SIGLEC10 (NM_001171158) Human Tagged ORF Clone
CAT#: RG230263
- TrueORF®
SIGLEC10 (tGFP-tagged) - Human sialic acid binding Ig-like lectin 10 (SIGLEC10), transcript variant 4
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_001171158" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | SIGLEC10 |
Synonyms | PRO940; SIGLEC-10; SLG2 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG230263 representing NM_001171158
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTACTGCCACTGCTGCTGTCCTCGCTGCTGGGCGGGTCCCAGGCTATGGATGGGAGATTCTGGATAC GAGTGCAGGAGTCAGTGATGGTGCCGGAGGGCCTGTGCATCTCTGTGCCCTGCTCTTTCTCCTACCCCCG ACAAGACTGGACAGGGTCTACCCCAGCTTATGGCTACTGGTTCAAAGCAGTGACTGAGACAACCAAGGGT GCTCCTGTGGCCACAAACCACCAGAGTCGAGAGGTGGAAATGAGCACCCGGGGCCGATTCCAGCTCACTG GGGATCCCGCCAAGGGGAACTGCTCCTTGGTGATCAGAGACGCGCAGATGCAGGATGAGTCACAGTACTT CTTTCGGGTGGAGAGAGGAAGCTATGTGAGATATAATTTCATGAACGATGGGTTCTTTCTAAAAGTAACA GGAACCAAACCAACGACCTCCCACTTCTCAGTGCTCAGCTTCACGCCCAGACCCCAGGACCACAACACCG ACCTCACCTGCCATGTGGACTTCTCCAGAAAGGGTGTGAGCGCACAGAGGACCGTCCGACTCCGTGTGGC CTATGCCCCCAGAGACCTTGTTATCAGCATTTCACGTGACAACACGCCAGCCCTGGAGCCCCAGCCCCAG GGAAATGTCCCATACCTGGAAGCCCAAAAAGGCCAGTTCCTGCGGCTCCTCTGTGCTGCTGACAGCCAGC CCCCTGCCACACTGAGCTGGGTCCTGCAGAACAGAGTCCTCTCCTCGTCCCATCCCTGGGGCCCTAGACC CCTGGGGCTGGAGCTGCCCGGGGTGAAGGCTGGGGATTCAGGGCGCTACACCTGCCGAGCGGAGAACAGG CTTGGCTCCCAGCAGCGAGCCCTGGACCTCTCTGTGCAGTATCCTCCAGAGAACCTGAGAGTGATGGTTT CCCAAGCAAACAGGACAGTCCTGGAAAACCTTGGGAACGGCACGTCTCTCCCAGTACTGGAGGGCCAAAG CCTGTGCCTGGTCTGTGTCACACACAGCAGCCCCCCAGCCAGGCTGAGCTGGACCCAGAGGGGACAGGTT CTGAGCCCCTCCCAGCCCTCAGACCCCGGGGTCCTGGAGCTGCCTCGGGTTCAAGTGGAGCACGAAGGAG AGTTCACCTGCCACGCTCGGCACCCACTGGGCTCCCAGCACGTCTCTCTCAGCCTCTCCGTGCACTATAA GAAGGGACTCATCTCAACGGCATTCTCCAACGGAGCGTTTCTGGGAATCGGCATCACGGCTCTTCTTTTC CTCTGCCTGGCCCTGATCATCATGAAGATTCTACCGAAGAGACGGACTCAGACAGAAACCCCGAGGCCCA GGTTCTCCCGGCACAGCACGATCCTGGATTACATCAATGTGGTCCCGACGGCTGGCCCCCTGGCTCAGAA GCGGAATCAGAAAGCCACACCAAACAGTCCTCGGACCCCTCTTCCACCAGGTGCTCCCTCCCCAGAATCA AAGAAGAACCAGAAAAAGCAGTATCAGTTGCCCAGTTTCCCAGAACCCAAATCATCCACTCAAGCCCCAG AATCCCAGGAGAGCCAAGAGGAGCTCCATTATGCCACGCTCAACTTCCCAGGCGTCAGACCCAGGCCTGA GGCCCGGATGCCCAAGGGCACCCAGGCGGATTATGCAGAAGTCAAGTTCCAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG230263 representing NM_001171158
Red=Cloning site Green=Tags(s) MLLPLLLSSLLGGSQAMDGRFWIRVQESVMVPEGLCISVPCSFSYPRQDWTGSTPAYGYWFKAVTETTKG APVATNHQSREVEMSTRGRFQLTGDPAKGNCSLVIRDAQMQDESQYFFRVERGSYVRYNFMNDGFFLKVT GTKPTTSHFSVLSFTPRPQDHNTDLTCHVDFSRKGVSAQRTVRLRVAYAPRDLVISISRDNTPALEPQPQ GNVPYLEAQKGQFLRLLCAADSQPPATLSWVLQNRVLSSSHPWGPRPLGLELPGVKAGDSGRYTCRAENR LGSQQRALDLSVQYPPENLRVMVSQANRTVLENLGNGTSLPVLEGQSLCLVCVTHSSPPARLSWTQRGQV LSPSQPSDPGVLELPRVQVEHEGEFTCHARHPLGSQHVSLSLSVHYKKGLISTAFSNGAFLGIGITALLF LCLALIIMKILPKRRTQTETPRPRFSRHSTILDYINVVPTAGPLAQKRNQKATPNSPRTPLPPGAPSPES KKNQKKQYQLPSFPEPKSSTQAPESQESQEELHYATLNFPGVRPRPEARMPKGTQADYAEVKFQ TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001171158 |
ORF Size | 1662 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001171158.1, NP_001164629.1 |
RefSeq Size | 2971 bp |
RefSeq ORF | 1665 bp |
Locus ID | 89790 |
UniProt ID | Q96LC7 |
Cytogenetics | 19q13.41 |
Protein Families | Druggable Genome, Transmembrane |
Gene Summary | SIGLECs are members of the immunoglobulin superfamily that are expressed on the cell surface. Most SIGLECs have 1 or more cytoplasmic immune receptor tyrosine-based inhibitory motifs, or ITIMs. SIGLECs are typically expressed on cells of the innate immune system, with the exception of the B-cell expressed SIGLEC6 (MIM 604405).[supplied by OMIM, Jul 2002] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC230263 | SIGLEC10 (Myc-DDK-tagged)-Human sialic acid binding Ig-like lectin 10 (SIGLEC10), transcript variant 4 |
USD 516.00 |
|
RC230263L3 | Lenti-ORF clone of SIGLEC10 (Myc-DDK-tagged)-Human sialic acid binding Ig-like lectin 10 (SIGLEC10), transcript variant 4 |
USD 816.00 |
|
RC230263L4 | Lenti-ORF clone of SIGLEC10 (mGFP-tagged)-Human sialic acid binding Ig-like lectin 10 (SIGLEC10), transcript variant 4 |
USD 816.00 |
|
SC328901 | SIGLEC10 (untagged)-Human sialic acid binding Ig-like lectin 10 (SIGLEC10) transcript variant 4 |
USD 568.00 |
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