ZNF541 (NM_001101419) Human Tagged ORF Clone

CAT#: RG226738

ZNF541 (tGFP-tagged) - Human zinc finger protein 541 (ZNF541)

Product Images

Circular map for RG226738

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ZNF541
• Synonyms: DKFZp434I1930; MGC119518; MGC119519; MGC119521
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG226738 representing NM_001101419
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCAGTACAGCCTTGGAGACGAGGGTGCCCTCCCATCAGAAATGCACCTCCCTTCATTTTCAGAGA
  GCCAAGGGCTCAACTGCAGCGACACCCTCAACCGGGATTTGGGTCCCAACACGCGAGGCTTTCTTTATGC
  TGGCCTGAGTGGTCTGGACCCGGACCCCAGCCTCCCAACGCCTGACATGTCCAGCGAGGTGCTGGAGGAC
  AACTTAGACACCTTGTCCCTGTACTCCGGGAAGGACAGTGATTCTGTGAAGCTGCTGGAGGAGTACGCAG
  ATTCGGAGTCTCAGGCATCCTTACAAGACCTGGGGCTAGGTGTGCTTAAGGCTAAAGAGGCTGACGAAGG
  AGGAAGGGCCACCTCGGGGAGTGCCAGGAAAGGAAAGCGGCAGCACAGTTCCCCTCAAAACCCACTTCTG
  GACTGCAGCCTGTGCGGGAAGGTGTTCAGCAGCGCCAGTTCTCTGAGCAAGCACTACCTGACACACAGCC
  AGGAAAGGAAGCACGTCTGCAAAATCTGCAGCAAGGCCTTTAAGCGCCAGGACCACCTGACCGGGCACAT
  GCTCACCCACCAGAAGACAAAGCCCTTCGTGTGCATCGAGCAGGGCTGCAGCAAGAGCTACTGCGACTAC
  CGCTCTCTGCGCCGGCACTACGAGGTCCATCACGGCCTGTGCATCCTGAAGGAAGCCCCCCCGGAGGAAG
  AGGCCTGCGGGGACTCCCCCCACGCCCACGAGTCGGCCGGCCAGCCGCCCCCCAGCAGCCTGCGGTCCCT
  GGTGCCCCCAGAGGCCAGGTCCCCCGGCTCCCTCCTGCCCCACCGGGACCTCCTGCGCCGCATCGTGAGT
  AGCATCGTCCACCAGAAGACCCCTTCTCCTGGCCCAGCCCCGGCGGGGGCTTCAGACAGCGAAGGGAGGA
  ACACTGCCTGTCCCTGCCCCGCCTCATCGGGGTCCTCGTCCTGCACCCCAGCCGGCCCCCACGCGGCCCC
  AGCAGCGCTGGACACCGAGCTTCCCGAGGAGCCTTGCCTCCCACAGAAAGAGCCGGCCACTGACGTGTTC
  ACAGCCCCTAATTCCAGGGCCGCCGAGAACGGCGCCCCCGACCCGCCAGAGCCGGAGCCAGATACCGCGC
  TGCTCCAGGCCCGGTCCACCGCGGAGTGCTGGCCCGAAGGCGGCTCCGTGCCTGCCTGCCTGCCTCTCTT
  CCGAGGCCAGACGGTCCCTGCCAGTTCCCAGCCATCGAGCCACAGCTTCCAGTGGCTCCGGAACCTGCCG
  GGCTGTCCCAAAAGCAAAGGCAACAACGTGTTTGTTGTCCACAAGCCCTCGGCCGTGCCCTCGCGGGAGG
  GCTCCGAGTCTGGCCCGGGACCCAGCAGCGGAAGCCCCTCGGAGGAGTCCCCGCCCGGCCCCGGCGGTGG
  CCTGGAGGATGCTCTGCCCTTCCCTGCCGCGCTCCTCAGGGTCCCCGCGGAGGCCCCGAGCGACCCCAGG
  TCGGCCAGCGGGGAAGATGACCCCTGCGCCCCCAAGAAGGTCAAGGTCGACTGCGACTCCTTCCTGTGCC
  AGAACCCCGGGGAGCCCGGCCTCCAGGAGGCCCAGAAGGCAGGCGGGCTCCCTGCGGATGCCTCGCCGCT
  CTTCCGCCAGCTCTTCCTCAAGTCGCAGGAACCTCTTGTGAGCCACGAGCAGATGCAGGTGTTCCAGATG
  ATCACCAAGTCCCAGCGGATCTTCTCCCATGCCCAGGTGGCAGCAGTCTCCTCCCAGCTCCCTGCGCCCG
  AGGGCAAACCAGCCGCCCTGAGGCCGCTGCAGGGGCCGTGGCCGCAGCAGCCCCCACCACTGGCTCCTGC
  TGTGGACTCTCTCCACGCCGGCCCTGGAAACCCCGAGGCAGAGGGCTCCCCAGCCCGCAGGAGAAAAACC
  ACACCCGGGGTTCCCAGAGAGGCCTCCCCCGGCAGCACGAGACGAGACGCAAAGGGGGGACTGAAAGTGG
  CCGCGGTTCCAACGCCCCTTGCAGCACCGTCTCTGGACCCTTCCAGGAATCCAGACATCTCTTCTCTGGC
  CAAGCAGCTGCGATCCTCTAAAGGGACCTTGGACCTGGAGGACATCTTCCCCTCCACAGGCCAACGGCAG
  ACCCAGTTAGGTGGGGAGGAGCCACCAGGAGCCTCGCTGCCAGGGAAGCAGGCCCCAGCCGAGAATGGCG
  CGGCTTCAAGGATCACAAAAGGTGAAAAGGGCCCAGCCTGCTCCCGGGGTGGAGGCTACCGGCTCTTGGG
  CAACCCCAGGGCCCCGCGATTCTCCGGCTTCCGGAAAGAGAAGGCGAAGATGGATATGTGCTGTGCGGCT
  TCTCCGAGCCAGGTAGCCATGGCCTCCTTCTCATCGGCCGGGCCCCCGGCAGATCCCTCCAAGTCCAAGC
  TGACAATATTCAGCAGAATCCAGGGTGGAAACATCTACAGGCTCCCCCATCCGGTGAAGGAAGAGAATGT
  GGCAGGCAGAGGTAACCAGCAAAACGGCAGTCCCACAGACTGGACGAAGCCCAGGAGCACTTTTGTCTGC
  AAGAACTGCAGCCAGATGTTTTATACGGAGAAAGGGCTGAGCAGCCACATGTGTTTTCACAGCGACCAGT
  GGCCGTCACCTCGAGGGAAGCAGGAACCGCAGGTGTTTGGCACAGAGTTTTGCAAGCCGCTAAGACAGGT
  GCTGAGGCCAGAAGGGGACAGGCATAGTCCCCCAGGAACCAAGAAGCCCTTGGACCCCACAGCTGCAGCC
  CCTTTGGTGGTCCCCCAATCGATCCCCGTGGTTCCAGTGACCCGACACATAGGGAGCATGGCCATGATGG
  AGTGGTGCAGTAGGTGGCTTCAGGCGACCACCTGTGGTTCTAAACTTCTTCAGGGACAAGAGAAAGACGG
  GGAGGAGCGAGACAGCAAGGAGAGCAGCCAGCAGAGAAAGCGGAAGAAGCGGCCCCCACCCTCCACGGCT
  GGGGAGCCTGGCCCTGCAGGATGCCACCAGAGCCGCCTGCGGTCACCCATGTTCCTGGTGGACTGCCTCC
  TGAAGGGCTTATTCCAGTGCTCCCCCTACACACCACCCCCAATGCTCAGCCCCATCCGGGAGGGCTCTGG
  GGTGTACTTCAACACCCTCTGTTCCACGTCCACTCAGGCCAGCCCTGACCAGCTCATCAGCTCCATGCTC
  GATCAAGTGGATGGGTCCTTTGGCATCTGTGTGGTGAAGGATGACACCAAAATCAGCATTGAGCCACATA
  TCAATATAGGAAGCCGGTTTCAGGCAGAGATCCCGGAACTCCAGGAGAGATCCCTGGCTGGAACTGACGA
  GCATGTAGCTTCTCTGGTCTGGAAGCCATGGGGAGATATGATGATCAGCTCAGAGACACAGGATAGAGTG
  ACCGAGCTCTGCAATGTGGCATGCTCCAGCGTGATGCCAGGAGGGGGCACCAACCTGGAGCTCGCTCTGC
  ACTGCCTGCACGAGGCTCAGGGCAACGTTCAGGTCGCCCTGGAGACTCTTCTGCTCCGAGGGCCCCACAA
  GCCACGGACACACCTGCTCGCTGACTATCGCTACACAGGTTCAGACGTCTGGACCCCTATAGAGAAGAGG
  CTTTTTAAGAAGGCGTTCTATGCCCACAAGAAGGACTTCTACTTGATACACAAGATGATCCAGACAAAGA
  CGGTAGCTCAGTGCGTTGAGTATTATTACATCTGGAAAAAAATGATCAAGTTTGACTGTGGCCGAGCCCC
  AGGGCTAGAAAAGAGGGTCAAGAGAGAGCCGGAGGAAGTGGAAAGGACAGAGGAAAAGGTCCCATGCAGC
  CCTCGGGAGAGACCCAGCCACCATCCAACTCCCAAGTTAAAGACCAAGAGTTATAGGAGGGAGTCCATCC
  TCAGCTCCAGCCCAAATGCAGGCTCCAAGCGGACCCCCGAGCTGTTGGGAAGTGCAGAGAGCCAGGGCAT
  TTTTCCATGCAGAGAGTGTGAAAGGGTGTTTGACAAGATCAAGAGTCGAAATGCCCATATGAAGCGGCAC
  CGCCTTCAGGACCACGTGGAGCCCATCATCAGGGTGAAGTGGCCAGTGAAGCCCTTCCAGCTAAAGGAAG
  AGGAGCTGGGAGCTGACATCGGCCCCCTGCAGTGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG226738 representing NM_001101419
  Red=Cloning site Green=Tags(s)
  
  MDQYSLGDEGALPSEMHLPSFSESQGLNCSDTLNRDLGPNTRGFLYAGLSGLDPDPSLPTPDMSSEVLED
  NLDTLSLYSGKDSDSVKLLEEYADSESQASLQDLGLGVLKAKEADEGGRATSGSARKGKRQHSSPQNPLL
  DCSLCGKVFSSASSLSKHYLTHSQERKHVCKICSKAFKRQDHLTGHMLTHQKTKPFVCIEQGCSKSYCDY
  RSLRRHYEVHHGLCILKEAPPEEEACGDSPHAHESAGQPPPSSLRSLVPPEARSPGSLLPHRDLLRRIVS
  SIVHQKTPSPGPAPAGASDSEGRNTACPCPASSGSSSCTPAGPHAAPAALDTELPEEPCLPQKEPATDVF
  TAPNSRAAENGAPDPPEPEPDTALLQARSTAECWPEGGSVPACLPLFRGQTVPASSQPSSHSFQWLRNLP
  GCPKSKGNNVFVVHKPSAVPSREGSESGPGPSSGSPSEESPPGPGGGLEDALPFPAALLRVPAEAPSDPR
  SASGEDDPCAPKKVKVDCDSFLCQNPGEPGLQEAQKAGGLPADASPLFRQLFLKSQEPLVSHEQMQVFQM
  ITKSQRIFSHAQVAAVSSQLPAPEGKPAALRPLQGPWPQQPPPLAPAVDSLHAGPGNPEAEGSPARRRKT
  TPGVPREASPGSTRRDAKGGLKVAAVPTPLAAPSLDPSRNPDISSLAKQLRSSKGTLDLEDIFPSTGQRQ
  TQLGGEEPPGASLPGKQAPAENGAASRITKGEKGPACSRGGGYRLLGNPRAPRFSGFRKEKAKMDMCCAA
  SPSQVAMASFSSAGPPADPSKSKLTIFSRIQGGNIYRLPHPVKEENVAGRGNQQNGSPTDWTKPRSTFVC
  KNCSQMFYTEKGLSSHMCFHSDQWPSPRGKQEPQVFGTEFCKPLRQVLRPEGDRHSPPGTKKPLDPTAAA
  PLVVPQSIPVVPVTRHIGSMAMMEWCSRWLQATTCGSKLLQGQEKDGEERDSKESSQQRKRKKRPPPSTA
  GEPGPAGCHQSRLRSPMFLVDCLLKGLFQCSPYTPPPMLSPIREGSGVYFNTLCSTSTQASPDQLISSML
  DQVDGSFGICVVKDDTKISIEPHINIGSRFQAEIPELQERSLAGTDEHVASLVWKPWGDMMISSETQDRV
  TELCNVACSSVMPGGGTNLELALHCLHEAQGNVQVALETLLLRGPHKPRTHLLADYRYTGSDVWTPIEKR
  LFKKAFYAHKKDFYLIHKMIQTKTVAQCVEYYYIWKKMIKFDCGRAPGLEKRVKREPEEVERTEEKVPCS
  PRERPSHHPTPKLKTKSYRRESILSSSPNAGSKRTPELLGSAESQGIFPCRECERVFDKIKSRNAHMKRH
  RLQDHVEPIIRVKWPVKPFQLKEEELGADIGPLQW
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001101419
• ORF Size: 4095 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001101419.1, NP_001094889.1
• RefSeq Size: 4632 bp
• RefSeq ORF: 4097 bp
• Locus ID: 84215
• Cytogenetics: 19q13.33
• Gene Summary: Component of some chromatin remodeling multiprotein complex that plays a role during spermatogenesis.[UniProtKB/Swiss-Prot Function]