PP11 (ENDOU) (NM_001172440) Human Tagged ORF Clone

CAT#: RG229923

ENDOU (tGFP-tagged) - Human endonuclease, polyU-specific (ENDOU), transcript variant 3

Product Images

Circular map for RG229923

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PP11
• Synonyms: P11; PP11; PRSS26
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG229923 representing NM_001172440
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGGCCTGCATCTCCCTGGTATTGGCCGTGCTGTGTGGCCTGGCCTGGGCTGACTTGTACTCGGCAC
  CCACCTCCTGCCAGGGCCGCTGCTACGAAGCCTTTGACAAGCACCACCAATGTCACTGCAATGCCCGCTG
  CCAAGAGTTTGGGAACTGCTGCAAGGATTTTGAGAGCCTGTGTAGTGACCACGAGGTCTCCCACAGCAGT
  GATGCCATAACAAAAGAGGAGATTCAGAGCATCTCTGAGAAGATCTACAGGGCAGACACCAACAAAGCCC
  AGAAGGAAGACATCGTTCTCAATAGCCAAAACTGCATCTCCCCGTCAGAGACCAGAAACCAAGTGGATCG
  CTGCCCAAAGCCACTCTTCACTTATGTCAATGAGAAGCTGTTCTCCAAGCCCACCTATGCAGCCTTCATC
  AACCTCCTCAACAACTACCAGCGGGCAACAGGCCATGGGGAGCACTTCAGTGCCCAGGAGCTGGCCGAGC
  AGGACGCCTTCCTCAGAGAGATCATGAAGACAGCAGTCATGAAGGAGCTCTACAGCTTCCTCCATCACCA
  GAATCGCTATGGCTCAGAGCAAGAGTTTGTCGATGACTTGAAGAACATGTGGTTTGGGCTCTATTCAAGA
  GGCAATGAAGAGGGGGACTCGAGTGGCTTTGAACATGTCTTCTCAGGTGAGGTAAAAAAAGGCAAGGTTA
  CTGGCTTCCATAACTGGATCCGCTTCTACCTGGAGGAGAAGGAGGGTCTGGTTGACTATTACAGTCACAT
  CTACGATGGGCCTTGGGATTCTTACCCCGATGTGCTGGCAATGCAGTTCAACTGGGACGGCTACTATAAG
  GAAGTGGGCTCTGCTTTCATCGGCAGCAGCCCTGAGTTTGAGTTTGCACTCTACTCCCTGTGCTTCATCG
  CCAGGCCAGGCAAAGTGTGCCAGTTAAGCCTGGGAGGATATCCCTTAGCTGTCCGGACATATACCTGGGA
  CAAGTCCACCTATGGGAATGGCAAGAAGTACATCGCCACAGCCTACATAGTGTCTTCCACC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG229923 representing NM_001172440
  Red=Cloning site Green=Tags(s)
  
  MRACISLVLAVLCGLAWADLYSAPTSCQGRCYEAFDKHHQCHCNARCQEFGNCCKDFESLCSDHEVSHSS
  DAITKEEIQSISEKIYRADTNKAQKEDIVLNSQNCISPSETRNQVDRCPKPLFTYVNEKLFSKPTYAAFI
  NLLNNYQRATGHGEHFSAQELAEQDAFLREIMKTAVMKELYSFLHHQNRYGSEQEFVDDLKNMWFGLYSR
  GNEEGDSSGFEHVFSGEVKKGKVTGFHNWIRFYLEEKEGLVDYYSHIYDGPWDSYPDVLAMQFNWDGYYK
  EVGSAFIGSSPEFEFALYSLCFIARPGKVCQLSLGGYPLAVRTYTWDKSTYGNGKKYIATAYIVSST
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001172440
• ORF Size: 1041 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001172440.2
• RefSeq Size: 2285 bp
• RefSeq ORF: 1044 bp
• Locus ID: 8909
• UniProt ID: P21128
• Cytogenetics: 12q13.11
• Protein Families: Protease
• Gene Summary: This gene encodes a protein with endoribonuclease activity that binds polyuridine-enriched single-stranded RNA. This gene was initially characterized based on its high expression in placenta but was mischaracterized as a serine protease. In mouse, this gene promotes tolerance to self-antigens by regulating B cell activation-induced cell death (AICD). The protein may be useful as a tumor marker. Multiple alternatively spliced transcript variants encoding distinct protein isoforms have been found for this gene. [provided by RefSeq, Jul 2020]