LYK5 (STRADA) (NM_001165970) Human Tagged ORF Clone

CAT#: RG228232

STRADA (tGFP-tagged) - Human STE20-related kinase adaptor alpha (STRADA), transcript variant 6

Product Images

Circular map for RG228232

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: LYK5
• Synonyms: LYK5; NY-BR-96; PMSE; Stlk; STRAD; STRAD alpha
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG228232 representing NM_001165970
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCATTTCTTGTAAGTAAACCAGAGCGAATCAGGCGGTGGGTCTCGGAAAAGTTCATTGTTGAGGGCT
  TAAGAGATTTGGAACTATTTGGAGGCAAAGGATTTGAGGACCTGATGACTGTGAATCTAGCAAGGTACAA
  ACCAACAGGAGAGTACGTGACTGTACGGAGGATTAACCTAGAAGCTTGTTCCAATGAGATGGTAACATTC
  TTGCAGGGCGAGCTGCATGTCTCCAAACTCTTCAACCATCCCAATATCGTGCCATATCGAGCCACTTTTA
  TTGCAGACAATGAGCTGTGGGTTGTCACATCATTCATGGCATACGGTTCTGCAAAAGATCTCATCTGTAC
  ACACTTCATGGATGGCATGAATGAGCTGGCGATTGCTTACATCCTGCAGGGGGTGCTGAAGGCCCTCGAC
  TACATCCACCACATGGGATATGTACACAGGAGTGTCAAAGCCAGCCACATCCTGATCTCTGTGGATGGGA
  AGGTCTACCTGTCTGGTTTGCGCAGCAACCTCAGCATGATAAGCCATGGGCAGCGGCAGCGAGTGGTCCA
  CGATTTTCCCAAGTACAGTGTCAAGGTTCTGCCGTGGCTCAGCCCCGAGGTCCTCCAGCAGAATCTCCAG
  GGTTATGATGCCAAGTCTGACATCTACAGTGTGGGAATCACAGCCTGTGAACTGGCCAACGGCCATGTCC
  CCTTTAAGGATATGCCTGCCACCCAGATGCTGCTAGAGAAACTGAACGGCACAGTGCCCTGCCTGTTGGA
  TACCAGCACCATCCCCGCTGAGGAGCTGACCATGAGCCCTTCGCGCTCAGTGGCCAACTCTGGCCTGAGT
  GACAGCCTGACCACCAGCACCCCCCGGCCCTCCAACGGCCCAGTGCCAGCACCCTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG228232 representing NM_001165970
  Red=Cloning site Green=Tags(s)
  
  MSFLVSKPERIRRWVSEKFIVEGLRDLELFGGKGFEDLMTVNLARYKPTGEYVTVRRINLEACSNEMVTF
  LQGELHVSKLFNHPNIVPYRATFIADNELWVVTSFMAYGSAKDLICTHFMDGMNELAIAYILQGVLKALD
  YIHHMGYVHRSVKASHILISVDGKVYLSGLRSNLSMISHGQRQRVVHDFPKYSVKVLPWLSPEVLQQNLQ
  GYDAKSDIYSVGITACELANGHVPFKDMPATQMLLEKLNGTVPCLLDTSTIPAEELTMSPSRSVANSGLS
  DSLTTSTPRPSNGPVPAPS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001165970
• ORF Size: 897 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001165970.2
• RefSeq Size: 2144 bp
• RefSeq ORF: 900 bp
• Locus ID: 92335
• UniProt ID: Q7RTN6
• Cytogenetics: 17q23.3
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: mTOR signaling pathway
• Gene Summary: The protein encoded by this gene contains a STE20-like kinase domain, but lacks several residues that are critical for catalytic activity, so it is termed a 'pseudokinase'. The protein forms a heterotrimeric complex with serine/threonine kinase 11 (STK11, also known as LKB1) and the scaffolding protein calcium binding protein 39 (CAB39, also known as MO25). The protein activates STK11 leading to the phosphorylation of both proteins and excluding STK11 from the nucleus. The protein is necessary for STK11-induced G1 cell cycle arrest. A mutation in this gene has been shown to result in polyhydramnios, megalencephaly, and symptomatic epilepsy (PMSE) syndrome. Multiple transcript variants encoding different isoforms have been found for this gene. Additional transcript variants have been described but their full-length nature is not known. [provided by RefSeq, Sep 2009]