL
  HRGVGTPANSLEFSDPCPEAREKLQELCRHIEAERATWKGRNISYPMILRNYKAKMPSHLMLARKGDSQT
  PGLHYPPTAGAQTLSPTSHPSSANHHFSQHCQEGKAPKKAFKFHYTFYDGSSFVYYPSGNVAVCQIPTCC
  RGRTITCLFNDIPGFSLLALFNTEGQGCVHYNLKTSCPYVLILDEEGGTTNDQQGYVVHKWSWTSRTETL
  LSLEYKVNEEMKLKVLGQDSITVTFTSLNETVTLTVSANNCPHGMAYDKRLNRRISNMDDKVYKMSRALA
  EIKKRFQKTVTQFINSILLAAGLFTIEYPTKKEEEEFVRFKMRSRTHPERLPKLSLYSGESLLRSQSGHL
  ESSIAETLKDEPESAPVSPVRKTTKIHTKAKVTSRGKAREGRSPTRWAALPSDCPLVLRKLMLKEDTRAG
  CKCLVKAPLVSDVELERFLLAPRDPSQVLVFGIISSQNYTSTGQLQWLLNTLYNHQQRGRGSPCIQCRYD
  SYRLLQYDLDSPLQEDPPLMVKKNSVVQGMILMFAGGKLIFGGRVLNGYGLSKQNLLKQIFRSQQDYKMG
  YFLPDDYKFSVPNSVLSLEDSESVKKAESEDIQGSSSSLALEDYVEKELSLEAEKTREPEVELHPLSRDS
  KITSWKKQASKK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001184957
• ORF Size: 2346 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001184957.2
• RefSeq Size: 2847 bp
• RefSeq ORF: 2349 bp
• Locus ID: 84077
• UniProt ID: Q8ND61
• Cytogenetics: 3p25.1