Desmoglein 4 (DSG4) (NM_001134453) Human Tagged ORF Clone

CAT#: RG226314

DSG4 (tGFP-tagged) - Human desmoglein 4 (DSG4), transcript variant 1

Product Images

Circular map for RG226314

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Desmoglein 4
• Synonyms: CDGF13; CDHF13; HYPT6; LAH
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG226314 representing NM_001134453
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATTGGCTCTTCTTCAGAAACATTTGCCTTTTGATCATTCTAATGGTGGTGATGGAAGTAAACAGTG
  AATTTATTGTTGAGGTGAAGGAATTTGACATTGAAAATGGCACTACAAAATGGCAAACAGTCAGAAGACA
  AAAGCGGGAGTGGATCAAGTTTGCCGCAGCCTGTCGAGAAGGAGAGGACAACTCGAAGAGGAACCCCATT
  GCCAAAATTCGATCAGACTGCGAATCGAACCAGAAGATAACATACCGGATTTCTGGAGTAGGGATTGATC
  GACCACCATATGGGGTATTCACCATTAATCCTCGCACTGGGGAAATTAACATCACTTCAGTGGTAGACAG
  AGAAATAACTCCACTTTTCTTGATCTATTGCCGGGCTCTGAATTCACGGGGTGAAGATTTAGAAAGGCCT
  CTTGAGCTTAGAGTCAAAGTTATGGACATAAATGATAACGCTCCAGTCTTTTCGCAAAGTGTATACACAG
  CCAGCATTGAAGAAAATAGTGATGCCAATACATTGGTAGTAAAGTTATGTGCCACAGATGCAGATGAAGA
  AAATCATCTGAATTCTAAAATTGCCTACAAGATCGTCTCTCAGGAGCCATCAGGTGCACCCATGTTCATT
  CTGAATAGGTACACTGGAGAAGTCTGCACCATGTCCAGTTTCTTGGACAGAGAGCAACACAGTATGTACA
  ACCTGGTTGTGAGAGGCTCAGATCGGGATGGAGCTGCAGATGGACTGTCTTCTGAGTGTGACTGTAGAAT
  CAAGGTTTTAGACGTCAACGATAATTTCCCCACCTTAGAGAAAACTTCATACTCAGCCAGTATTGAAGAG
  AATTGTTTAAGTTCGGAACTGATACGATTACAAGCAATTGATCTTGATGAAGAAGGCACTGATAACTGGT
  TGGCTCAATATTTAATTCTCTCTGGAAATGATGGGAATTGGTTCGATATTCAAACAGATCCACAAACCAA
  TGAAGGCATTTTGAAAGTTGTCAAGATGCTGGATTATGAACAAGCACCTAACATTCAGCTTAGTATCGGA
  GTTAAAAACCAAGCTGATTTTCACTACTCCGTTGCTTCTCAATTCCAAATGCACCCAACCCCTGTGAGAA
  TTCAAGTTGTTGATGTGAGAGAAGGACCTGCATTTCATCCAAGTACTATGGCTTTTAGTGTGCGGGAAGG
  AATAAAAGGAAGTTCCTTATTGAATTATGTGCTTGGCACATATACAGCCATAGATTTGGACACAGGAAAC
  CCTGCAACAGATGTCAGATATATCATAGGGCATGATGCAGGCAGCTGGTTAAAAATTGATTCAAGAACTG
  GTGAGATACAATTTTCTAGAGAATTTGATAAGAAGTCAAAATATATTATCAATGGGATATACACAGCAGA
  GATCCTGGCTATAGATGATGGCTCTGGAAAAACAGCTACAGGAACCATATGTATTGAGGTTCCTGATATC
  AATGATTATTGTCCAAACATTTTTCCTGAAAGAAGAACCATCTGCATTGACTCTCCATCAGTCCTTATCT
  CTGTTAATGAACATTCTTATGGGTCTCCGTTTACTTTCTGTGTTGTTGATGAGCCACCAGGAATAGCTGA
  CATGTGGGATGTCAGATCAACAAATGCTACCTCGGCAATCCTTACGGCTAAGCAGGTTTTATCTCCAGGA
  TTTTATGAAATCCCAATCCTGGTGAAGGACAGCTATAACAGAGCATGTGAATTGGCACAAATGGTGCAGT
  TATATGCCTGTGATTGCGATGACAACCACATGTGCCTGGACTCTGGTGCCGCGGGCATCTACACAGAGGA
  CATAACTGGTGACACGTATGGGCCTGTCACTGAAGACCAAGCTGGAGTTTCAAATGTTGGTCTTGGACCA
  GCAGGGATTGGCATGATGGTTCTGGGCATCCTGCTACTGATTTTGGCTCCACTCTTGCTGCTCCTGTGTT
  GCTGCAAACAGAGACAGCCAGAAGGCCTGGGAACAAGATTTGCTCCTGTGCCTGAGGGCGGAGAAGGAGT
  GATGCAGTCTTGGAGAATTGAAGGGGCCCATCCCGAGGACAGGCTTTTTAGCGCCTACGCCTTGCCGGGT
  GGTGGTGGCACCGCAGACGGCGGCGGCAGCGTTTTAGGCAGATGCGCGCTGCAGGCAACTCCAGCGCTGT
  TAAACCAACATCCTCCCTTTTCAGAAATCTACACCAACACCTATGCAGCCGGGGGCACGGTGGAAGGAGG
  TGTATCGGGAGTGGAGCTCAACACAGGTATGGGGACAGCCGTTGGCCTCATGGCCGCAGGGGCCGCAGGA
  GCCTCAGGGGCCGCAAGGAAGAGGAGCTCTACCATGGGAACCCTGCGGGACTACGCTGACGCAGACATCA
  ACATGGCTTTCTTGGACAGCTACTTCTCGGAGAAAGCGTATGCTTATGCAGATGAAGATGAAGGTCGACC
  AGCCAATGACTGCTTGCTCATTTATGACCACGAGGGAGTCGGGTCTCCCGTAGGCTCTATTGGTTGTTGC
  AGTTGGATTGTGGATGACTTAGATGAAAGCTGCATGGAAACTTTAGATCCAAAATTTAGGACTCTTGCTG
  AGATCTGCTTAAACACAGAAATTGAACCATTTCCTTCACACCAGGCTTGTATACCAATCAGTACTGACCT
  CCCTTTGCTCGGACCTAATTACTTTGTTAATGAATCTTCAGGATTGACTCCCTCAGAAGTTGAATTCCAA
  GAAGAAATGGCAGCATCTGAACCCGTGGTCCATGGGGATATTATTGTGACTGAGACTTACGGTAATGCTG
  ATCCATGTGTGCAACCCACTACAATTATTTTTGATCCTCAGCTTGCACCCAATGTTGTAGTAACCGAAGC
  AGTAATGGCACCTGTCTATGATATTCAAGGGAATATTTGTGTACCTGCTGAGTTAGCAGATTACAACAAT
  GTAATCTATGCTGAGAGAGTACTGGCTAGTCCTGGTGTGCCTGACATGAGCAATAGTAGCACGACTGAGG
  GTTGTATGGGACCTGTGATGAGCGGCAATATTTTAGTAGGGCCAGAAATTCAAGTGATGCAAATGATGAG
  TCCAGACCTTCCCATAGGCCAAACCGTTGGCTCCACATCCCCCATGACATCTCGACACAGAGTAACACGA
  TACAGTAACATACATTACACCCAACAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG226314 representing NM_001134453
  Red=Cloning site Green=Tags(s)
  
  MDWLFFRNICLLIILMVVMEVNSEFIVEVKEFDIENGTTKWQTVRRQKREWIKFAAACREGEDNSKRNPI
  AKIRSDCESNQKITYRISGVGIDRPPYGVFTINPRTGEINITSVVDREITPLFLIYCRALNSRGEDLERP
  LELRVKVMDINDNAPVFSQSVYTASIEENSDANTLVVKLCATDADEENHLNSKIAYKIVSQEPSGAPMFI
  LNRYTGEVCTMSSFLDREQHSMYNLVVRGSDRDGAADGLSSECDCRIKVLDVNDNFPTLEKTSYSASIEE
  NCLSSELIRLQAIDLDEEGTDNWLAQYLILSGNDGNWFDIQTDPQTNEGILKVVKMLDYEQAPNIQLSIG
  VKNQADFHYSVASQFQMHPTPVRIQVVDVREGPAFHPSTMAFSVREGIKGSSLLNYVLGTYTAIDLDTGN
  PATDVRYIIGHDAGSWLKIDSRTGEIQFSREFDKKSKYIINGIYTAEILAIDDGSGKTATGTICIEVPDI
  NDYCPNIFPERRTICIDSPSVLISVNEHSYGSPFTFCVVDEPPGIADMWDVRSTNATSAILTAKQVLSPG
  FYEIPILVKDSYNRACELAQMVQLYACDCDDNHMCLDSGAAGIYTEDITGDTYGPVTEDQAGVSNVGLGP
  AGIGMMVLGILLLILAPLLLLLCCCKQRQPEGLGTRFAPVPEGGEGVMQSWRIEGAHPEDRLFSAYALPG
  GGGTADGGGSVLGRCALQATPALLNQHPPFSEIYTNTYAAGGTVEGGVSGVELNTGMGTAVGLMAAGAAG
  ASGAARKRSSTMGTLRDYADADINMAFLDSYFSEKAYAYADEDEGRPANDCLLIYDHEGVGSPVGSIGCC
  SWIVDDLDESCMETLDPKFRTLAEICLNTEIEPFPSHQACIPISTDLPLLGPNYFVNESSGLTPSEVEFQ
  EEMAASEPVVHGDIIVTETYGNADPCVQPTTIIFDPQLAPNVVVTEAVMAPVYDIQGNICVPAELADYNN
  VIYAERVLASPGVPDMSNSSTTEGCMGPVMSGNILVGPEIQVMQMMSPDLPIGQTVGSTSPMTSRHRVTR
  YSNIHYTQQ
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001134453
• ORF Size: 3177 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001134453.2
• RefSeq Size: 3637 bp
• RefSeq ORF: 3180 bp
• Locus ID: 147409
• UniProt ID: Q86SJ6
• Cytogenetics: 18q12.1
• Protein Families: Transmembrane
• Gene Summary: This gene encodes a member of the desmoglein subgroup of desmosomal cadherins. The encoded preproprotein is proteolytically processed to generate the mature protein. This protein is a transmembrane component of desmosomes and may play a role in cell-cell adhesion in epithelial cells. Mutations in the gene are associated with localized autosomal recessive hypotrichosis and monilethrix, characterized by impaired hair growth. [provided by RefSeq, May 2016]