ADAM29 (NM_001130705) Human Tagged ORF Clone

CAT#: RG226194

ADAM29 (tGFP-tagged) - Human ADAM metallopeptidase domain 29 (ADAM29), transcript variant 4

Product Images

Circular map for RG226194

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ADAM29
• Synonyms: CT73; svph1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG226194 representing NM_001130705
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGATGTTACTCCTGCTGCATTGCCTTGGGGTGTTTCTGTCCTGTTCTGGACACATCCAGGATGAGC
  ACCCCCAATATCACAGCCCTCCGGATGTGGTGATTCCTGTGAGGATAACTGGCACCACCAGAGGCATGAC
  ACCTCCAGGCTGGCTCTCCTATATCCTGCCCTTTGGAGGCCAGAAACACATTATCCACATAAAGGTCAAG
  AAGCTTTTGTTTTCCAAACACCTCCCTGTGTTCACCTACACAGACCAGGGTGCTATCCTTGAGGACCAGC
  CATTTGTCCAGAATAACTGCTACTATCATGGTTATGTGGAAGGGGACCCAGAATCCCTGGTTTCCCTCAG
  TACCTGTTTTGGGGGTTTTCAAGGAATATTACAGATAAATGACTTTGCTTATGAAATCAAGCCCCTAGCA
  TTTTCTACCACGTTTGAACATCTGGTATACAAGATGGACAGTGAGGAGAAACAATTTTCAACCATGAGAT
  CCGGATTTATGCAAAATGAAATAACATGCCGAATGGAATTTGAAGAAATTGATAATTCCACTCAGAAGCA
  AAGTTCTTATGTGGGCTGGTGGATCCATTTTAGGATTGTTGAAATTGTAGTCGTCATTGATAATTATCTG
  TACATTCGTTATGAAAGGAACGACTCAAAGTTGCTGGAGGATCTATATGTTATTGTTAATATAGTGGATT
  CCATTTTGGATGTCATTGGTGTTAAGGTGTTATTATTTGGTTTGGAGATCTGGACCAATAAAAACCTCAT
  TGTAGTAGATGATGTAAGGAAATCTGTGCACCTGTATTGCAAGTGGAAGTCGGAGAACATTACGCCCCGG
  ATGCAACATGACACCTCACATCTTTTCACAACTCTAGGATTAAGAGGGTTAAGTGGCATAGGAGCTTTTA
  GAGGAATGTGTACACCACACCGTAGTTGTGCAATTGTTACTTTCATGAACAAAACTTTGGGCACTTTTTC
  AATTGCAGTGGCTCATCATCTAGGTCATAATTTGGGCATGAACCATGATGAGGATACATGTCGTTGTTCA
  CAACCTAGATGCATAATGCATGAAGGCAACCCACCAATAACTAAATTTAGCAATTGTAGTTATGGTGATT
  TTTGGGAATATACTGTAGAGAGGACAAAGTGTTTGCTTGAAACAGTACACACAAAGGACATCTTTAATGT
  GAAGCGCTGTGGGAATGGTGTTGTTGAAGAAGGAGAAGAGTGTGACTGTGGACCTTTAAAGCATTGTGCA
  AAAGATCCCTGCTGTCTGTCAAATTGCACTCTGACTGATGGTTCTACTTGTGCTTTTGGGCTTTGTTGCA
  AAGACTGCAAGTTCCTACCATCAGGGAAAGTGTGTAGAAAGGAGGTCAATGAATGTGATCTTCCAGAGTG
  GTGCAATGGTACTTCCCATAAGTGCCCAGATGACTTTTATGTGGAAGATGGAATTCCCTGTAAGGAGAGG
  GGCTACTGCTATGAAAAGAGCTGTCATGACCGCAATGAACAGTGTAGGAGGATTTTTGGTGCAGGCGCAA
  ATACTGCAAGTGAGACTTGCTACAAAGAATTGAACACCTTAGGTGACCGTGTTGGTCACTGTGGTATCAA
  AAATGCTACATATATAAAGTGTAATATCTCAGATGTCCAGTGTGGAAGAATTCAGTGTGAGAATGTGACA
  GAAATTCCCAATATGAGTGATCATACTACTGTGCATTGGGCTCGCTTCAATGACATAATGTGCTGGAGTA
  CTGATTACCATTTGGGGATGAAGGGACCTGATATTGGTGAAGTGAAAGATGGAACAGAGTGTGGGATAGA
  TCATATATGCATCCACAGGCACTGTGTCCATATAACCATCTTGAATAGTAATTGCTCACCTGCATTTTGT
  AACAAGAGGGGCATCTGCAACAATAAACATCACTGCCATTGCAATTATCTGTGGGACCCTCCCAACTGCC
  TGATAAAAGGCTATGGAGGTAGTGTTGACAGTGGCCCACCCCCTAAGAGAAAGAAGAAAAAGAAGTTCTG
  TTATCTGTGTATATTGTTGCTTATTGTTTTGTTTATTTTATTATGTTGTCTTTATCGACTTTGTAAAAAA
  AGTAAACCAATAAAAAAGCAGCAAGATGTTCAAACTCCATCTGCAAAAGAAGAGGAAAAAATTCAGCGTC
  GACCTCATGAGTTACCTCCCCAGAGTCAACCTTGGGTGATGCCTTCCCAGAGTCAACCTCCTGTGACGCC
  TTCCCAGAGTCATCCTCAGGTGATGCCTTCCCAGAGTCAACCTCCTGTGACACCCTCCCAGAGTCAACCT
  CGGGTGATGCCTTCTCAGAGTCAACCTCCTGTGATGCCTTCCCAGAGTCATCCTCAGTTGACGCCTTCCC
  AGAGTCAACCTCCTGTGACACCCTCCCAGAGGCAACCTCAGTTGATGCCTTCCCAGAGTCAACCTCCTGT
  GACGCCCTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG226194 representing NM_001130705
  Red=Cloning site Green=Tags(s)
  
  MKMLLLLHCLGVFLSCSGHIQDEHPQYHSPPDVVIPVRITGTTRGMTPPGWLSYILPFGGQKHIIHIKVK
  KLLFSKHLPVFTYTDQGAILEDQPFVQNNCYYHGYVEGDPESLVSLSTCFGGFQGILQINDFAYEIKPLA
  FSTTFEHLVYKMDSEEKQFSTMRSGFMQNEITCRMEFEEIDNSTQKQSSYVGWWIHFRIVEIVVVIDNYL
  YIRYERNDSKLLEDLYVIVNIVDSILDVIGVKVLLFGLEIWTNKNLIVVDDVRKSVHLYCKWKSENITPR
  MQHDTSHLFTTLGLRGLSGIGAFRGMCTPHRSCAIVTFMNKTLGTFSIAVAHHLGHNLGMNHDEDTCRCS
  QPRCIMHEGNPPITKFSNCSYGDFWEYTVERTKCLLETVHTKDIFNVKRCGNGVVEEGEECDCGPLKHCA
  KDPCCLSNCTLTDGSTCAFGLCCKDCKFLPSGKVCRKEVNECDLPEWCNGTSHKCPDDFYVEDGIPCKER
  GYCYEKSCHDRNEQCRRIFGAGANTASETCYKELNTLGDRVGHCGIKNATYIKCNISDVQCGRIQCENVT
  EIPNMSDHTTVHWARFNDIMCWSTDYHLGMKGPDIGEVKDGTECGIDHICIHRHCVHITILNSNCSPAFC
  NKRGICNNKHHCHCNYLWDPPNCLIKGYGGSVDSGPPPKRKKKKKFCYLCILLLIVLFILLCCLYRLCKK
  SKPIKKQQDVQTPSAKEEEKIQRRPHELPPQSQPWVMPSQSQPPVTPSQSHPQVMPSQSQPPVTPSQSQP
  RVMPSQSQPPVMPSQSHPQLTPSQSQPPVTPSQRQPQLMPSQSQPPVTPS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001130705
• ORF Size: 2460 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001130705.1, NP_001124177.1
• RefSeq Size: 3138 bp
• RefSeq ORF: 2463 bp
• Locus ID: 11086
• UniProt ID: Q9UKF5
• Cytogenetics: 4q34.1
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene is highly expressed in testis and may be involved in human spermatogenesis. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Jul 2008]