NDOR1 (NM_001144026) Human Tagged ORF Clone

CAT#: RG226636

NDOR1 (tGFP-tagged) - Human NADPH dependent diflavin oxidoreductase 1 (NDOR1), transcript variant 1

Product Images

Circular map for RG226636

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: NDOR1
• Synonyms: bA350O14.9; CIAE1; NR1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG226636 representing NM_001144026
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGAGCCCGCAGCTTCTGGTGCTCTTCGGCAGCCAGACAGGCACGGCTCAGGATGTGTCGGAGAGAC
  TGGGTCGCGAGGCCCGGCGCCGGCGGCTTGGCTGCCGGGTGCAGGCCCTGGACTCCTACCCGGTGGTGAA
  TCTGATTAACGAGCCCCTGGTGATATTTGTTTGTGCAACTACAGGCCAAGGAGACCCCCCTGACAACATG
  AAGAACTTCTGGAGGTTTATATTCCGGAAGAACCTGCCCTCCACTGCCCTCTGTCAGATGGACTTTGCCG
  TCCTGGGCCTCGGGGACTCCTCATACGCCAAGTTCAACTTCGTGGCCAAGAAGCTGCACCGACGGCTACT
  GCAGCTTGGGGGCAGCGCCCTCCTGCCCGTGTGCCTGGGCGATGACCAGCATGAGCTGGGGCCCGACGCT
  GCTGTGGACCCCTGGCTGCGAGACTTGTGGGACAGGGTTCTGGGGCTGTACCCGCCGCCTCCGGGCCTCA
  CTGAGATCCCTCCCGGAGTCCCCCTGCCCTCCAAGTTCACCCTGCTGTTCCTCCAAGAGGCACCCAGCAC
  GGGCTCTGAGGGGCAGCGGGTAGCTCACCCCGGCTCTCAGGAGCCCCCGTCAGAGTCGAAGCCCTTCCTA
  GCACCCATGATCTCCAACCAGAGAGTCACCGGCCCCTCCCACTTCCAGGACGTTCGGCTGATTGAGTTTG
  ACATCTTGGGCTCTGGCATCAGCTTTGCTGCTGGTGATGTGGTGCTGATTCAGCCCTCCAACTCGGCTGC
  CCATGTCCAGCGGTTCTGCCAGGTGCTGGGCCTGGACCCTGACCAGCTCTTCATGCTGCAGCCGCGGGAG
  CCAGATGTCTCCTCCCCCACGAGGCTGCCCCAGCCCTGCTCCATGCGGCACCTCGTGTCCCACTACCTGG
  ACATCGCCAGCGTGCCTCGCCGCTCCTTCTTCGAACTCCTGGCCTGTCTATCCCTCCATGAGCTGGAGCG
  GGAGAAGCTGCTGGAGTTCAGTTCTGCCCAAGGCCAGGAGGAGCTCTTTGAATACTGCAACCGGCCCCGC
  AGGACCATCCTGGAGGTGCTCTGTGACTTCCCGCACACAGCTGCCGCCATCCCTCCCGACTACCTGTTGG
  ACCTCATCCCCGTTATCCGGCCGAGGGCCTTCTCCATCGCCTCCTCGCTGCTGACTCACCCCTCACGGCT
  GCAGATCCTCGTGGCTGTAGTGCAGTTCCAGACTCGCCTCAAGGAGCCCCGCCGGGGCCTCTGCTCCTCC
  TGGCTGGCATCCCTGGACCCTGGGCAAGGACCTGTCCGGGTGCCCCTCTGGGTGCGGCCTGGGAGTCTGG
  CCTTCCCAGAGACACCAGACACACCTGTGATCATGGTGGGGCCTGGCACTGGGGTAGCCCCCTTCCGAGC
  AGCCATCCAGGAGCGTGTGGCCCAGGGCCAGACTGGAAACTTCTTGTTTTTTGGCTGCCGCTGGCGGGAC
  CAAGACTTCTACTGGGAGGCTGAGTGGCAGGAGCTGGAGAAGCGGGACTGTCTGACCCTCATCCCTGCCT
  TCTCCCGGGAACAGCCCCCAGCCCTGTTCTCTGCCCTACAGGAGCAGAAAGTATATGTGCAGCACCGGCT
  CCGGGAGCTGGGGTCGCTTGTGTGGGAACTGCTGGACCGCCAGGGTGCATACTTCTACCTGGCAGGCAAC
  GCCAAGTCCATGCCAGCGGACGTCTCGGAAGCCCTGATGTCCATCTTCCAGGAGGAGGGTGGACTCTGCA
  GCCCGGACGCAGCCGCGTATCTAGCCAGGCTCCAGCAGACACGGCGCTTCCAGACAGAGACGTGGGCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG226636 representing NM_001144026
  Red=Cloning site Green=Tags(s)
  
  MPSPQLLVLFGSQTGTAQDVSERLGREARRRRLGCRVQALDSYPVVNLINEPLVIFVCATTGQGDPPDNM
  KNFWRFIFRKNLPSTALCQMDFAVLGLGDSSYAKFNFVAKKLHRRLLQLGGSALLPVCLGDDQHELGPDA
  AVDPWLRDLWDRVLGLYPPPPGLTEIPPGVPLPSKFTLLFLQEAPSTGSEGQRVAHPGSQEPPSESKPFL
  APMISNQRVTGPSHFQDVRLIEFDILGSGISFAAGDVVLIQPSNSAAHVQRFCQVLGLDPDQLFMLQPRE
  PDVSSPTRLPQPCSMRHLVSHYLDIASVPRRSFFELLACLSLHELEREKLLEFSSAQGQEELFEYCNRPR
  RTILEVLCDFPHTAAAIPPDYLLDLIPVIRPRAFSIASSLLTHPSRLQILVAVVQFQTRLKEPRRGLCSS
  WLASLDPGQGPVRVPLWVRPGSLAFPETPDTPVIMVGPGTGVAPFRAAIQERVAQGQTGNFLFFGCRWRD
  QDFYWEAEWQELEKRDCLTLIPAFSREQPPALFSALQEQKVYVQHRLRELGSLVWELLDRQGAYFYLAGN
  AKSMPADVSEALMSIFQEEGGLCSPDAAAYLARLQQTRRFQTETWA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001144026
• ORF Size: 1818 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001144026.3
• RefSeq Size: 4877 bp
• RefSeq ORF: 1821 bp
• Locus ID: 27158
• UniProt ID: Q9UHB4
• Cytogenetics: 9q34.3
• Gene Summary: This gene encodes an NADPH-dependent diflavin reductase that contains both flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD) binding domains. The encoded protein catalyzes the transfer of electrons from NADPH through FAD and FMN cofactors to potential redox partners. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2012]