LANPL (ANP32E) (NM_001136478) Human Tagged ORF Clone
CAT#: RG227678
- TrueORF®
ANP32E (tGFP-tagged) - Human acidic (leucine-rich) nuclear phosphoprotein 32 family, member E (ANP32E), transcript variant 2
ORF Plasmid: DDK
"NM_001136478" in other vectors (2)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | LANPL |
Synonyms | LANP-L; LANPL |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG227678 representing NM_001136478
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGATGAAGAAGAAGATTAACCTGGAGTTAAGGAACAGATCCCCGGAGGAGGTGACAGAGTTAGTCC TTGATAATTGCCTGTGTGTCAATGGGGAAATTGAAGGCCTGAATGATACTTTCAAAGAACTAGAATTTCT GAGTATGGCTAATGTGGAACTAAGTTCGCTGGCCCGGCTTCCCAGCTTAAATAAACTTCGAAAACAAAAT CTTAAAAATTTGAAAAGTCTTGACCTGTTTAACTGTGAGATCACAAACCTGGAAGATTATAGAGAAAGTA TTTTTGAACTACTGCAGCAAATCACATACTTAGATGGATTTGATCAGGAGGATAATGAAGCGCCGGACTC TGAAGAGGAGGATGATGAGGATGGCGATGAAGATGATGAAGAGGAAGAGGAAAATGAAGCTGGTCCACCG GAAGGATATGAGGAAGAGGAGGAGGAAGAGGAAGAGGAGGATGAGGATGAGGATGAAGATGAAGATGAAG CAGGTTCAGAGTTGGGAGAGGGAGAAGAGGAAGTGGGCCTCTCATACTTAATGAAAGAAGAAATTCAGGA TGAAGAAGATGATGATGACTATGTTGAAGAAGGGGAAGAAGAGGAAGAAGAGGAAGAAGGAGGTCTTCGA GGGGAGAAGAGGAAACGAGATGCTGAAGACGATGGAGAGGAAGAAGATGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG227678 representing NM_001136478
Red=Cloning site Green=Tags(s) MEMKKKINLELRNRSPEEVTELVLDNCLCVNGEIEGLNDTFKELEFLSMANVELSSLARLPSLNKLRKQN LKNLKSLDLFNCEITNLEDYRESIFELLQQITYLDGFDQEDNEAPDSEEEDDEDGDEDDEEEEENEAGPP EGYEEEEEEEEEEDEDEDEDEDEAGSELGEGEEEVGLSYLMKEEIQDEEDDDDYVEEGEEEEEEEEGGLR GEKRKRDAEDDGEEEDD TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_001136478 |
ORF Size | 681 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001136478.4 |
RefSeq Size | 3347 bp |
RefSeq ORF | 684 bp |
Locus ID | 81611 |
UniProt ID | Q9BTT0 |
Cytogenetics | 1q21.2 |
Protein Families | Druggable Genome |
Gene Summary | Histone chaperone that specifically mediates the genome-wide removal of histone H2A.Z/H2AFZ from the nucleosome: removes H2A.Z/H2AFZ from its normal sites of deposition, especially from enhancer and insulator regions. Not involved in deposition of H2A.Z/H2AFZ in the nucleosome. May stabilize the evicted H2A.Z/H2AFZ-H2B dimer, thus shifting the equilibrium towards dissociation and the off-chromatin state (PubMed:24463511). Inhibits activity of protein phosphatase 2A (PP2A). Does not inhibit protein phosphatase 1. May play a role in cerebellar development and synaptogenesis.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC227678 | ANP32E (Myc-DDK-tagged)-Human acidic (leucine-rich) nuclear phosphoprotein 32 family, member E (ANP32E), transcript variant 2 |
USD 330.00 |
|
SC325633 | ANP32E (untagged)-Human acidic (leucine-rich) nuclear phosphoprotein 32 family, member E (ANP32E), transcript variant 2 |
USD 330.00 |
{0} Product Review(s)
Be the first one to submit a review