IGSF10 (NM_001178145) Human Tagged ORF Clone

CAT#: RG230393

IGSF10 (tGFP-tagged) - Human immunoglobulin superfamily, member 10 (IGSF10), transcript variant 2

Product Images

Circular map for RG230393

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: IGSF10
• Synonyms: CMF608
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG230393 representing NM_001178145
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGAAAGCGTAAGGCTTGAGAACCAGCCCTGTGACCTAAGAGTGGGCAGCTGGATCCACGTCTACC
  CTAATGGATCCCTGTTTATTGGATCAGTAACAGAAAAAGACAGTGGTGTCTACTTGTGTGTGGCAAGAAA
  CAAAATGGGGGATGATCTGATACTGATGCATGTTAGCCTAAGACTGAAACCTGCCAAAATTGACCACAAG
  CAGTATTTTAGAAAGCAAGTGCTCCATGGGAAAGATTTCCAAGTAGATTGCAAAGCTTCCGGCTCCCCAG
  TGCCAGAGATATCTTGGAGTTTGCCTGATGGAACCATGATCAACAATGCAATGCAAGCCGATGACAGTGG
  CCACAGGACTAGGAGATATACCCTTTTCAACAATGGAACTTTATACTTCAACAAAGTTGGGGTAGCGGAG
  GAAGGAGATTATACTTGCTATGCCCAGAACACCCTAGGGAAAGATGAAATGAAGGTCCACTTAACAGTTA
  TAACAGCTGCTCCCCGGATAAGGCAGAGTAACAAAACCAACAAGAGAATCAAAGCTGGAGACACAGCTGT
  CCTTGACTGTGAGGTCACTGGGGATCCCAAACCAAAAATATTTTGGTTGCTGCCTTCCAATGACATGATT
  TCCTTCTCCATTGATAGGTACACATTTCATGCCAATGGGTCTTTGACCATCAACAAAGTGAAACTGCTCG
  ATTCTGGAGAGTACGTATGTGTAGCCCGAAATCCCAGTGGGGATGACACCAAAATGTACAAACTGGATGT
  GGTCTCTAAACCTCCATTAATCAATGGTCTGTATACAAACAGAACTGTTATTAAAGCCACAGCTGTGAGA
  CATTCCAAAAAACACTTTGACTGCAGAGCTGAAGGGACACCATCTCCTGAAGTCATGTGGATCATGCCAG
  ACAATATTTTCCTCACAGCCCCATACTATGGAAGCAGAATCACAGTCCATAAAAATGGAACCTTGGAAAT
  TAGGAATGTGAGGCTTTCAGATTCAGCCGACTTTATCTGTGTGGCCCGAAATGAAGGTGGAGAGAGCGTG
  TTGGTAGTACAGTTAGAAGTACTGGAAATGCTGAGAAGACCGACATTTAGAAATCCATTTAATGAAAAAA
  TAGTTGCCCAGCTGGGAAAGTCCACAGCATTGAATTGCTCTGTTGATGGTAACCCACCACCTGAAATAAT
  CTGGATTTTACCAAATGGCACACGATTTTCCAATGGACCACAAAGTTATCAGTATCTGATAGCAAGCAAT
  GGTTCTTTTATCATTTCTAAAACAACTCGGGAGGATGCAGGAAAATATCGCTGTGCAGCTAGGAATAAAG
  TTGGCTATATTGAGAAATTAGTCATATTAGAAATTGGCCAGAAGCCAGTTATTCTTACCTATGCACCAGG
  GACAGTAAAAGGCATCAGTGGAGAATCTCTATCACTGCATTGTGTGTCTGATGGAATCCCTAAGCCAAAT
  ATCAAATGGACTATGCCAAGTGGTTATGTAGTAGACAGGCCTCAAATTAATGGGAAATACATATTGCATG
  ACAATGGCACCTTAGTCATTAAAGAAGCAACAGCTTATGACAGAGGAAACTATATCTGTAAGGCTCAAAA
  TAGTGTTGGTCATACACTGATTACTGTTCCAGTAATGATTGTAGCCTACCCTCCCCGAATTACAAATCGT
  CCACCCAGGAGTATTGTCACCAGGACAGGGGCAGCCTTTCAGCTCCACTGTGTGGCCTTGGGAGTTCCCA
  AGCCAGAAATCACATGGGAGATGCCTGACCACTCCCTTCTCTCAACGGCAAGTAAAGAGAGGACACATGG
  AAGTGAGCAGCTTCACTTACAAGGTACCCTAGTCATTCAGAATCCCCAAACCTCCGATTCTGGGATATAC
  AAATGCACAGCAAAGAACCCACTTGGTAGTGATTATGCAGCAACGTATATTCAAGTAATC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG230393 representing NM_001178145
  Red=Cloning site Green=Tags(s)
  
  MVESVRLENQPCDLRVGSWIHVYPNGSLFIGSVTEKDSGVYLCVARNKMGDDLILMHVSLRLKPAKIDHK
  QYFRKQVLHGKDFQVDCKASGSPVPEISWSLPDGTMINNAMQADDSGHRTRRYTLFNNGTLYFNKVGVAE
  EGDYTCYAQNTLGKDEMKVHLTVITAAPRIRQSNKTNKRIKAGDTAVLDCEVTGDPKPKIFWLLPSNDMI
  SFSIDRYTFHANGSLTINKVKLLDSGEYVCVARNPSGDDTKMYKLDVVSKPPLINGLYTNRTVIKATAVR
  HSKKHFDCRAEGTPSPEVMWIMPDNIFLTAPYYGSRITVHKNGTLEIRNVRLSDSADFICVARNEGGESV
  LVVQLEVLEMLRRPTFRNPFNEKIVAQLGKSTALNCSVDGNPPPEIIWILPNGTRFSNGPQSYQYLIASN
  GSFIISKTTREDAGKYRCAARNKVGYIEKLVILEIGQKPVILTYAPGTVKGISGESLSLHCVSDGIPKPN
  IKWTMPSGYVVDRPQINGKYILHDNGTLVIKEATAYDRGNYICKAQNSVGHTLITVPVMIVAYPPRITNR
  PPRSIVTRTGAAFQLHCVALGVPKPEITWEMPDHSLLSTASKERTHGSEQLHLQGTLVIQNPQTSDSGIY
  KCTAKNPLGSDYAATYIQVI
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001178145
• ORF Size: 1950 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001178145.2
• RefSeq Size: 2824 bp
• RefSeq ORF: 1809 bp
• Locus ID: 285313
• UniProt ID: Q6WRI0
• Cytogenetics: 3q25.1
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: Involved in the control of early migration of neurons expressing gonadotropin-releasing hormone (GNRH neurons) (By similarity). May be involved in the maintenance of osteochondroprogenitor cells pool (By similarity).[UniProtKB/Swiss-Prot Function]