SSX2IP (NM_001166293) Human Tagged ORF Clone

CAT#: RG230350

SSX2IP (tGFP-tagged) - Human synovial sarcoma, X breakpoint 2 interacting protein (SSX2IP), transcript variant 2

Product Images

Circular map for RG230350

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SSX2IP
• Synonyms: ADIP; hMsd1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG230350 representing NM_001166293
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGATTGGATGACTGTTACAGATCCAGGTCTGTCTTCAGAAAGCAAAACTATCTCTCAATATACCT
  CAGAAACAAAGATGTCTCCATCAAGTTTATACTCACAGCAAGTGCTATGTTCTTCAATACCTTTATCGAA
  AAATGTGCACAGTTTTTTCAGTGCCTTCTGCACAGAAGATAATATTGAACAGAGTATCTCATATCTTGAT
  CAGGAATTGACTACTTTTGGTTTTCCTTCATTATATGAAGAATCCAAAGGTAAAGAGACAAAGAGAGAGT
  TAAATATAGTAGCTGTACTAAATTGTATGAATGAGCTGCTTGTGCTTCAGCGGAAGAACCTTCTAGCTCA
  GGAAAATGTGGAGACACAGAATTTGAAGCTGGGAAGTGATATGGACCATCTACAGAGCTGCTACTCAAAA
  CTTAAGGAACAACTGGAAACCTCCAGGAGGGAAATGATTGGGCTTCAGGAAAGAGACAGACAGTTACAAT
  GTAAGAACAGGAATTTGCATCAGCTACTAAAGAATGAGAAAGATGAGGTGCAAAAATTACAAAATATCAT
  TGCAAGTCGAGCTACTCAGTATAATCATGATATGAAGAGAAAAGAGCGTGAATATAATAAACTGAAGGAA
  CGTCTACATCAACTTGTTATGAACAAGAAAGATAAGAAAATAGCTATGGACATTTTGAATTATGTCGGGA
  GAGCTGATGGAAAAAGAGGCTCCTGGAGGACTGGTAAAACTGAAGCCAGGAATGAAGATGAAATGTATAA
  AATTCTCTTGAATGATTATGAATATCGTCAGAAACAAATCCTAATGGAAAATGCAGAACTTAAGAAGGTT
  CTTCAACAAATGAAAAAGGAAATGATTTCTCTTCTTTCTCCCCAAAAGAAGAAACCTAGAGAAAGAGTAG
  ATGATAGTACAGGAACTGTTATTTCCGATGTTGAAGAAGATGCCGGGGAACTAAGCAGAGAGAGTATGTG
  GGACCTTTCCTGTGAAACTGTGAGAGAGCAGCTTACAAACAGCATCAGAAAACAGTGGAGAATTTTGAAA
  AGTCATGTAGAAAAGCTTGATAACCAAGTTTCAAAGGTACACCTGGAAGGTTTTAATGATGAAGATGTAA
  TCTCACGACAAGACCATGAACAAGAAACTGAAAAACTCGAGTTAGAAATTCAGCAGTGTAAAGAAATGAT
  TAAAACTCAGCAACAGCTTTTACAGCAGCAGCTCGCTACTGCATATGATGATGATACCACTTCACTATTA
  CGAGACTGTTATTTGTTGGAAGAAAAGGAACGTCTCAAAGAAGAATGGTCCCTTTTTAAAGAGCAGAAAA
  AGAATTTTGAGAGGGAGAGACGAAGCTTTACAGAAGCCGCTATTCGCCTGGGATTGGAGAGAAAGGCATT
  TGAAGAAGAAAGAGCCAGTTGGTTAAAGCAGCAGTTTCTAAATATGACTACCTTTGACCACCAGAACTCA
  GAAAATGTGAAACTTTTCAGTGCCTTCTCAGGAAGTTCTGATTGGGACAATCTTATAGTGCACTCGAGGC
  AGCCGCAAAAGAAGCCTCACAGTGTGTCTAATGGGTCTCCAGTTTGCATGTCTAAACTTACTAAATCTCT
  TCCTGCTTCACCTTCCACTTCAGACTTTTGCCAGACACGTTCCTGCATATCTGAACATAGTTCAATCAAT
  GTACTGAATATAACTGCTGAAGAAATTAAACCAAATCAGGTTGGAGGAGAATGTACAAATCAAAAATGGA
  GTGTGGCATCAAGACCTGGATCACAGGAAGGTTGCTATAGTGGATGCTCCTTGAGCTACACAAATTCTCA
  TGTAGAAAAAGATGACTTACCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG230350 representing NM_001166293
  Red=Cloning site Green=Tags(s)
  
  MGDWMTVTDPGLSSESKTISQYTSETKMSPSSLYSQQVLCSSIPLSKNVHSFFSAFCTEDNIEQSISYLD
  QELTTFGFPSLYEESKGKETKRELNIVAVLNCMNELLVLQRKNLLAQENVETQNLKLGSDMDHLQSCYSK
  LKEQLETSRREMIGLQERDRQLQCKNRNLHQLLKNEKDEVQKLQNIIASRATQYNHDMKRKEREYNKLKE
  RLHQLVMNKKDKKIAMDILNYVGRADGKRGSWRTGKTEARNEDEMYKILLNDYEYRQKQILMENAELKKV
  LQQMKKEMISLLSPQKKKPRERVDDSTGTVISDVEEDAGELSRESMWDLSCETVREQLTNSIRKQWRILK
  SHVEKLDNQVSKVHLEGFNDEDVISRQDHEQETEKLELEIQQCKEMIKTQQQLLQQQLATAYDDDTTSLL
  RDCYLLEEKERLKEEWSLFKEQKKNFERERRSFTEAAIRLGLERKAFEEERASWLKQQFLNMTTFDHQNS
  ENVKLFSAFSGSSDWDNLIVHSRQPQKKPHSVSNGSPVCMSKLTKSLPASPSTSDFCQTRSCISEHSSIN
  VLNITAEEIKPNQVGGECTNQKWSVASRPGSQEGCYSGCSLSYTNSHVEKDDLP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001166293
• ORF Size: 1842 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001166293.2
• RefSeq Size: 5847 bp
• RefSeq ORF: 1845 bp
• Locus ID: 117178
• UniProt ID: Q9Y2D8
• Cytogenetics: 1p22.3
• Protein Pathways: Adherens junction
• Gene Summary: This gene encodes a protein that binds the cancer-testis antigen Synovial Sarcoma X breakpoint 2 protein. The encoded protein may regulate the activity of Synovial Sarcoma X breakpoint 2 protein in malignant cells. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 3. [provided by RefSeq, Oct 2009]