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URB1 (NM_014825) Human Tagged ORF Clone
CAT#: RG226482
- TrueORF®
URB1 (tGFP-tagged) - Human URB1 ribosome biogenesis 1 homolog (S. cerevisiae) (URB1)
ORF Plasmid: DDK
"NM_014825" in other vectors (2)
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Other products for "URB1"
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Symbol | URB1 |
| Synonyms | C21orf108; NPA1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG226482 representing NM_014825
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGTCCCCAAGAGGAAGGCCTCGGGCGGCCAGGACGGCGCGGCTTCCTCCGCGGGTGCAGCCAAGC GCGCCCGCAAAGAAGAGCTCACGGGCGTGCGGTTCAAGGCTCAGCTGAAGGACCCGCAGGGCCCCGGGCC AGGCTTGGAAGCGTTTGTGTCTGCTGCCAAGAAGCTACCACGAGAAGATGTGTATGATGTTGTGGAAGGG TATATAAAGATTTCTGTTGAGTGTGTCGAAATTTTCCAGCTCCTAAGTGGAGAGAAACGACCTGAAAGTG AAACGATGTTAATATTTCAAGTTTTCGAGGCCATATTATTGCGGACAGCAAGTGATCTTTCACATTTCCA TGTTGTGGGAACCAACATTGTGAAAAAGCTGATGAACAACCACATGAAGCTCATCTGTGAGTCCCTGTAT GCCTCAGGTTACAGGTTGGCTCGCGCCTGCCTGAGCCTGATGACCGCCATGGTGACCCAGGGTCCGGAAG CTGCCAGGGACGTCTGCAGCCATTTTGATTTGAATAAAAAGACCCTGTACACCCTGGTGACCAAGAGGGA TTCAAAGGGAGTGTACGACGTTCGGCAGGCCTACGTTCAGTTTGCTCTCTCCTTTTTAATTGCGGGTGAT GACAGCACTATAGTGCAGGTGTTGGAAGTGAAAGAATTTATTCCTTGCATTTTTAGCTCAGGGATAAAGG AAGATAGGATCTCTACCATCAATATTTTATTATCCACACTGAAAACAAAGGTAGTTCACAATAAAAATAT CACAAAAACCCAGAAGGTGCGTTTCTTTACGGGGCAGTTATTGAACCACATAGCATCGCTGTACAACTGG AATGGGATTACCGATGTGAACCCAGAAAATGTCAAGGTTTCTGCCGAAGAAGCAGGGAAAACCATGGTGC GGGAGCTTGTTCATAACTTCCTGATGGATCTTTGCTGTTCACTCAAGCATGGAATTAATTTTTACGATGC ATCTTTGGGTACCTTTGGCAGAGGCGGAAACCTGACACTCTTGCACTTCTTGCTGGGTTTGAAAACTGCA GCTGATGATGATCTGGTGGCTGACCTTGTGGTAAACATCCTCAAAGTGTGCCCGGACTTACTGAACAAAT ACTTCAAGGAAGTAACGTTTTCCTTTATCCCACGAGCGAAGTCTACTTGGTTAAATAATATCAAACTACT AAACAAGATCTATGAGGCTCAGCCGGAGATTTCCCGGGCATTTCAGACCAGAGAGTTTATACCCTTGCCT CGGCTCCTGGCAATGGTGATGGTGACCACCGTGCCCCTGGTGTGCAATAAGAGCATGTTCACCCAAGCAT TAAACTTGGACAGCACCTCAGTGAGGCACACAGCCTTATCCCTTATTTCTGTCATTTTGAAGAGGGCATT AAAAACTGTTGACCACTGCCTGAATAAAGAGGTGTGGCAGGAATCAGGCGTGTACACAGCTGTGATGATG GAAGAATTCGTGCAGCTCTTCAGAGAAGCCCTGAGCAAGATTTTGCCAGACCTGAACACTGTCGTGTGGG TCTGGCAGTCACTTAAAAAGCAAGAGACCAAACAGGATGACAAGAAAGGGCAGAAAAGGAGCGATGGGCC CCCGGCTGCCTGCGATGCTCACCAGTGCGATGATGCAGAAACCATTCTTTTGAAAGCTGTTTTGCTCCAG GTCATATGCCTCTACCAGAAGGTGGTCCCCCACGTGGTCATGCAGTACAACTTTGACTTCAGCAAGCTCC TGAAAGGTGTCATCTCTGAGCAGGGCCTTCGAGAGGAGGTGCCTCCCATTCTGCAGCACCACATGCTGAA GGTGGCCCTGGAGCTGCCGGCCAGCAAGTTTCTGTGGTTAAAGGCACAGGAAGGCCCAGATGCAGAAATT ATTGGAGGTGAACGATCAGTATTTTACTTACTAATGAAAATGTTTGTGACCAGTAGCCATTTACAACTGA AGTCATTGACCAAACTTCTGATCATGAAGATTCTGCGGGACACGGGGGTGTTTGAGCACACCTGGAAGGA GCTGGAGCTCTGGCTGGAGCATTTAGAGAACACCATGGAAGAGGACAAAGAGACTGTGATTCAGTTTCTG GAACGCATTTTATTGACATTGGTGGCGAATCCCTATTCATACACAGACAAAGCATCTGACTTTGTCCAAG AAGCAAGCATGCTGCAGGCCACTATGACGAAGCAAGAAGCCGATGACATGAGCATTCCCATCTCCCACAT TGACGATGTTCTCGACATGGTGGATGTCCTGGTGGAGGGCAGTGAAGGCTTGGATGAGGAAATAGGGTTC ACGTTGAGTGAAGACATGATCCTGCTCACGTTCCCATTCAGTGCGGTAGTCCCTGCGGCCCTGGAAGCCA GGAATAAGTTGCTCCTTGGGACAGGCAATGAAGCAGCGGAAAACGTTGTGACATATCTGACGGCAGTGCT GACTGACCTCCTCCACACCCAGAGGGACCCCCTGGCTCTGTGTCTCCTGCTCCAGGCATATGATAAGCTT GAGCCTCCATGCCTGGTGCCTTGCTGCCAGCAGCTCTCACGGTTTAACAGATACTACAGCCTCTGGATCC CGGAGCAAGCCCGAGAGGCCTGGCTGCTGCAGGCACAGGGCAGCCCCTCGCCCCCTGCCCTTCCCTTGGC CTCGTCCTTCACAGCCCTGCTGCAGGCAGCCTACGAGAGCCAAGCGCTTCGGGACGAGCACATCCAGGTG CAGCTGCAGGCCACCATGCCACACCTGTCCATGCAGCAGGTCCTGCTCGCGGCCAAGCAGGTGTTGCTCT ACCTGCGGAGCACTGTGGAGAACTTCGGCCAGCTGGGCAGAAGTGTGGGCCCGCCCCTCCTGCAGCTCTT CCTGGATCTCCTCAGGCGCCTGGTTGTCCACTGTGAGCAGCTGGATGCCCAGAACCAGCAGAGATGCGAG GCCGCCCGGGCCGAAGCCGACCTCTTCCTGGACATGGAGTCCGTGGCCTCGCTGGAGTTGGCCAATGATC AGACGCTGGAGGAGGTGCTTGTGGCCATCCTCAGGCACCCCACCCTGGAGGGCTGGTTCCTGGCCCTGGA GCAGCAGGCCCTCCCGCCGCACACGCTCAGCCCTGTCCTCGTGAAGCTCCTGGCCACCCACTTTAGTGCA GGGGTCCTTCAGCTGCTGGCAGCAAGTGCCCCGATCCTCCAGAACATTGGGCAGCTGGGCCTTCTGGCCA GGTACTCAGAGGCCATCACCCAGAGTGTGCTGAAGGAACTTCAGAACAGGAGGGCGGGCCCGGCCACATC ACCACCAAAGACCCCGCCGCAGCTGGAGGCCCTGCAGGAGCTGCATCCATACATGGAGGGTGCCCAGCTC CGTGAGGTCACCCTGGCCTTGCTGAGCCTGCCTGAGACACACCTGGTGACCCAGCAACCCACGAAATCCC CCGGGAAGGAAAGACACCTGAATGCTCTTGGAAAGACCCTGGTGCAGCTGCTGACCTGCAGCCCCCAGGA TCAGCTGCAGAGTGGTGAGCTCCTGTGGTCCTCCGAGTATGTGAGAGGCCTGGGGGCTCTGCTGCCCACG CTAGCAGTGGACGAGCTGGACACAGTGCTCCTCCACACTCTGCAGAGAGACCCTGTGCTGGCCCCCGCAG TCGGGGCTGATCTGCTTGACTACTGCCTGGCCCGGCGCACACAGGCGGCCCTCAGCATCGCTGCCCTGCT CCTCCAGGAGAGCTGCACCCACTTGCTGTGGTTCGAGCAGTGGTGCCTGCAGGCTGGCCCAGGGCTCGGC CTCCAGGGGGACCTGGACGACTTCCTTCCCCTCATCCATGTGTACCTCCAGTGCAGGACACGGAGCCACT TCACACGCCCAGCAGGAGTGTCTTCCGCTGTCATTCCTGTCTTGAGGAAGACCCTGTGGAGGCAGCTACA GAGCAGGCTTCTTAGCACTGACAGTCCCCCAGCATCTGGGCTGTACCAGGAGATCCTGGCACAGCTGGTC CCGTTTGCACGAGCCAAGGATCTCAGTGTACTCATGGACCGCCTGCCCAGCTTGCTTCACACCCCAAGCA GTCACAAGAGGTGGATCGTGGCCGACTCCATTTCAGCAGCTCTGGAAGGGTCAGCAGAAGAGCTGTGTGC CTGGAGAAGGACCCTTTTGGAGTCCTGTGTGAAGTGGCTGATCGTGTCTTTCAGTGGTGGACAGCAAGAT GATGATAATACTCAGAATCAGGAGAAGGAAATGCTGCTTAGATTAAATGCGTTGTTGCATGCACTTAATG AAGTTGATCCTGGTGACTGGCAGAAATTCGTGAAGAAGGGACTCAAATTTCGGTACCAGGACCACACATT TTTAAAGATGCTCCTCACCGCCGTACAGCTCCTGTACAGCCCAGAAAGCTCCGTGCGCACGAAGCTCATC CAGCTCCCGGTGGTCTACGTGATGCTCATGCAGCACTCGCTGTTTCTGCCGACTCTACTGACGTCTGACG GAGAGGAGAGCCCGGACAGCCAAGTAAAAGAAGCGCTGGTGGACCTGATGCTGACGGTGGTGGAGATGTG CCCCTCTGTCTGTGAGAGCAGCCACTTTGCAGTGCTTCTCGGGGCCTATGGCGCCACTCTCAGCGTCCTA GATCAGAAGATTTTACTTCTGCTTCGAGCGTATGAGCAGAACAAGCTCAGCCTCATCAACTTCAGGGTGC TGCTGTGGGGCCCAGCGGCCGTGGAGCATCACAAGACGTGCCGGAGCCTGGGCAGGTCACTGTGGCAGCA GCCGAGTGTCGGGGACATCCTTCGCCTGCTGGACCGGGACCGGATGATGCAGACCATCCTGCACTTCCCC CAGAACCGGAGGCTGCTGCCCCCCGAGGACACACAGGAGCTGATATTCAAAGACAAAAGCAGGGTGGATC TTGATGGCCTCTATGACCCCTGCTTTCTCCTTCAGCTCTTCAGTGAGCTGACCAGGCCAGAGTTTGTGGT GGATTGTCGAAAATTTTTGGATTCAAATGCTCTGGGCCTAACTGTCACAGCCCTCAGCAGCTATGACCCC CAGATGCGAGCCATAGCCTACCATGTCCTGGCGGCCTACTACTCGCACTTGGAGGGCGCACGGTTCCAAG AGCAGTCCCAGCTACTTTACCTGTTGGATGTAGTCCGGAATGGGATTCGAACTCAGGACATGAGACTTAC TTTTACCTTGGCTCTCTTCATTGCCAAAGCAGCCCTGCAGATTTTGAAACCAGAGGAGCACATGTACCTG AAGGTCAGCAACTTCCTGCTGTCGCATGAGTACTTGAACATGGACAAAGTGCCAGGCTTCTACCAGTTCT TCTACAGCTCCGACTTTGAGCAAAAAACAGAGCAGAAGTGGGTGTTTGGCGTTCTGCGGCAGGGGATCCG TGACAAGCAGTGCTACGAACTGTGTGCCCGGCGTGGCATCTTCCACATCATCCTGTCCTTCTTCCACAGC CCGCTGTGTGACGAGGCAGCACAGAATTGGATTCTGGAAATTCTACAGAATGCTGCCCAGGTTGCCAGAT CTGCGTATGAAATCATACGAGACTATAGCCTTTTAACATGGATTTTACACATCCTTGAAAGCAAGTTTCT GGAGACTCCGCTGCTGTCTAATGTGATCTCCTTGCTACACACACTGTGGGTGACCAACCTGGGGGACAAG GCAGTGGAGTGGGAGAGCCAGCGCCTTTGCCAGCCTAGCTCCCAGGAGCCTGCCAAGCGGCTTGCCCTGC ACCTGGTCAATGAGTTCCTTTATGTTCTCATCGTGCTCATGAAGCACCTGAGGCCCACCTTGGCCCCCGT CCAGCTGACCAACTTCTTCGGGACACTTGACTCCGTGCTGAGGTACCGGGCCACTGTCATACAGGCCTTT AGGGACATGAACAGATTCACCGTAAATGAGACAGTGCTTTCCACCAAGGACGTCCTTGTCCTCTTGCACA AGTGGAGCCTCATTGAAAGAGACCTCAAGCTCCAGGAAGACCTGAGAGCAGCCATTGAGAAGGCCCAAGC CCGGGAGCTCATGAAAATGCTCAAGGATAAGAACAAGCCTGTCATGCCAGCCCGAGCCAAAGGCCCACGG GGCCGAAAGAGGAGGCCTGGGGAGGCAGAGGAGATGGCTGACCCTGAGCTGATGGCATCTACCTTGGAGA CATGCAAGGGCCTCCTGAGGTCCATCTTGACTTACTGGAGACCAGTGATCCCTGGTCCTGACCCCACTCA GGAGCCTGTGGACTCAGCCAGCCCCGAGAGCGATGCGCCAGGCCCCGTATATGCTGCCGCTTCCCTGGCA GTCAGTTGGGTGCTGCGGTCGGTGGCCGAGCACCCGCTCAGCAGGGCAGAGGCTGCAGGACTCATTGGCT GGCTTAAGAGCCATATTTTGCCACACCCTGTGGTCGTGGCTGACCTCCTTAAGGACAGTGCCGTGAGGAG CAGCATATTCAGGCTGTATAGCCGGCTCTGTGGGGCTGAGGGGCTGGCAGGGCCTGTGCAGGAGGTGGCC TGCCTGTTCAATACGGTCATGCTGCAGCTGGTGGCTGCCCAGGGCCGGGCAGGGAGCCCCTTCCACCCGG CCATGGAAGCCCTCTCCCTGTCTTCTCTGAGTGAGAAGGATGAAGCCACACAAGCCTCCGCAGCATTCCT AGTGTCTCTCTACATAAAGGACATCTGGCTGGGGGCTCAGCGGCCGGACACCCTCTTAACCCACGTCCGG ATGGTGTGTGAGGCCGCAGATGATGCCCCGAGCAGTGAAGAGGAGGCCATCGTGGTGCTCTGCAAGGACG CCGCCAGCGCAGCCTCAGATGCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG226482 representing NM_014825
Red=Cloning site Green=Tags(s) MGVPKRKASGGQDGAASSAGAAKRARKEELTGVRFKAQLKDPQGPGPGLEAFVSAAKKLPREDVYDVVEG YIKISVECVEIFQLLSGEKRPESETMLIFQVFEAILLRTASDLSHFHVVGTNIVKKLMNNHMKLICESLY ASGYRLARACLSLMTAMVTQGPEAARDVCSHFDLNKKTLYTLVTKRDSKGVYDVRQAYVQFALSFLIAGD DSTIVQVLEVKEFIPCIFSSGIKEDRISTINILLSTLKTKVVHNKNITKTQKVRFFTGQLLNHIASLYNW NGITDVNPENVKVSAEEAGKTMVRELVHNFLMDLCCSLKHGINFYDASLGTFGRGGNLTLLHFLLGLKTA ADDDLVADLVVNILKVCPDLLNKYFKEVTFSFIPRAKSTWLNNIKLLNKIYEAQPEISRAFQTREFIPLP RLLAMVMVTTVPLVCNKSMFTQALNLDSTSVRHTALSLISVILKRALKTVDHCLNKEVWQESGVYTAVMM EEFVQLFREALSKILPDLNTVVWVWQSLKKQETKQDDKKGQKRSDGPPAACDAHQCDDAETILLKAVLLQ VICLYQKVVPHVVMQYNFDFSKLLKGVISEQGLREEVPPILQHHMLKVALELPASKFLWLKAQEGPDAEI IGGERSVFYLLMKMFVTSSHLQLKSLTKLLIMKILRDTGVFEHTWKELELWLEHLENTMEEDKETVIQFL ERILLTLVANPYSYTDKASDFVQEASMLQATMTKQEADDMSIPISHIDDVLDMVDVLVEGSEGLDEEIGF TLSEDMILLTFPFSAVVPAALEARNKLLLGTGNEAAENVVTYLTAVLTDLLHTQRDPLALCLLLQAYDKL EPPCLVPCCQQLSRFNRYYSLWIPEQAREAWLLQAQGSPSPPALPLASSFTALLQAAYESQALRDEHIQV QLQATMPHLSMQQVLLAAKQVLLYLRSTVENFGQLGRSVGPPLLQLFLDLLRRLVVHCEQLDAQNQQRCE AARAEADLFLDMESVASLELANDQTLEEVLVAILRHPTLEGWFLALEQQALPPHTLSPVLVKLLATHFSA GVLQLLAASAPILQNIGQLGLLARYSEAITQSVLKELQNRRAGPATSPPKTPPQLEALQELHPYMEGAQL REVTLALLSLPETHLVTQQPTKSPGKERHLNALGKTLVQLLTCSPQDQLQSGELLWSSEYVRGLGALLPT LAVDELDTVLLHTLQRDPVLAPAVGADLLDYCLARRTQAALSIAALLLQESCTHLLWFEQWCLQAGPGLG LQGDLDDFLPLIHVYLQCRTRSHFTRPAGVSSAVIPVLRKTLWRQLQSRLLSTDSPPASGLYQEILAQLV PFARAKDLSVLMDRLPSLLHTPSSHKRWIVADSISAALEGSAEELCAWRRTLLESCVKWLIVSFSGGQQD DDNTQNQEKEMLLRLNALLHALNEVDPGDWQKFVKKGLKFRYQDHTFLKMLLTAVQLLYSPESSVRTKLI QLPVVYVMLMQHSLFLPTLLTSDGEESPDSQVKEALVDLMLTVVEMCPSVCESSHFAVLLGAYGATLSVL DQKILLLLRAYEQNKLSLINFRVLLWGPAAVEHHKTCRSLGRSLWQQPSVGDILRLLDRDRMMQTILHFP QNRRLLPPEDTQELIFKDKSRVDLDGLYDPCFLLQLFSELTRPEFVVDCRKFLDSNALGLTVTALSSYDP QMRAIAYHVLAAYYSHLEGARFQEQSQLLYLLDVVRNGIRTQDMRLTFTLALFIAKAALQILKPEEHMYL KVSNFLLSHEYLNMDKVPGFYQFFYSSDFEQKTEQKWVFGVLRQGIRDKQCYELCARRGIFHIILSFFHS PLCDEAAQNWILEILQNAAQVARSAYEIIRDYSLLTWILHILESKFLETPLLSNVISLLHTLWVTNLGDK AVEWESQRLCQPSSQEPAKRLALHLVNEFLYVLIVLMKHLRPTLAPVQLTNFFGTLDSVLRYRATVIQAF RDMNRFTVNETVLSTKDVLVLLHKWSLIERDLKLQEDLRAAIEKAQARELMKMLKDKNKPVMPARAKGPR GRKRRPGEAEEMADPELMASTLETCKGLLRSILTYWRPVIPGPDPTQEPVDSASPESDAPGPVYAAASLA VSWVLRSVAEHPLSRAEAAGLIGWLKSHILPHPVVVADLLKDSAVRSSIFRLYSRLCGAEGLAGPVQEVA CLFNTVMLQLVAAQGRAGSPFHPAMEALSLSSLSEKDEATQASAAFLVSLYIKDIWLGAQRPDTLLTHVR MVCEAADDAPSSEEEAIVVLCKDAASAASDA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014825 |
| ORF Size | 6813 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_014825.3 |
| RefSeq Size | 10808 bp |
| RefSeq ORF | 6816 bp |
| Locus ID | 9875 |
| UniProt ID | O60287 |
| Cytogenetics | 21q22.11 |
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