EFCAB5 (NM_001145053) Human Tagged ORF Clone

CAT#: RG227865

EFCAB5 (tGFP-tagged) - Human EF-hand calcium binding domain 5 (EFCAB5), transcript variant 2

Product Images

Circular map for RG227865

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: EFCAB5
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG227865 representing NM_001145053
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGAAATCAAATCCCAAGAATTAAACCTGGAGGGGCAGCGAAAAATTTCACCTGGTTCAATAAAGG
  ACTCTAAAACTGAAGCCTCAGGTAATATTGCAATTAGAAAATCTGCAAAAGTGATTTTTGCTTTAGATGA
  AACTGAACTGAAATCAAAGCCAGAGCACACATGGAAGAAAAACCTTTTTGAAAGAATGGAGGCAAGAGCC
  CAAGCAATGCAGCAGAAAATAATAGATAAGGAAAATCTGAAGAAGGAACTAGAAAAAAAGGCTGAAAAAA
  AACTCCCTAGGGATAATTTGGCCAAAGAGTGGTTTAATACTGACAGCATGACACTGAATAATACTGCATA
  TTTGCTTGACAAACTTCTACCCACCTTAGTTCCTGGAGTAGAAAACATGTTAACTCAAGTAGAAAAGAAG
  AAGGTTTTGACAGAAGCTGATACTCCAAGCAAGTTTGACCCAATTAATTATTTGGGGGAATATTTAATAA
  GAAACAATCCTAATTATATCAAAGACCCAGGAATGTCTGGTTACCAGAGGTTGATGAAAGAAGTCACAGA
  AGACCTGAAGATATATGTTCCTGACACTATCTGCAACAGAGTATCCAAGATGAAGGAGAATGTTAAACAG
  AATAGAAAACAAAGAGAAAGCATAGACAAAATCAAAGTCAAGGTGGCCAACACACGGAAACAGGCTCTGC
  AGGAGCAATTCGATGAATGGATTCTAGACCCTAAAGGAATGATTCCTAAGTCAGTGATTCAGAATGTTCT
  TCAAGAATTCTTTCAAAATCCAGATTTCAAGCTTGGATCACACTGCAAACAACTGGATATTACTGACTCA
  ACAGAACCAAGATTGAACAAAATGGAATTTACAGAATACATCTCTTCACATATTAAAGACTTGAAGAGTG
  AAATGTTTGAGGAACTTCTTAAGCACCTTTGCCACTCTGCAGATGAATTTCGGGAGGTCATAAAAGCTGA
  CATGCGGAGGCAGATGTTCGCTGAACTCTTCCTACATTGTGACCACGGGAAGGTAGGGTTTTTGGATCGG
  CAGAGGACATTGGCCCTGCTGGAATTGTTCTATGACCATAGTTCACAAATGCTTAGGAGTTTACTTCGAA
  ACCCACGACAATGGCCATTCATTGAATTTGAAGAGATAAACTTGACTGAGTTGTGGGGAGACATGGATAA
  TCAGAAACACATTTATGAAGGTTTTGACAAAGTGCTCTTGGAGATGAATACATTACTTTCTGCAAATCAT
  GCTAGCAAAACCCAAAGTAAATTATTAGAAAGTCCAGATCAACCTAAACTTAACGAACAGAGAACATCAA
  CACCATCACCAAACCCGCCAGAACAGCAGAGAGGAGTAACTGCAGAACAAGGACCACAAAGAATTTCAAT
  TGAAGAACAACAACAAGGCAAAAAGCCAACTGCAGAGCAAGAACTGTACATAGAATCAGTAATAGAACCA
  GGAACACACACAGAGTCAACTCTAGAACAAGGGTCAAGTAGAAGGTTACTGACAGAACAAGAAACACACA
  GAGAGTCAACTACAGAACAAGGACAGCACAAAGGGTCAATAGAAGGACAAGGACCACGCAGAGTGTCAGT
  TTCAGAACAAGGATCAAGCAGAGAGTCAGTTGCAGAACAAGGGTCACGCAGAGAGTCTATTGCAGAACAA
  GATCGACACAAAGGGTCAGTAGCAGAACAAGGATCACGCAGAATGTCAGCTGCAGAACAGGGATCACTCA
  GAGAGTCAGTAATAGAAGAACCATACCAAAAATCAGAACAAGGACCTTATGGAGAGATAATTTCAGAAGA
  GCAAGAAGACATAGGCTCAACTTCACAATCAAGAAAAGATAGTATCTTAAAAAGTACAAAATATGGGGAA
  CCTATAACCTCTGAGTACATTGAAGTCCCTCTACAGGAAAAGAGGTCTTGGGAACAAACATATGAAGAGG
  AAATATTCCTGAGTTCTGAACTGCAAGAGGAAGTTCCAACCTTAAGCAGAAAAGATCACTTTCCAGAAAC
  TACAAAAAAGGAAGTTCAGAAAGACAAGCCCTGTGAACCCAAGTCCCAAAAAATAGAAGGAAAGTCATGG
  TCAGGTGAATTTTTTACTTGTAACTGGAAAATGAAGTATGTCACATTTGAAGATGAGGAACAGGCAAACT
  TAATCTATGGTAACTCCAGGTTCACAGATTTACACTCAATTATCAGAAATATTCAGTCTTGCAAGGAGGT
  AAAAGGCAGAACTGCCTTCAATGGAGTTTCATTCAATCTGCTCCAGTTTGTGCAACTCCTGGAGACATTT
  GTTGGTGAAGACGCTCCCTTGAGTGTCAGCGAGACTCTCACCTCCTTTTTTAAGGAGGGCTATGTTGAAA
  CAGAACAAGAGAAAATGAATGCCTTAGAACAGGTCTCACTCTGTCGCCAGGCTGGAGTGCAGTGGCGCAA
  CTGTAGCTCACTGCAGCCTCCAACTCCCGGACTCAAGCGATCATCCCATCACAGACTCCTGAGCAACTGG
  GACTACAGGGAAAGAAGGAAGTTTTTTCCTCTTGAGACCAAATTTCCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG227865 representing NM_001145053
  Red=Cloning site Green=Tags(s)
  
  MDEIKSQELNLEGQRKISPGSIKDSKTEASGNIAIRKSAKVIFALDETELKSKPEHTWKKNLFERMEARA
  QAMQQKIIDKENLKKELEKKAEKKLPRDNLAKEWFNTDSMTLNNTAYLLDKLLPTLVPGVENMLTQVEKK
  KVLTEADTPSKFDPINYLGEYLIRNNPNYIKDPGMSGYQRLMKEVTEDLKIYVPDTICNRVSKMKENVKQ
  NRKQRESIDKIKVKVANTRKQALQEQFDEWILDPKGMIPKSVIQNVLQEFFQNPDFKLGSHCKQLDITDS
  TEPRLNKMEFTEYISSHIKDLKSEMFEELLKHLCHSADEFREVIKADMRRQMFAELFLHCDHGKVGFLDR
  QRTLALLELFYDHSSQMLRSLLRNPRQWPFIEFEEINLTELWGDMDNQKHIYEGFDKVLLEMNTLLSANH
  ASKTQSKLLESPDQPKLNEQRTSTPSPNPPEQQRGVTAEQGPQRISIEEQQQGKKPTAEQELYIESVIEP
  GTHTESTLEQGSSRRLLTEQETHRESTTEQGQHKGSIEGQGPRRVSVSEQGSSRESVAEQGSRRESIAEQ
  DRHKGSVAEQGSRRMSAAEQGSLRESVIEEPYQKSEQGPYGEIISEEQEDIGSTSQSRKDSILKSTKYGE
  PITSEYIEVPLQEKRSWEQTYEEEIFLSSELQEEVPTLSRKDHFPETTKKEVQKDKPCEPKSQKIEGKSW
  SGEFFTCNWKMKYVTFEDEEQANLIYGNSRFTDLHSIIRNIQSCKEVKGRTAFNGVSFNLLQFVQLLETF
  VGEDAPLSVSETLTSFFKEGYVETEQEKMNALEQVSLCRQAGVQWRNCSSLQPPTPGLKRSSHHRLLSNW
  DYRERRKFFPLETKFP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001145053
• ORF Size: 2568 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001145053.1, NP_001138525.1
• RefSeq Size: 3762 bp
• RefSeq ORF: 2571 bp
• Locus ID: 374786
• UniProt ID: A4FU69
• Cytogenetics: 17q11.2