MTF2 (NM_001164391) Human Tagged ORF Clone

CAT#: RG228406

MTF2 (tGFP-tagged) - Human metal response element binding transcription factor 2 (MTF2), transcript variant 3

Product Images

Circular map for RG228406

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MTF2
• Synonyms: dJ976O13.2; M96; PCL2; TDRD19A
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG228406 representing NM_001164391
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCTGTACAATATGTCAAGAAGAGTATTCAGAAGCTCCCAATGAAATGGTTATATGTGACAAGTGTG
  GCCAAGGATATCATCAGTTGTGTCACACACCTCATATTGATTCCAGTGTGATTGATTCAGATGAAAAATG
  GCTCTGTCGGCAGTGTGTTTTTGCAACAACAACAAAGAGGGGTGGTGCACTTAAGAAAGGACCAAATGCC
  AAAGCATTGCAAGTCATGAAGCAGACATTACCCTATAGTGTGGCAGACCTTGAATGGGATGCAGGTCATA
  AAACCAATGTCCAGCAGTGTTACTGCTATTGTGGAGGCCCTGGAGACTGGTATTTGAAGATGCTACAGTG
  CTGCAAATGTAAGCAGTGGTTTCATGAGGCTTGTGTGCAATGCCTTCAAAAGCCAATGCTATTTGGAGAC
  AGATTTTATACGTTTATATGCTCTGTCTGCAGTTCTGGACCAGAATACCTCAAACGTCTACCATTACAGT
  GGGTAGATATAGCACACCTATGCCTTTACAACCTAAGTGTTATTCATAAGAAGAAATACTTTGATTCTGA
  ACTTGAGCTTATGACATACATTAATGAAAACTGGGATAGATTGCACCCTGGAGAGCTGGCAGACACACCA
  AAATCTGAAAGATATGAGCATGTTCTGGAGGCATTAAATGATTACAAGACCATGTTTATGTCTGGGAAAG
  AAATAAAGAAGAAGAAGCATTTGTTTGGGTTGCGAATTCGTGTTCCTCCTGTGCCACCAAATGTGGCTTT
  CAAAGCAGAGAAAGAACCTGAAGGAACATCTCATGAATTTAAAATTAAAGGCAGAAAGGCATCCAAACCT
  ATATCTGATTCAAGGGAAGTAAGCAATGGCATAGAAAAAAAAGGAAAGAAAAAATCTGTAGGTCGTCCAC
  CTGGCCCATATACAAGAAAAATGATTCAAAAAACTGCTGAGCCACTTTTGGATAAGGAATCAATTTCAGA
  GAATCCTACTTTGGATTTACCTTGTTCTATAGGGAGAACTGAGGGAACTGCACATTCATCCAATACCTCA
  GATGTGGATTTCACGGGTGCTTCCAGTGCAAAAGAAACTACCTCGTCTAGCATTTCCAGGCATTATGGAT
  TATCTGACTCCAGAAAAAGAACGCGTACAGGAAGATCTTGGCCTGCTGCAATACCACATTTGCGGAGAAG
  AAGAGGTCGTCTTCCAAGAAGAGCACTCCAGACTCAGAACTCAGAAATTGTAAAAGATGATGAAGGCAAA
  GAAGATTATCAGTTTGATGAACTCAACACAGAGATTCTGAATAACTTAGCAGATCAGGAGTTACAACTCA
  ATCATCTAAAGAACTCCATTACCAGTTATTTTGGTGCTGCAGGTAGAATAGCATGTGGCGAAAAATACCG
  AGTTTTGGCACGTCGGGTGACACTTGATGGAAAGGTGCAGTATCTTGTGGAATGGGAAGGAGCAACTGCA
  TCC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG228406 representing NM_001164391
  Red=Cloning site Green=Tags(s)
  
  MVCTICQEEYSEAPNEMVICDKCGQGYHQLCHTPHIDSSVIDSDEKWLCRQCVFATTTKRGGALKKGPNA
  KALQVMKQTLPYSVADLEWDAGHKTNVQQCYCYCGGPGDWYLKMLQCCKCKQWFHEACVQCLQKPMLFGD
  RFYTFICSVCSSGPEYLKRLPLQWVDIAHLCLYNLSVIHKKKYFDSELELMTYINENWDRLHPGELADTP
  KSERYEHVLEALNDYKTMFMSGKEIKKKKHLFGLRIRVPPVPPNVAFKAEKEPEGTSHEFKIKGRKASKP
  ISDSREVSNGIEKKGKKKSVGRPPGPYTRKMIQKTAEPLLDKESISENPTLDLPCSIGRTEGTAHSSNTS
  DVDFTGASSAKETTSSSISRHYGLSDSRKRTRTGRSWPAAIPHLRRRRGRLPRRALQTQNSEIVKDDEGK
  EDYQFDELNTEILNNLADQELQLNHLKNSITSYFGAAGRIACGEKYRVLARRVTLDGKVQYLVEWEGATA
  S
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001164391
• ORF Size: 1473 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001164391.1, NP_001157863.1
• RefSeq Size: 4064 bp
• RefSeq ORF: 1476 bp
• Locus ID: 22823
• UniProt ID: Q9Y483
• Cytogenetics: 1p22.1
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: Polycomb group (PcG) that specifically binds histone H3 trimethylated at 'Lys-36' (H3K36me3) and recruits the PRC2 complex. Acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting the PRC2 complex, leading to enhance PRC2 H3K27me3 methylation activity. Regulates the transcriptional networks during embryonic stem cell self-renewal and differentiation. Promotes recruitment of the PRC2 complex to the inactive X chromosome in differentiating XX ES cells and PRC2 recruitment to target genes in undifferentiated ES cells. Required to repress Hox genes by enhancing H3K27me3 methylation of the PRC2 complex. In some conditions may act as an inhibitor of PRC2 activity: able to activate the CDKN2A gene and promote cellular senescence by suppressing the catalytic activity of the PRC2 complex locally. Binds to the metal-regulating-element (MRE) of MT1A gene promoter (By similarity).[UniProtKB/Swiss-Prot Function]