MOSMO (NM_001164579) Human Tagged ORF Clone

CAT#: RG228103

C16orf52 (tGFP-tagged) - Human chromosome 16 open reading frame 52 (C16orf52)

Product Images

Circular map for RG228103

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MOSMO
• Synonyms: ATTHOG; BC030336; C16orf52
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG228103 representing NM_001164579
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATAAACTGACCATCATCTCAGGATGTCTCTTTCTGGCCGCCGATATCTTCGCCATCGCCAGCATCG
  CCAACCCGGACTGGATCAACACCGGGGAGTCTGCGGGAGCACTCACTGTGGGCCTCGTGCGACAGTGTCA
  AACAATCCATGGACGAGACCGGACGTGCATCCCTCCCCGGCTTCCCCCGGAGTGGGTCACCACACTGTTT
  TTTATCATCATGGGAATCATTTCATTGACTGTCACATGTGGTTTGCTGGTGGCTTCCCACTGGCGAAGAG
  AAGCTACAAAATATGCTCGATGGATAGCATTCACTGGAATGATCCTTTTCTGTATGGCTGCCCTAATATT
  TCCAATAGGATTTTACATCAATGAAGTCGGAGGTCAACCTTATAAATTACCCAACAACACAGTAGTTGGG
  TCATCATATGTACTTTTTGTCTTATCAATTTTCTTTACAATAGTAGGACTTCTATTTGCTGGCAAAGTTT
  GTTTACCAGGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG228103 representing NM_001164579
  Red=Cloning site Green=Tags(s)
  
  MDKLTIISGCLFLAADIFAIASIANPDWINTGESAGALTVGLVRQCQTIHGRDRTCIPPRLPPEWVTTLF
  FIIMGIISLTVTCGLLVASHWRREATKYARWIAFTGMILFCMAALIFPIGFYINEVGGQPYKLPNNTVVG
  SSYVLFVLSIFFTIVGLLFAGKVCLPG
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001164579
• ORF Size: 501 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001164579.2
• RefSeq Size: 4445 bp
• RefSeq ORF: 504 bp
• Locus ID: 730094
• UniProt ID: Q8NHV5
• Cytogenetics: 16p12.2
• Gene Summary: Acts as a negative regulator of hedgehog signaling probably by promoting internalization and subsequent degradation of smoothened protein (SMO) present in the ciliary membrane. Plays a role in sonic hedgehog (SHH)-induced spinal neural progenitor cells differentiation.[UniProtKB/Swiss-Prot Function]