TMEM25 (NM_001144035) Human Tagged ORF Clone
CAT#: RG227827
- TrueORF®
TMEM25 (tGFP-tagged) - Human transmembrane protein 25 (TMEM25), transcript variant 6
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_001144035" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | TMEM25 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG227827 representing NM_001144035
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCTGCCTCCAGGCCCAGCCGCCCTCCGGCACACACTGCTGCTCCTGCCAGCCCTTCTGAGCTCAG GTTGGGGGGAGTTGGAGCCACAAATAGATGGTCAGACCTGGGCTGAGCGGGCACTTCGGGAGAATGAACG CCACGCCTTCACCTGCCGGGTGGCAGGGGGGCCTGGCACCCCCAGATTGGCCTGGTATCTGGATGGACAG CTGCAGGAGGCCAGCACCTCAAGACTGCTGAGCGTGGGAGGGGAGGCCTTCTCTGGAGGCACCAGCACCT TCACTGTCACTGCCCATCGGGCCCAGCATGAGCTCAACTGCTCTCTGCAGGACCCCAGAAGTGGCCGATC AGCCAACGCCTCTGTCATCCTTAATGTGCAATTCAAGCCAGAGATTGCCCAAGTCGGCGCCAAGTACCAG GAAGCTCAGGGCCCAGGCCTCCTGGTTGTCCTGTTTGCCCTGGTGCGTGCCAACCCGCCGGCCAATGTCA CCTGGATCGACCAGGATGGGCCAGTGACTGTCAACACCTCTGACTTCCTGGTGCTGGATGCGCAGAACTA CCCCTGGCTCACCAACCACACGGTGCAGCTGCAGCTCCGCAGCCTGGCACACAACCTCTCGGTGGTGGCC ACCAATGACGTGGGTGTCACCAGTGCGTCGCTTCCAGCCCCAGGCCCCTCCCGGCACCCATCTCTGATAT CAAGTGACTCCAACAACCTAAAACTCAACAACGTGCGCCTGCCACGGGAGAACATGTCCCTCCCGTCCAA CCTTCAGCTCAATGACCTCACTCCAGATTCCAGAGCAGTGAAACCAGCAGACCGGCAGATGGCTCAGAAC AACAGCCGGCCAGAGCTTCTGGACCCGGAGCCCGGCGGCCTCCTCACCAGCCGAGGTTTCATCCGCCTCC CAGTGCTGGGCTATATCTATCGAGTGTCCAGCGTGAGCAGTGATGAGATCTGGCTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG227827 representing NM_001144035
Red=Cloning site Green=Tags(s) MALPPGPAALRHTLLLLPALLSSGWGELEPQIDGQTWAERALRENERHAFTCRVAGGPGTPRLAWYLDGQ LQEASTSRLLSVGGEAFSGGTSTFTVTAHRAQHELNCSLQDPRSGRSANASVILNVQFKPEIAQVGAKYQ EAQGPGLLVVLFALVRANPPANVTWIDQDGPVTVNTSDFLVLDAQNYPWLTNHTVQLQLRSLAHNLSVVA TNDVGVTSASLPAPGPSRHPSLISSDSNNLKLNNVRLPRENMSLPSNLQLNDLTPDSRAVKPADRQMAQN NSRPELLDPEPGGLLTSRGFIRLPVLGYIYRVSSVSSDEIWL TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001144035 |
ORF Size | 966 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001144035.1, NP_001137507.1 |
RefSeq Size | 2416 bp |
RefSeq ORF | 969 bp |
Locus ID | 84866 |
UniProt ID | Q86YD3 |
Cytogenetics | 11q23.3 |
Protein Families | Transmembrane |
Gene Summary | In neurons, modulates the degradation of NMDA receptor GRIN2B subunit. Plays a role in the regulation of neuronal excitability.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC227827 | TMEM25 (Myc-DDK-tagged)-Human transmembrane protein 25 (TMEM25), transcript variant 6 |
USD 330.00 |
|
RC227827L3 | Lenti-ORF clone of TMEM25 (Myc-DDK-tagged)-Human transmembrane protein 25 (TMEM25), transcript variant 6 |
USD 630.00 |
|
RC227827L4 | Lenti-ORF clone of TMEM25 (mGFP-tagged)-Human transmembrane protein 25 (TMEM25), transcript variant 6 |
USD 630.00 |
|
SC324943 | TMEM25 (untagged)-Human transmembrane protein 25 (TMEM25), transcript variant 6 |
USD 330.00 |
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