Nuclear Factor 1 (NFIA) (NM_001145511) Human Tagged ORF Clone
CAT#: RG227920
- TrueORF®
NFIA (tGFP-tagged) - Human nuclear factor I/A (NFIA), transcript variant 3
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_001145511" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Nuclear Factor 1 |
Synonyms | BRMUTD; CTF; NF-I/A; NF1-A; NFI-A; NFI-L |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG227920 representing NM_001145511
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGAATTTCATCCTTTCATCGAAGCACTTCTGCCCCACGTCCGAGCCTTTGCCTACACATGGTTCA ACCTGCAGGCCCGAAAACGAAAATACTTCAAAAAACATGAAAAGCGTATGTCAAAAGAAGAAGAGAGAGC CGTGAAGGATGAATTGCTAAGTGAAAAACCAGAGGTCAAGCAGAAGTGGGCATCTCGACTTCTGGCAAAG TTGCGGAAAGATATCCGACCCGAATATCGAGAGGATTTTGTTCTTACAGTTACAGGGAAAAAACCTCCAT GTTGTGTTCTTTCCAACCCAGACCAGAAAGGCAAGATGCGAAGAATTGACTGCCTCCGCCAGGCAGATAA AGTCTGGAGGTTGGACCTTGTTATGGTGATTTTGTTTAAAGGTATTCCGCTGGAAAGTACTGATGGCGAG CGCCTTGTAAAGTCCCCACAATGCTCTAATCCAGGGCTCTGTGTCCAACCCCATCACATAGGGGTTTCTG TTAAGGAACTCGATTTATATTTGGCATACTTTGTGCATGCAGCAGATTCAAGTCAATCTGAAAGTCCCAG CCAGCCAAGTGACGCTGACATTAAGGACCAGCCAGAAAATGGACATTTGGGCTTCCAGGACAGTTTTGTC ACATCAGGTGTTTTTAGTGTCACTGAGCTAGTAAGAGTGTCACAGACACCAATAGCTGCAGGAACTGGCC CAAATTTTTCTCTCTCAGATTTGGAAAGTTCTTCATACTACAGCATGAGTCCAGGAGCAATGAGGAGGTC TTTACCCAGCACATCCTCTACGAGCTCCACAAAGCGCCTCAAGTCTGTGGAGGATGAAATGGACAGTCCT GGTGAGGAGCCATTTTATACAGGCCAAGGGCGCTCCCCAGGAAGTGGCAGTCAGTCAAGTGGATGGCATG AAGTGGAGCCAGGAATGCCATCTCCAACCACACTGAAGAAGTCGGAGAAGTCTGGTTTCAGCAGCCCCTC CCCTTCACAGACCTCCTCCCTGGGAACGGCGTTCACACAGCATCACCGACCTGTCATTACAGGACCCAGA GCAAGTCCGCATGCAACACCATCGACTCTTCATTTCCCGACATCACCCATTATCCAGCAGCCTGGGCCTT ACTTCTCACACCCAGCCATCCGCTATCACCCTCAGGAGACGCTGAAAGAATTTGTCCAACTTGTCTGCCC TGATGCTGGTCAGCAGGCTGGACAGGTGGGGTTCCTCAATCCCAATGGGAGCAGCCAAGGCAAGGTGCAC AACCCATTCCTTCCCACCCCAATGTTGCCACCGCCACCGCCACCACCGATGGCCAGGCCTGTGCCTCTGC CGGTGCCAGACACAAAGCCTCCAACCACGTCAACAGAAGGAGGTGCAGCCTCCCCCACGTCACCAACCTA CTCGACACCCAGCACCTCCCCCGCAAACCGATTCGTCAGTGTTGGACCACGGGATCCAAGCTTTGTAAAT ATCCCTCAACAGACACAGTCCTGGTACCTGGGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG227920 representing NM_001145511
Red=Cloning site Green=Tags(s) MDEFHPFIEALLPHVRAFAYTWFNLQARKRKYFKKHEKRMSKEEERAVKDELLSEKPEVKQKWASRLLAK LRKDIRPEYREDFVLTVTGKKPPCCVLSNPDQKGKMRRIDCLRQADKVWRLDLVMVILFKGIPLESTDGE RLVKSPQCSNPGLCVQPHHIGVSVKELDLYLAYFVHAADSSQSESPSQPSDADIKDQPENGHLGFQDSFV TSGVFSVTELVRVSQTPIAAGTGPNFSLSDLESSSYYSMSPGAMRRSLPSTSSTSSTKRLKSVEDEMDSP GEEPFYTGQGRSPGSGSQSSGWHEVEPGMPSPTTLKKSEKSGFSSPSPSQTSSLGTAFTQHHRPVITGPR ASPHATPSTLHFPTSPIIQQPGPYFSHPAIRYHPQETLKEFVQLVCPDAGQQAGQVGFLNPNGSSQGKVH NPFLPTPMLPPPPPPPMARPVPLPVPDTKPPTTSTEGGAASPTSPTYSTPSTSPANRFVSVGPRDPSFVN IPQQTQSWYLG TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001145511 |
ORF Size | 1503 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001145511.2 |
RefSeq Size | 9339 bp |
RefSeq ORF | 1506 bp |
Locus ID | 4774 |
UniProt ID | Q12857 |
Cytogenetics | 1p31.3 |
Protein Families | Transcription Factors |
Gene Summary | This gene encodes a member of the NF1 (nuclear factor 1) family of transcription factors. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC227920 | NFIA (Myc-DDK-tagged)-Human nuclear factor I/A (NFIA), transcript variant 3 |
USD 466.00 |
|
RC227920L3 | Lenti-ORF clone of NFIA (Myc-DDK-tagged)-Human nuclear factor I/A (NFIA), transcript variant 3 |
USD 766.00 |
|
RC227920L4 | Lenti-ORF clone of NFIA (mGFP-tagged)-Human nuclear factor I/A (NFIA), transcript variant 3 |
USD 766.00 |
|
SC326586 | NFIA (untagged)-Human nuclear factor I/A (NFIA), transcript variant 3, mRNA |
USD 467.00 |
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