CHRNA3 (NM_001166694) Human Tagged ORF Clone
CAT#: RG230168
- TrueORF®
CHRNA3 (tGFP-tagged) - Human cholinergic receptor, nicotinic, alpha 3 (CHRNA3), transcript variant 2
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_001166694" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | CHRNA3 |
Synonyms | BAIPRCK; LNCR2; NACHRA3; PAOD2 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG230168 representing NM_001166694
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCTCTGGCCCGCTCTCGCTGCCCCTGGCGCTGTCGCCGCCGCGGCTGCTGCTGCTGCTGCTGCTGT CTCTGCTGCCAGTGGCCAGGGCCTCAGAGGCTGAGCACCGTCTATTTGAGCGGCTGTTTGAAGATTACAA TGAGATCATCCGGCCTGTAGCCAACGTGTCTGACCCAGTCATCATCCATTTCGAGGTGTCCATGTCTCAG CTGGTGAAGGTGGATGAAGTAAACCAGATCATGGAGACCAACCTGTGGCTCAAGCAAATCTGGAATGACT ACAAGCTGAAATGGAACCCCTCTGACTATGGTGGGGCAGAGTTCATGCGTGTCCCTGCACAGAAGATCTG GAAGCCAGACATTGTGCTGTATAACAATGCTGTTGGGGATTTCCAGGTGGACGACAAGACCAAAGCCTTA CTCAAGTACACTGGGGAGGTGACTTGGATACCTCCGGCCATCTTTAAGAGCTCCTGTAAAATCGACGTGA CCTACTTCCCGTTTGATTACCAAAACTGTACCATGAAGTTCGGTTCCTGGTCCTACGATAAGGCGAAAAT CGATCTGGTCCTGATCGGCTCTTCCATGAACCTCAAGGACTATTGGGAGAGCGGCGAGTGGGCCATCATC AAAGCCCCAGGCTACAAACACGACATCAAGTACAACTGCTGCGAGGAGATCTACCCCGACATCACATACT CGCTGTACATCCGGCGCCTGCCCTTGTTCTACACCATCAACCTCATCATCCCCTGCCTGCTCATCTCCTT CCTCACTGTGCTCGTCTTCTACCTGCCCTCCGACTGCGGTGAGAAGGTGACCCTGTGCATTTCTGTCCTC CTCTCCCTGACGGTGTTTCTCCTGGTGATCACTGAGACCATCCCTTCCACCTCGCTGGTCATCCCCCTGA TTGGAGAGTACCTCCTGTTCACCATGATTTTTGTAACCTTGTCCATCGTCATCACCGTCTTCGTGCTCAA CGTGCACTACAGAACCCCGACGACACACACAATGCCCTCATGGGTGAAGACTGTATTCTTGAACCTGCTC CCCAGGGTCATGTTCATGACCAGGCCAACAAGCAACGAGGGCAACGCTCAGAAGCCGAGGCCCCTCTACG GTGCCGAGCTCTCAAATCTGAATTGCTTCAGCCGCGCAGAGTCCAAAGGCTGCAAGGAGGGCTACCCCTG CCAGGACGGGATGTGTGGTTACTGCCACCACCGCAGGATAAAAATCTCCAATTTCAGTGCTAACCTCACG AGAAGCTCTAGTTCTGAATCTGTTGATGCTGTGCTGTCCCTCTCTGCTTTGTCACCAGAAATCAAAGAAG CCATCCAAAGTGTCAAGTATATTGCTGAAAATATGAAAGCACAAAATGAAGCCAAAGAGGAACAAAAAGC CCAAGAGATCCAACAATTGAAACGAAAAGAAAAGTCCACAGAAACATCCGATCAAGAACCTGGGCTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG230168 representing NM_001166694
Red=Cloning site Green=Tags(s) MGSGPLSLPLALSPPRLLLLLLLSLLPVARASEAEHRLFERLFEDYNEIIRPVANVSDPVIIHFEVSMSQ LVKVDEVNQIMETNLWLKQIWNDYKLKWNPSDYGGAEFMRVPAQKIWKPDIVLYNNAVGDFQVDDKTKAL LKYTGEVTWIPPAIFKSSCKIDVTYFPFDYQNCTMKFGSWSYDKAKIDLVLIGSSMNLKDYWESGEWAII KAPGYKHDIKYNCCEEIYPDITYSLYIRRLPLFYTINLIIPCLLISFLTVLVFYLPSDCGEKVTLCISVL LSLTVFLLVITETIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHYRTPTTHTMPSWVKTVFLNLL PRVMFMTRPTSNEGNAQKPRPLYGAELSNLNCFSRAESKGCKEGYPCQDGMCGYCHHRRIKISNFSANLT RSSSSESVDAVLSLSALSPEIKEAIQSVKYIAENMKAQNEAKEEQKAQEIQQLKRKEKSTETSDQEPGL TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001166694 |
ORF Size | 1467 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001166694.2 |
RefSeq Size | 2040 bp |
RefSeq ORF | 1470 bp |
Locus ID | 1136 |
UniProt ID | P32297 |
Cytogenetics | 15q25.1 |
Protein Families | Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane |
Gene Summary | This locus encodes a member of the nicotinic acetylcholine receptor family of proteins. Members of this family of proteins form pentameric complexes comprised of both alpha and beta subunits. This locus encodes an alpha-type subunit, as it contains characteristic adjacent cysteine residues. The encoded protein is a ligand-gated ion channel that likely plays a role in neurotransmission. Polymorphisms in this gene have been associated with an increased risk of smoking initiation and an increased susceptibility to lung cancer. Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2009] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC230168 | CHRNA3 (Myc-DDK-tagged)-Human cholinergic receptor, nicotinic, alpha 3 (CHRNA3), transcript variant 2 |
USD 503.00 |
|
RC230168L3 | Lenti-ORF clone of CHRNA3 (Myc-DDK-tagged)-Human cholinergic receptor, nicotinic, alpha 3 (CHRNA3), transcript variant 2 |
USD 803.00 |
|
RC230168L4 | Lenti-ORF clone of CHRNA3 (mGFP-tagged)-Human cholinergic receptor, nicotinic, alpha 3 (CHRNA3), transcript variant 2 |
USD 803.00 |
|
SC328806 | CHRNA3 (untagged)-Human cholinergic receptor nicotinic alpha 3 (CHRNA3) transcript variant 2 |
USD 457.00 |
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